| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 46, 32875-32880, November 12, 1999
,,,,,, and
From Mouse vas deferens protein (MVDP) is an aldose
reductase-like protein that is highly expressed in the vas deferens and
adrenal glands and whose physiological functions were unknown. We
hereby describe the enzymatic characteristics of MVDP and its role in murine adrenocortical Y1 cells. The murine aldose reductase (AR) and
MVDP cDNAs were expressed in bacteria to obtain recombinant proteins and to compare their enzymatic activities. Recombinant MVDP
was functional and displayed kinetic properties distinct from those of
murine AR toward various substrates, a preference for NADH, and
insensitivity to AR inhibitors. For MVDP, isocaproaldehyde, a product
of side-chain cleavage of cholesterol generated during steroidogenesis,
is the best natural substrate identified so far. In Y1 cells, we found
that NADH-linked isocaproaldehyde reductase (ICR) activity was much
higher than NADPH-linked ICR activity and was not abolished by AR
inhibitors. We demonstrate that in Y1 cells, forskolin-induced MVDP
expression enhanced NADH-linked ICR activity by 5-6-fold, whereas no
variation in ICR-linked NADPH activity was observed in the same
experiment. In cells stably transfected with MVDP antisense cDNA,
NADH-linked ICR activity was abolished even in the presence of
forskolin, and the isocaproaldehyde toxicity was increased compared
with that of intact Y1 cells, as measured by isocaproaldehyde
LD50. In Y1 cells transfected with MVDP antisense
cDNA, forskolin-induced toxicity was abolished by
aminoglutethimide. These results indicate that in adrenocortical cells,
MVDP is responsible for detoxifying isocaproaldehyde generated by steroidogenesis.
UMR CNRS 6547, Université Blaise Pascal,
Les Cézeaux, 63177 Aubière, Cedex, France and
¶ URA31 CNRS, Université Louis Pasteur,
67000 Strasbourg, France
This article has been cited by other articles:
|
|
 |
|
  C Baumann, B Davies, M Peters, U Kaufmann-Reiche, M Lessl, and F Theuring AKR1B7 (mouse vas deferens protein) is dispensable for mouse development and reproductive success Reproduction, July 1, 2007; 134(1): 97 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tirard, J. Gout, A. M. Lefrancois-Martinez, A. Martinez, M. Begeot, and D. Naville A Novel Inhibitory Protein in Adipose Tissue, the Aldo-Keto Reductase AKR1B7: Its Role in Adipogenesis Endocrinology, May 1, 2007; 148(5): 1996 - 2005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Battista, C. Roberge, M. Otis, and N. Gallo-Payet Seladin-1 expression in rat adrenal gland: effect of adrenocorticotropic hormone treatment J. Endocrinol., January 1, 2007; 192(1): 53 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. O. Ward, D. A. Delker, S. D. Hester, S.-F. Thai, D. C. Wolf, J. W. Allen, and S. Nesnow Transcriptional Profiles in Liver from Mice Treated with Hepatotumorigenic and Nonhepatotumorigenic Triazole Conazole Fungicides: Propiconazole, Triadimefon, and Myclobutanil Toxicol Pathol, December 1, 2006; 34(7): 863 - 878. [Abstract] [PDF]
|
|
|
|
|
|
B. Ragazzon, A.-M. Lefrancois-Martinez, P. Val, I. Sahut-Barnola, C. Tournaire, C. Chambon, J.-L. Gachancard-Bouya, R.-J. Begue, G. Veyssiere, and A. Martinez Adrenocorticotropin-Dependent Changes in SF-1/DAX-1 Ratio Influence Steroidogenic Genes Expression in a Novel Model of Glucocorticoid-Producing Adrenocortical Cell Lines Derived from Targeted Tumorigenesis Endocrinology, April 1, 2006; 147(4): 1805 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Azuma, T. Nishinaka, S. Ushijima, J. Soh, M. Katsuyama, H.