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J Biol Chem, Vol. 274, Issue 5, 3017-3025, January 29, 1999

Isolation, Cloning, and Characterization of a New Mammalian Coronin Family Member, Coronin_se, Which Is Regulated within the Protein Kinase C Signaling Pathway

From the Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912-3175

In order to understand the regulatory role of protein kinase C (PKC) in secretory epithelia, it is necessary to identify and characterize specific downstream targets. We previously identified one such protein in studies of gastric parietal cells. This protein was referred to as pp66 because it migrated with an apparent molecular mass of 66 kDa on SDS-polyacrylamide gels. The phosphorylation of pp66 is increased by the cholinergic agonist, carbachol, and by the PKC activator, phorbol-12-myristate-13-acetate, in a calcium-independent manner. In this study, we have purified pp66 to homogeneity and cloned the complete open reading frame. GenBank^TM searches revealed a 45% homology with the Dictyostelium actin-binding protein, coronin, and ~67% homology with the previously cloned human and bovine coronin-like homologue, p57. pp66 appears to be most highly expressed in the gastrointestinal mucosa and in kidney and lung. Confocal microscopic studies of an enhanced green fluorescent protein fusion construct of pp66 in cultured parietal cells and in Madin-Darby canine kidney cells indicate that pp66 preferentially localizes in F-actin-rich regions. On the basis of our findings, we propose that pp66 may play an important, PKC-dependent role in regulating membrane/cytoskeletal rearrangements in epithelial cells. We have tentatively named this protein coronin_se, because it appears to be highly expressed in secretory epithelia.

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