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J Biol Chem, Vol. 274, Issue 5, 3151-3158, January 29, 1999
From the Division of Endocrinology, Cedars-Sinai Research
Institute, UCLA School of Medicine,
Los Angeles, California 90048
Pituitary tumor-transforming gene (PTTG) is a
recently characterized proto-oncogene that is expressed specifically in
adult testis. In this study, we have used in situ
hybridization and developmental Northern blot assays to demonstrate
that PTTG mRNA is expressed stage-specifically in spermatocytes and
spermatids during rat spermatogenic cycle. We have used the yeast
two-hybrid system to identify proteins that interact with PTTG in
testicular cells. Two positive clones were characterized. One of the
clones is the ribosomal protein S10, the other encodes a novel human DnaJ homologue designated HSJ2. Northern blot analysis showed that
testis contains higher levels of HSJ2 mRNA than other tissues examined, and the expression pattern of HSJ2 mRNA in postnatal rat
testis is similar to PTTG. S10 mRNA levels do not vary remarkably among different tissues and remains unchanged during testicular germ
cell differentiation. In vitro binding assays demonstrated that both S10 and HSJ2 bind to PTTG specifically and that PTTG can be
co-immunoprecipitated with S10 and HSJ2 from transfected cells.
Moreover, the binding sites for both proteins were located within the
C-terminal 75 amino acids of the PTTG protein. These results suggest
that PTTG may play a role in spermatogenesis.
Pituitary Tumor-transforming Gene Protein Associates with
Ribosomal Protein S10 and a Novel Human Homologue of DnaJ in
Testicular Cells
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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