| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 49, 34961-34966, December 3, 1999
From the The influence of mRNA localization on
metallothionein-1 protein distribution was studied by
immunocytochemistry. We used Chinese hamster ovary cells that had been
transfected with either a native metallothionein-1 gene construct or
metallothionein-1 5'-untranslated region and coding sequences linked to
the 3'-untranslated region from glutathione peroxidase. The change in
the 3'-untranslated region caused the delocalization of the mRNA
with a loss of the perinuclear localization and association with the
cytoskeleton. Clones were selected which expressed similar levels of
metallothionein-1 protein, as assessed by radioimmunoassay. The results
showed that loss of metallothionein-1 mRNA localization was
associated with a loss of metallothionein-1 protein localization, most
notably with a lack of metallothionein-1 protein in the nucleus of
synchronized cells which were beginning to synthesize DNA. This
indicates that the association of metallothionein-1 mRNA with the
cytoskeleton around the nucleus is essential for efficient shuttling of
the protein into the nucleus during the G1 to S phase
transition. This is the first demonstration of a physiological role for
perinuclear mRNA localization and we propose that such localization
may be important for a wide range of nuclear proteins, including those that shuttle between nucleus and cytoplasm in a cell cycle dependent manner.
Nuclear Import of Metallothionein Requires Its mRNA to Be
Associated with the Perinuclear Cytoskeleton
,,,
Rowett Research Institute, Greenburn Road,
Bucksburn, Aberdeen AB21 9SB, United Kingdom and the
¶ Department of Medicine and Therapeutics and Biomedical Sciences,
University of Aberdeen, Polwarth Building, Foresterhill,
Aberdeen AB25 2ZD, United Kingdom
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Mickleburgh, H. Chabanon, D. Nury, K. Fan, B. Burtle, Z. Chrzanowska-Lightowlers, and J. Hesketh Elongation factor 1{alpha} binds to the region of the metallothionein-1 mRNA implicated in perinuclear localization--importance of an internal stem-loop RNA, July 1, 2006; 12(7): 1397 - 1407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Bullock, M. Stauber, A. Prell, J. R. Hughes, D. Ish-Horowicz, and U. Schmidt-Ott Differential cytoplasmic mRNA localisation adjusts pair-rule transcription factor activity to cytoarchitecture in dipteran evolution Development, September 1, 2004; 131(17): 4251 - 4261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. G. Jeong, C.-K. Youn, H.-J. Cho, S.-H. Kim, M.-H. Kim, H.-B. Kim, I.-Y. Chang, Y.-S. Lee, M.-H. Chung, and H. J. You Metallothionein-III Prevents {gamma}-Ray-induced 8-Oxoguanine Accumulation in Normal and hOGG1-depleted Cells J. Biol. Chem., August 13, 2004; 279(33): 34138 - 34149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Abu-Hayyeh, M. Sian, K. G. Jones, A. Manuel, and J. T. Powell Cadmium Accumulation in Aortas of Smokers Arterioscler. Thromb. Vasc. Biol., May 1, 2001; 21(5): 863 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dalgleish, J.-L. Veyrune, J.-M. Blanchard, and J. Hesketh mRNA Localization by a 145-Nucleotide Region of the c-fos 3'- Untranslated Region. LINKS TO TRANSLATION BUT NOT STABILITY J. Biol. Chem., April 20, 2001; 276(17): 13593 - 13599. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
