HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Roques, M. Articles by Vidal, H. Search for Related Content PubMed PubMed Citation Articles by Roques, M. Articles by Vidal, H. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 48, 34005-34010, November 26, 1999

A Phosphatidylinositol 3-Kinase/p70 Ribosomal S6 Protein Kinase Pathway Is Required for the Regulation by Insulin of the p85 Regulatory Subunit of Phosphatidylinositol 3-Kinase Gene Expression in Human Muscle Cells

From INSERM U449, Faculté de Médecine René Laënnec, Université Claude Bernard Lyon-1, F-69372, Lyon, France

Insulin acutely up-regulates p85 phosphatidylinositol 3-kinase (p85PI 3-K) mRNA levels in human skeletal muscle (Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J. P., and Vidal, H. (1996) J. Clin. Invest. 98, 43-49). In the present work, we attempted to elucidate the mechanism of action of insulin in primary cultures of human muscle cells. Insulin (10⁷ M, 6 h of incubation) induced a 2-fold increase in p85PI 3-K mRNA abundances (118 ± 12 versus 233 ± 35 amol/µg total RNA, n = 5, p < 0.01) without changing the expression levels of insulin receptor, IRS-1, glycogen synthase, and Glut 4 mRNAs in differentiated myotubes from healthy subjects. The effect is most probably due to a transcriptional activation of the p85PI 3-K gene because the half-life of the mRNA was not affected by insulin treatment (4.0 ± 0.8 versus 3.1 ± 0.4 h). PD98059 (50 µM) did not modify the insulin response but increased p85PI 3-K mRNA levels in the absence of insulin, suggesting that the mitogen-activated protein kinase pathway exerts a negative effect on p85PI 3-K mRNA expression in the absence of the hormone. On the other hand, the insulin effect was totally abolished by LY294002 (10 µM) and rapamycin (50 nM). In addition, overexpression of a constitutively active protein kinase B increased p85PI 3-K mRNA levels. These results indicate that the phosphatidylinositol 3-kinase/PKB/p70S6 kinase pathway is required for the stimulation by insulin of p85PI 3-K gene expression in human muscle cells.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C. Broholm, O. H. Mortensen, S. Nielsen, T. Akerstrom, A. Zankari, B. Dahl, and B. K. Pedersen Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle J. Physiol., April 15, 2008; 586(8): 2195 - 2201. [Abstract] [Full Text] [PDF]

				M. Moldes, G. Beauregard, M. Faraj, N. Peretti, P.-H. Ducluzeau, M. Laville, R. Rabasa-Lhoret, H. Vidal, and K. Clement Adiponutrin gene is regulated by insulin and glucose in human adipose tissue Eur. J. Endocrinol., September 1, 2006; 155(3): 461 - 468. [Abstract] [Full Text] [PDF]

				J. Rieusset, K. Bouzakri, E. Chevillotte, N. Ricard, D. Jacquet, J.-P. Bastard, M. Laville, and H. Vidal Suppressor of Cytokine Signaling 3 Expression and Insulin Resistance in Skeletal Muscle of Obese and Type 2 Diabetic Patients Diabetes, September 1, 2004; 53(9): 2232 - 2241. [Abstract] [Full Text] [PDF]

				D. Demozay, S. Rocchi, J.-C. Mas, S. Grillo, L. Pirola, C. Chavey, and E. Van Obberghen Fatty Aldehyde Dehydrogenase: POTENTIAL ROLE IN OXIDATIVE STRESS PROTECTION AND REGULATION OF ITS GENE EXPRESSION BY INSULIN J. Biol. Chem., February 20, 2004; 279(8): 6261 - 6270. [Abstract] [Full Text] [PDF]

				Y. Gosmain, E. Lefai, S. Ryser, M. Roques, and H. Vidal Sterol Regulatory Element-Binding Protein-1 Mediates the Effect of Insulin on Hexokinase II Gene Expression in Human Muscle Cells Diabetes, February 1, 2004; 53(2): 321 - 329. [Abstract] [Full Text]

				K. Bouzakri, M. Roques, P. Gual, S. Espinosa, F. Guebre-Egziabher, J.-P. Riou, M. Laville, Y. Le Marchand-Brustel, J.-F. Tanti, and H. Vidal Reduced Activation of Phosphatidylinositol-3 Kinase and Increased Serine 636 Phosphorylation of Insulin Receptor Substrate-1 in Primary Culture of Skeletal Muscle Cells From Patients With Type 2 Diabetes Diabetes, June 1, 2003; 52(6): 1319 - 1325. [Abstract] [Full Text] [PDF]

				S. Patel, P. A. Lochhead, G. Rena, S. Fumagalli, M. Pende, S. C. Kozma, G. Thomas, and C. Sutherland Insulin Regulation of Insulin-like Growth Factor-binding Protein-1 Gene Expression Is Dependent on the Mammalian Target of Rapamycin, but Independent of Ribosomal S6 Kinase Activity J. Biol. Chem., March 15, 2002; 277(12): 9889 - 9895. [Abstract] [Full Text] [PDF]

				K. Eto, T. Yamashita, Y. Tsubamoto, Y. Terauchi, K. Hirose, N. Kubota, S. Yamashita, J. Taka, S. Satoh, H. Sekihara, et al. Phosphatidylinositol 3-Kinase Suppresses Glucose-Stimulated Insulin Secretion by Affecting Post-Cytosolic [Ca2+] Elevation Signals Diabetes, January 1, 2002; 51(1): 87 - 97. [Abstract] [Full Text] [PDF]

				B. Gonzalez, S. Lamas, and E. M. Melian Cooperation between Low Density Lipoproteins and IGF-I in the Promotion of Mitogenesis in Vascular Smooth Muscle Cells Endocrinology, November 1, 2001; 142(11): 4852 - 4860. [Abstract] [Full Text] [PDF]

				J. S. Bogan, A. E. McKee, and H. F. Lodish Insulin-Responsive Compartments Containing GLUT4 in 3T3-L1 and CHO Cells: Regulation by Amino Acid Concentrations Mol. Cell. Biol., July 15, 2001; 21(14): 4785 - 4806. [Abstract] [Full Text] [PDF]

				P.-H. Ducluzeau, N. Perretti, M. Laville, F. Andreelli, N. Vega, J.-P. Riou, and H. Vidal Regulation by Insulin of Gene Expression in Human Skeletal Muscle and Adipose Tissue: Evidence for Specific Defects in Type 2 Diabetes Diabetes, May 1, 2001; 50(5): 1134 - 1142. [Abstract] [Full Text]

				E. Chevillotte, J. Rieusset, M. Roques, M. Desage, and H. Vidal The Regulation of Uncoupling Protein-2 Gene Expression by omega -6 Polyunsaturated Fatty Acids in Human Skeletal Muscle Cells Involves Multiple Pathways, Including the Nuclear Receptor Peroxisome Proliferator-activated Receptor beta J. Biol. Chem., March 30, 2001; 276(14): 10853 - 10860. [Abstract] [Full Text] [PDF]