-P. Lu, M. Kawata, C. Yabe-Nishimura, and T. Miki Characterization of htAKR, a novel gene product in the aldo-keto reductase family specifically expressed in human testis Mol. Hum. Reprod., July 1, 2004; 10(7): 527 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-M. Lefrancois-Martinez, J. Bertherat, P. Val, C. Tournaire, N. Gallo-Payet, D. Hyndman, G. Veyssiere, X. Bertagna, C. Jean, and A. Martinez Decreased Expression of Cyclic Adenosine Monophosphate-Regulated Aldose Reductase (AKR1B1) Is Associated with Malignancy in Human Sporadic Adrenocortical Tumors J. Clin. Endocrinol. Metab., June 1, 2004; 89(6): 3010 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. Volle, J. J. Repa, A. Mazur, C. L. Cummins, P. Val, J. Henry-Berger, F. Caira, G. Veyssiere, D. J. Mangelsdorf, and J.-M. A. Lobaccaro Regulation of the Aldo-Keto Reductase Gene akr1b7 by the Nuclear Oxysterol Receptor LXR{alpha} (Liver X Receptor-{alpha}) in the Mouse Intestine: Putative Role of LXRs in Lipid Detoxification Processes Mol. Endocrinol., April 1, 2004; 18(4): 888 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Val, C. Aigueperse, B. Ragazzon, G. Veyssiere, A.-M. Lefrancois-Martinez, and A. Martinez Adrenocorticotropin/3',5'-Cyclic AMP-Mediated Transcription of the Scavenger akr1-b7 Gene in Adrenocortical Cells Is Dependent on Three Functionally Distinct Steroidogenic Factor-1-Responsive Elements Endocrinology, February 1, 2004; 145(2): 508 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Martinez, P. Val, I. Sahut-Barnola, C. Aigueperse, G. Veyssiere, and A.-M. Lefrancois-Martinez Steroidogenic Factor-1 Controls the Aldose Reductase akr1b7 Gene Promoter in Transgenic Mice through an Atypical Binding Site Endocrinology, May 1, 2003; 144(5): 2111 - 2120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Val, A. Martinez, I. Sahut-Barnola, C. Jean, G. Veyssiere, and A.-M. Lefrancois-Martinez A 77-Base Pair LINE-Like Sequence Elicits Androgen-Dependent mvdp/akr1-b7 Expression in Mouse Vas Deferens, But Is Dispensable for Adrenal Expression in Rats Endocrinology, September 1, 2002; 143(9): 3435 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kobayashi, T. Kaneko, Y. Iuchi, S. Matsuki, M. Takahashi, I. Sasagawa, T. Nakada, and J. Fujii Localization and Physiological Implication of Aldose Reductase and Sorbitol Dehydrogenase in Reproductive Tracts and Spermatozoa of Male Rats J Androl, September 1, 2002; 23(5): 674 - 683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sarkar, T. Imai, F. Kambe, A. Shibata, S. Ohmori, A. Siddiq, S. Hayasaka, H. Funahashi, and H. Seo The Human Homolog of Diminuto/Dwarf1 Gene (hDiminuto): A Novel ACTH-Responsive Gene Overexpressed in Benign Cortisol-Producing Adrenocortical Adenomas J. Clin. Endocrinol. Metab., November 1, 2001; 86(11): 5130 - 5137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Aigueperse, P. Val, C. Pacot, C. Darne, E. Lalli, P. Sassone-Corsi, G. Veyssiere, C. Jean, and A. Martinez SF-1 (Steroidogenic Factor-1), C/EBP{beta} (CCAAT/Enhancer Binding Protein), and Ubiquitous Transcription Factors NF1 (Nuclear Factor 1) and Sp1 (Selective Promoter Factor 1) Are Required for Regulation of the Mouse Aldose Reductase-Like Gene (AKR1B7) Expression in Adrenocortical Cells Mol. Endocrinol., January 1, 2001; 15(1): 93 - 111. [Abstract] [Full Text]
|
|
|
|
|
|
E. Brockstedt, M. Peters-Kottig, V. Badock, C. Hegele-Hartung, and M. Lessl Luteinizing Hormone Induces Mouse Vas Deferens Protein Expression in the Murine Ovary Endocrinology, July 1, 2000; 141(7): 2574 - 2581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Crosas, E. Cederlund, D. Torres, H. Jornvall, J. Farres, and X. Pares A Vertebrate Aldo-keto Reductase Active with Retinoids and Ethanol J. Biol. Chem., May 25, 2001; 276(22): 19132 - 19140. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
