| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 48, 34417-34424, November 26, 1999
B Kinase Complex
From the Division of Hematology-Oncology, Department of Medicine,
Simmons Cancer Center, University of Texas Southwestern Medical Center,
Dallas, Texas 75235-8594
Two cytokine-inducible kinases, IKK
and
IKK
, are components of a 700-kDa kinase complex that specifically
phosphorylates I
B. Phosphorylation of IB by IKK leads to its
ubiquitination and subsequent degradation, resulting in the nuclear
translocation of NF-B. The oncogenic protein Tax, encoded by human
T-cell leukemia virus type-1 (HTLV-1), stimulates IKK activity to
result in constitutive nuclear levels of NF-B. In an attempt to gain
insights into the mechanism by which Tax mediates constitutive
activation of the NF-B pathway, we analyzed the chromatographic
distribution of IKK proteins using cellular extracts prepared from
three T lymphocytes either lacking or containing Tax. IKK kinase
activity and the distribution of proteins in the IKK complex were
characterized. In extracts prepared from cells containing Tax, the
activity of both IKK and IKK present in the 700-kDa IKK complex
were increased. Surprisingly, cell lines expressing Tax also contained
an additional peak of IKK, but not IKK activity, that migrated at
300 kDa rather than at 700 kDa. We noted that extracts containing Tax had extremely low levels of IB, but not IB, and contained predominantly a truncated form of the MAP3K MEKK1. These results suggest that Tax may target several components of the NF-B pathway leading to constitutive activation of this important regulator of
cellular gene expression.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Dasgupta, K.-J. Jung, S.-J. Jeong, and J. N. Brady Inhibition of Methyltransferases Results in Induction of G2/M Checkpoint and Programmed Cell Death in Human T-Lymphotropic Virus Type 1-Transformed Cells J. Virol., January 1, 2008; 82(1): 49 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Younis, B. Yamamoto, A. Phipps, and P. L. Green Human T-Cell Leukemia Virus Type 1 Expressing Nonoverlapping Tax and Rex Genes Replicates and Immortalizes Primary Human T Lymphocytes but Fails To Replicate and Persist In Vivo J. Virol., December 1, 2005; 79(23): 14473 - 14481. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Lamsoul, J. Lodewick, S. Lebrun, R. Brasseur, A. Burny, R. B. Gaynor, and F. Bex Exclusive Ubiquitination and Sumoylation on Overlapping Lysine Residues Mediate NF-{kappa}B Activation by the Human T-Cell Leukemia Virus Tax Oncoprotein Mol. Cell. Biol., December 1, 2005; 25(23): 10391 - 10406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. Jeong, C. A. Pise-Masison, M. F. Radonovich, H. U. Park, and J. N. Brady A Novel NF-{kappa}B Pathway Involving IKK{beta} and p65/RelA Ser-536 Phosphorylation Results in p53 Inhibition in the Absence of NF-{kappa}B Transcriptional Activity J. Biol. Chem., March 18, 2005; 280(11): 10326 - 10332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wu, M. E. Bottazzi, C. de la Fuente, L. Deng, S. D. Gitlin, A. Maddukuri, S. Dadgar, H. Li, A. Vertes, A. Pumfery, et al. Protein Profile of Tax-associated Complexes J. Biol. Chem., January 2, 2004; 279(1): 495 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lu, C. A. Pise-Masison, T. M. Fletcher, R. L. Schiltz, A. K. Nagaich, M. Radonovich, G. Hager, P. A. Cole, and J. N. Brady Acetylation of Nucleosomal Histones by p300 Facilitates Transcription from Tax-Responsive Human T-Cell Leukemia Virus Type 1 Chromatin Template Mol. Cell. Biol., July 1, 2002; 22(13): 4450 - 4462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cheng, C. Cenciarelli, M. Tao, W. P. Parks, and C. Cheng-Mayer HTLV-1 Tax-associated hTid-1, a Human DnaJ Protein, Is a Repressor of Ikappa B Kinase beta Subunit J. Biol. Chem., May 31, 2002; 277(23): 20605 - 20610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu, M. T. Eby, N. Rathore, S. K. Sinha, A. Kumar, and P. M. Chaudhary The Human Herpes Virus 8-encoded Viral FLICE Inhibitory Protein Physically Associates with and Persistently Activates the Ikappa B Kinase Complex J. Biol. Chem., April 12, 2002; 277(16): 13745 - 13751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Riou, M. Vandromme, and L. Gazzolo Human T-Cell Leukemia Virus Type 1 Tax Protein Inhibits the Expression of the Basic Helix-Loop-Helix Transcription Factor MyoD in Muscle Cells: Maintenance of Proliferation and Repression of Differentiation Cell Growth Differ., December 1, 2001; 12(12): 613 - 622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lamberti, K.-M. Lin, Y. Yamamoto, U. Verma, I. M. Verma, S. Byers, and R. B. Gaynor Regulation of beta -Catenin Function by the Ikappa B Kinases J. Biol. Chem., November 2, 2001; 276(45): 42276 - 42286. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Mogensen and S. R. Paludan Molecular Pathways in Virus-Induced Cytokine Production Microbiol. Mol. Biol. Rev., March 1, 2001; 65(1): 131 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Khoshnan, C. Tindell, I. Laux, D. Bae, B. Bennett, and A. E. Nel The NF-{kappa}B Cascade Is Important in Bcl-xL Expression and for the Anti-Apoptotic Effects of the CD28 Receptor in Primary Human CD4+ Lymphocytes J. Immunol., August 15, 2000; 165(4): 1743 - 1754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. T. Kwak, J. Guo, J. Shen, and R. B. Gaynor Analysis of Domains in the IKKalpha and IKKbeta Proteins That Regulate Their Kinase Activity J. Biol. Chem., May 5, 2000; 275(19): 14752 - 14759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-H. Li, X. Fang, and R. B. Gaynor Role of IKKgamma /NEMO in Assembly of the Ikappa B Kinase Complex J. Biol. Chem., February 2, 2001; 276(6): 4494 - 4500. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
