| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 50, 35301-35304, December 10, 1999
From the Division of Cellular Biochemistry, The Netherlands
Cancer Institute and Centre for Biomedical Genetics, Plesmanlaan
121, 1066CX Amsterdam, The Netherlands
Internalization of activated receptors from the
plasma membrane has been implicated in the activation of
mitogen-activated protein (MAP) kinase. However, the mechanism whereby
membrane trafficking may regulate mitogenic signaling remains unclear. Here we report that dominant-negative dynamin (K44A), an inhibitor of
endocytic vesicle formation, abrogates MAP kinase activation in
response to epidermal growth factor, lysophosphatidic acid, and protein
kinase C-activating phorbol ester. In contrast, dynamin-K44A does not
affect the activation of Ras, Raf, and MAP kinase kinase (MEK) by
either agonist. Through immunofluorescence and subcellular fractionation studies, we find that activated MEK is present both at
the plasma membrane and in intracellular vesicles but not in the
cytosol. Our findings suggest that dynamin-regulated endocytosis of
activated MEK, rather than activated receptors, is a critical event in
the MAP kinase activation cascade.
To whom correspondence should be addressed. Tel.: +31-20-512-1971;
Fax: +31-20-512-1989; E-mail: wmoolen@nki.nl.
This article has been cited by other articles:
|
|
 |
|
  S. A. Parsons and P. A. Greer The Fps/Fes kinase regulates the inflammatory response to endotoxin through down-regulation of TLR4, NF-{kappa}B activation, and TNF-{alpha} secretion in macrophages J. Leukoc. Biol., December 1, 2006; 80(6): 1522 - 1528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. N. Nguyen, M. S. Holdren, A. P. Nguyen, M. H. Furuya, M. Bianchini, E. Levy, J. Mordoh, A. Liu, G. D. Guncay, J. S. Campbell, et al. Sorting Nexin 1 Down-Regulation Promotes Colon Tumorigenesis Clin. Cancer Res., December 1, 2006; 12(23): 6952 - 6959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Hopper The Adaptor Protein soc-1/Gab1 Modifies Growth Factor Receptor Output in Caenorhabditis elegans Genetics, May 1, 2006; 173(1): 163 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Jimenez-Sainz, C. Murga, A. Kavelaars, M. Jurado-Pueyo, B. F. Krakstad, C. J. Heijnen, F. Mayor Jr., and A. M. Aragay G Protein-coupled Receptor Kinase 2 Negatively Regulates Chemokine Signaling at a Level Downstream from G Protein Subunits Mol. Biol. Cell, January 1, 2006; 17(1): 25 - 31. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Soulet, D. Yarar, M. Leonard, and S. L. Schmid SNX9 Regulates Dynamin Assembly and Is Required for Efficient Clathrin-mediated Endocytosis Mol. Biol. Cell, April 1, 2005; 16(4): 2058 - 2067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gendron, A. Perron, M. D. Payet, N. Gallo-Payet, P. Sarret, and A. Beaudet Low-Affinity Neurotensin Receptor (NTS2) Signaling: Internalization-Dependent Activation of Extracellular Signal-Regulated Kinases 1/2 Mol. Pharmacol., December 1, 2004; 66(6): 1421 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Choi, W.-P. Hong, M. J. Kim, J. H. Kim, S. H. Ryu, and P.-G. Suh Sorting nexin 16 regulates EGF receptor trafficking by phosphatidylinositol-3-phosphate interaction with the Phox domain J. Cell Sci., August 15, 2004; 117(18): 4209 - 4218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Choi, J. B. Park, S. S. Bae, S. Yun, H. S. Kim, W.-P. Hong, I.-S. Kim, J. H. Kim, M. Y. Han, S. H. Ryu, et al. Phospholipase C-{gamma}1 is a guanine nucleotide exchange factor for dynamin-1 and enhances dynamin-1-dependent epidermal growth factor receptor endocytosis J. Cell Sci., August 1, 2004; 117(17): 3785 - 3795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Mishra, S. S. Jatiani, A. Kumar, V. R. Simhadri, R. V. Hosur, and R. Mittal Dynamin Interacts with Members of the Sumoylation Machinery J. Biol. Chem., July 23, 2004; 279(30): 31445 - 31454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Beom, D. Cheong, G. Torres, M. G. Caron, and K.-M. Kim Comparative Studies of Molecular Mechanisms of Dopamine D2 and D3 Receptors for the Activation of Extracellular Signal-regulated Kinase J. Biol. Chem., July 2, 2004; 279(27): 28304 - 28314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huang, Y. Sun, and X.