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J Biol Chem, Vol. 274, Issue 46, 32547-32550, November 12, 1999
COMMUNICATION
Human Lens Coloration and Aging
EVIDENCE FOR CRYSTALLIN MODIFICATION BY THE MAJOR
ULTRAVIOLET FILTER, 3-HYDROXY-KYNURENINE
O- -D-GLUCOSIDE
Brian D.
Hood,
Brett
Garner, and
Roger J. W.
Truscott
From the Australian Cataract Research Foundation, University of
Wollongong, Wollongong, New South Wales 2522, Australia
The human lens becomes increasingly yellow with
age and thereby reduces our perception of blue light. This coloration
is associated with lens proteins (crystallins), but its molecular basis
was unknown. Here we show that the coloration occurs because of the interaction of crystallins with a UV filter compound,
3-hydroxykynurenine glucoside (3-OHKG). Crystallin modification results
from deamination of the 3-OHKG amino acid side chain, yielding an
unsaturated ketone that is susceptible to nucleophilic attack by
cysteine, histidine, and lysine residues. This novel protein
modification contributes to age-related lens coloration and may play a
role in human nuclear cataractogenesis.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
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