| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 5, 3116-3124, January 29, 1999
From the Ligation of either CD80 (B7-1) or CD86 (B7-2),
two principal ligands for CD28, is thought to skew the immune response
toward Th1 or Th2 differentiation. We have examined early signal
transduction pathways recruited following T cell stimulation with
either CD80 or CD86. Purified human peripheral T cells or Jurkat T
cells were stimulated with Chinese hamster ovary (CHO) cells expressing
either human CD80 (CHO-CD80) or human CD86 (CHO-CD86) or with anti-CD28 monoclonal antibody (mAb). In the presence of phorbol 12-myristate 13-acetate, both CHO-CD80 and CHO-CD86, like anti-CD28 mAb, were capable of stimulating cytokine production from both human peripheral T
cells and Jurkat T cells. Both CHO-CD80 and CHO-CD86, in the presence
of anti-CD3 mAb, costimulated NFAT-dependent
transcriptional activation. Several intracellular signaling proteins,
such as CBL and VAV, were phosphorylated on tyrosine in response to
CD80, CD86, and anti-CD28 mAb. Surprisingly, although stimulation of Jurkat T cells with either CHO-CD80 or anti-CD28 mAb resulted in robust
tyrosine phosphorylation of CD28 itself, ligation with CHO-CD86 was
unable to induce detectable CD28 tyrosyl phosphorylation over a range
of stimulation conditions. In addition, the association of
phosphoinositide 3-kinase with CD28 and enhanced tyrosine
phosphorylation of phospholipase C
CD80 and CD86 Are Not Equivalent in Their Ability to Induce the
Tyrosine Phosphorylation of CD28
,§, and¶
Department of Pediatric Oncology,
were seen after anti-CD28 mAb and
CHO-CD80 stimulation but to a much lesser extent after CHO-CD86
stimulation. Thus, ligation of CD28 with either CD80 or CD86 leads to
shared early signal transduction events such as the tyrosine
phosphorylation of CBL and VAV, to NFAT-mediated transcriptional
activation, and to the costimulation of interleukin-2 and
granulocyte-macrophage colony-stimulating factor production. However,
CD80 and CD86 also induce distinct signal transduction pathways
including the tyrosine phosphorylation of CD28 and phospholipase C1
and the SH2-dependent association of phosphoinositide
3-kinase with CD28. These quantitative, if not qualitative, differences
between signaling initiated by these two ligands for CD28 may
contribute to functional differences (e.g. Th1 or Th2
differentiation) in T cell responses.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Pejawar-Gaddy and M. A. Alexander-Miller Ligation of CD80 Is Critical for High-Level CD25 Expression on CD8+ T Lymphocytes J. Immunol., October 1, 2006; 177(7): 4495 - 4502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hussain and T. L. Delovitch Dysregulated B7-1 and B7-2 Expression on Nonobese Diabetic Mouse B Cells Is Associated with Increased T Cell Costimulation and the Development of Insulitis J. Immunol., January 15, 2005; 174(2): 680 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Yadav, V. Judkowski, M. Flodstrom-Tullberg, L. Sterling, W. L. Redmond, L. Sherman, and N. Sarvetnick B7-2 (CD86) Controls the Priming of Autoreactive CD4 T Cell Response against Pancreatic Islets J. Immunol., September 15, 2004; 173(6): 3631 - 3639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. E. Lewis, M. Merched-Sauvage, J. J. Goronzy, C. M. Weyand, and A. N. Vallejo Tumor Necrosis Factor-{alpha} and CD80 Modulate CD28 Expression through a Similar Mechanism of T-cell Receptor-independent Inhibition of Transcription J. Biol. Chem., July 9, 2004; 279(28): 29130 - 29138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nierkens, P. van Helden, M. Bol, R. Bleumink, P. van Kooten, S. Ramdien-Murli, L. Boon, and R. Pieters Selective Requirement for CD40-CD154 in Drug-Induced Type 1 Versus Type 2 Responses to Trinitrophenyl-Ovalbumin J. Immunol., April 15, 2002; 168(8): 3747 - 3754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. LaBelle, C. A. Hanke, B. R. Blazar, and R. L. Truitt Negative effect of CTLA-4 on induction of T-cell immunity in vivo to B7-1+, but not B7-2+, murine myelogenous leukemia Blood, March 15, 2002; 99(6): 2146 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Slavik, D.-G. Lim, S. J. Burakoff, and D. A. Hafler Uncoupling p70s6 Kinase Activation and Proliferation: Rapamycin-Resistant Proliferation of Human CD8+ T Lymphocytes J. Immunol., March 1, 2001; 166(5): 3201 - 3209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Ellis, C. Ashman, M. N. Burden, K. E. Kilpatrick, M. A. Morse, and P. A. Hamblin GRID: A Novel Grb-2-Related Adapter Protein That Interacts with the Activated T Cell Costimulatory Receptor CD28 J. Immunol., June 1, 2000; 164(11): 5805 - 5814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Posey, M. P. Martelli, T. Azuma, D. J. Kwiatkowski, and B. E. Bierer Failure of gelsolin overexpression to regulate lymphocyte apoptosis Blood, June 1, 2000; 95(11): 3483 - 3488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Vallejo, L. O. Mugge, P. A. Klimiuk, C. M. Weyand, and J. J. Goronzy Central Role of Thrombospondin-1 in the Activation and Clonal Expansion of Inflammatory T Cells J. Immunol., March 15, 2000; 164(6): 2947 - 2954. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