-Y. Huang Distinct Roles for Src Tyrosine Kinase in {beta}2-Adrenergic Receptor Signaling to MAPK and in Receptor Internalization J. Biol. Chem., May 14, 2004; 279(20): 21637 - 21642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Kahlig, J. A. Javitch, and A. Galli Amphetamine Regulation of Dopamine Transport: COMBINED MEASUREMENTS OF TRANSPORTER CURRENTS AND TRANSPORTER IMAGING SUPPORT THE ENDOCYTOSIS OF AN ACTIVE CARRIER J. Biol. Chem., March 5, 2004; 279(10): 8966 - 8975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Kim, M. Y. Kim, E. J. Lee, Y. S. Ahn, and J.-H. Baik Distinct Regulation of Internalization and Mitogen-Activated Protein Kinase Activation by Two Isoforms of the Dopamine D2 Receptor Mol. Endocrinol., March 1, 2004; 18(3): 640 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Schlunck, H. Damke, W. B. Kiosses, N. Rusk, M. H. Symons, C. M. Waterman-Storer, S. L. Schmid, and M. A. Schwartz Modulation of Rac Localization and Function by Dynamin Mol. Biol. Cell, January 1, 2004; 15(1): 256 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Jimenez-Sainz, B. Fast, F. Mayor Jr., and A. M. Aragay Signaling Pathways for Monocyte Chemoattractant Protein 1-Mediated Extracellular Signal-Regulated Kinase Activation Mol. Pharmacol., September 1, 2003; 64(3): 773 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Foster and L. Xu Phospholipase D in Cell Proliferation and Cancer Mol. Cancer Res., September 1, 2003; 1(11): 789 - 800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. N Kholodenko Four-dimensional organization of protein kinase signaling cascades: the roles of diffusion, endocytosis and molecular motors J. Exp. Biol., June 15, 2003; 206(12): 2073 - 2082. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Murph, L. A. Scaccia, L. A. Volpicelli, and H. Radhakrishna Agonist-induced endocytosis of lysophosphatidic acid-coupled LPA1/EDG-2 receptors via a dynamin2- and Rab5-dependent pathway J. Cell Sci., May 15, 2003; 116(10): 1969 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. N. van Leeuwen, C. Olivo, S. Grivell, B. N. G. Giepmans, J. G. Collard, and W. H. Moolenaar Rac Activation by Lysophosphatidic Acid LPA1 Receptors through the Guanine Nucleotide Exchange Factor Tiam1 J. Biol. Chem., January 3, 2003; 278(1): 400 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Chiarugi, P. Cirri, M. L. Taddei, E. Giannoni, T. Fiaschi, F. Buricchi, G. Camici, G. Raugei, and G. Ramponi Insight into the Role of Low Molecular Weight Phosphotyrosine Phosphatase (LMW-PTP) on Platelet-derived Growth Factor Receptor (PDGF-r) Signaling. LMW-PTP CONTROLS PDGF-r KINASE ACTIVITY THROUGH TYR-857 DEPHOSPHORYLATION J. Biol. Chem., September 27, 2002; 277(40): 37331 - 37338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Roy, B. Wyse, and J. F. Hancock H-Ras Signaling and K-Ras Signaling Are Differentially Dependent on Endocytosis Mol. Cell. Biol., July 15, 2002; 22(14): 5128 - 5140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Szaszak, Z. Gaborik, G. Turu, P. S. McPherson, A. J. L. Clark, K. J. Catt, and L. Hunyady Role of the Proline-rich Domain of Dynamin-2 and Its Interactions with Src Homology 3 Domains during Endocytosis of the AT1 Angiotensin Receptor J. Biol. Chem., June 7, 2002; 277(24): 21650 - 21656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Seto, H. J. Bellen, and T. E. Lloyd When cell biology meets development: endocytic regulation of signaling pathways Genes & Dev., June 1, 2002; 16(11): 1314 - 1336. [Full Text] [PDF]
|
|
|
|
|
|
S. J. DiCamillo, I. Carreras, M. V. Panchenko, P. J. Stone, M. A. Nugent, J. A. Foster, and M. P. Panchenko Elastase-released Epidermal Growth Factor Recruits Epidermal Growth Factor Receptor and Extracellular Signal-regulated Kinases to Down-regulate Tropoelastin mRNA in Lung Fibroblasts J. Biol. Chem., May 17, 2002; 277(21): 18938 - 18946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Chiarugi, P. Cirri, M. L. Taddei, D. Talini, L. Doria, T. Fiaschi, F. Buricchi, E. Giannoni, G. Camici, G. Raugei, et al. New perspectives in PDGF receptor downregulation: the main role of phosphotyrosine phosphatases J. Cell Sci., May 15, 2002; 115(10): 2219 - 2232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Hislop, H. M. Everest, A. Flynn, T. Harding, J. B. Uney, B. E. Troskie, R. P. Millar, and C. A. McArdle Differential Internalization of Mammalian and Non-mammalian Gonadotropin-releasing Hormone Receptors. UNCOUPLING OF DYNAMIN-DEPENDENT INTERNALIZATION FROM MITOGEN-ACTIVATED PROTEIN KINASE SIGNALING J. Biol. Chem., October 19, 2001; 276(43): 39685 - 39694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Smith, R. Yu, and P. M. Hinkle Activation of MAPK by TRH Requires Clathrin-Dependent Endocytosis and PKC but Not Receptor Interaction with {beta}-Arrestin or Receptor Endocytosis Mol. Endocrinol., September 1, 2001; 15(9): 1539 - 1548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Garamszegi, J. J. E. Dore Jr., S. G. Penheiter, M. Edens, D. Yao, and E. B. Leof Transforming Growth Factor beta Receptor Signaling and Endocytosis Are Linked through a COOH Terminal Activation Motif in the Type I Receptor Mol. Biol. Cell, September 1, 2001; 12(9): 2881 - 2893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wunderlich, I. Fialka, D. Teis, A. Alpi, A. Pfeifer, R. G. Parton, F. Lottspeich, and L. A. Huber A Novel 14-Kilodalton Protein Interacts with the Mitogen-activated Protein Kinase Scaffold MP1 on a Late Endosomal/Lysosomal Compartment J. Cell Biol., February 20, 2001; 152(4): 765 - 776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shen, L. Xu, and D. A. Foster Role for Phospholipase D in Receptor-Mediated Endocytosis Mol. Cell. Biol., January 15, 2001; 21(2): 595 - 602. [Abstract] [Full Text]
|
|
|
|
|
|
A. A. de Melker, G. van der Horst, J. Calafat, H. Jansen, and J. Borst c-Cbl ubiquitinates the EGF receptor at the plasma membrane and remains receptor associated throughout the endocytic route J. Cell Sci., January 6, 2001; 114(11): 2167 - 2178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Urbé, I. G. Mills, H. Stenmark, N. Kitamura, and M. J. Clague Endosomal Localization and Receptor Dynamics Determine Tyrosine Phosphorylation of Hepatocyte Growth Factor-Regulated Tyrosine Kinase Substrate Mol. Cell. Biol., October 15, 2000; 20(20): 7685 - 7692. [Abstract] [Full Text]
|
|
|
|
|
|
K. N. Fish, S. L. Schmid, and H. Damke Evidence that Dynamin-2 Functions as a Signal-transducing GTPase J. Cell Biol., July 11, 2000; 150(1): 145 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.A. DeFea, J. Zalevsky, M.S. Thoma, O. Dery, R.D. Mullins, and N.W. Bunnett {beta}-Arrestin-dependent Endocytosis of Proteinase-activated Receptor 2 Is Required for Intracellular Targeting of Activated ERK1/2 J. Cell Biol., March 20, 2000; 148(6): 1267 - 1282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-K. Tong, N. K. Hussain, A. G. Adams, J. P. O'Bryan, and P. S. McPherson Intersectin Can Regulate the Ras/MAP Kinase Pathway Independent of Its Role in Endocytosis J. Biol. Chem., September 15, 2000; 275(38): 29894 - 29899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Benard, Z. Naor, and R. Seger Role of Dynamin, Src, and Ras in the Protein Kinase C-mediated Activation of ERK by Gonadotropin-releasing Hormone J. Biol. Chem., February 9, 2001; 276(7): 4554 - 4563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Pierce, A. Tohgo, S. Ahn, M. E. Field, L. M. Luttrell, and R. J. Lefkowitz Epidermal Growth Factor (EGF) Receptor-dependent ERK Activation by G Protein-coupled Receptors. A CO-CULTURE SYSTEM FOR IDENTIFYING INTERMEDIATES UPSTREAM AND DOWNSTREAM OF HEPARIN-BINDING EGF SHEDDING J. Biol. Chem., June 15, 2001; 276(25): 23155 - 23160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Schmidlin, O. Dery, K. O. DeFea, L. Slice, S. Patierno, C. Sternini, E. F. Grady, and N. W. Bunnett Dynamin and Rab5a-dependent Trafficking and Signaling of the Neurokinin 1 Receptor J. Biol. Chem., June 29, 2001; 276(27): 25427 - 25437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Rizzo, C. A. Kraft, S. C. Watkins, E. S. Levitan, and G. Romero Agonist-dependent Traffic of Raft-associated Ras and Raf-1 Is Required for Activation of the Mitogen-activated Protein Kinase Cascade J. Biol. Chem., September 7, 2001; 276(37): 34928 - 34933. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Saunders, J. V. Ferrer, L. Shi, J. Chen, G. Merrill, M. E. Lamb, L. M. F. Leeb-Lundberg, L. Carvelli, J. A. Javitch, and A. Galli Amphetamine-induced loss of human dopamine transporter activity: An internalization-dependent and cocaine-sensitive mechanism PNAS, June 6, 2000; 97(12): 6850 - 6855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pawlak and D. M. Helfman Post-transcriptional Down-regulation of ROCKI/Rho-kinase through an MEK-dependent Pathway Leads to Cytoskeleton Disruption in Ras-transformed Fibroblasts Mol. Biol. Cell, January 1, 2002; 13(1): 336 - 347. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
