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J Biol Chem, Vol. 274, Issue 50, 35621-35629, December 10, 1999

DFak56 Is a Novel Drosophila melanogaster Focal Adhesion Kinase*

Ruth H. PalmerDagger §, Liselotte I. Fessler||, Philip T. EdeenDagger , Sanford J. MadiganDagger , Michael McKeownDagger **, and Tony HunterDagger Dagger Dagger

From the Dagger  Salk Institute, Molecular Biology and Virology Laboratory, La Jolla, California 92037-1099 and the  Department of Biology and the Molecular, Cell, and Developmental Biology Institute, University of California, Los Angeles, California 90095-1606

The mammalian focal adhesion kinase (FAK) family of nonreceptor protein-tyrosine kinases have been implicated in controlling a multitude of cellular responses to the engagement of cell surface integrins and G protein-coupled receptors. We describe here a Drosophila melanogaster FAK homologue, DFak56, which maps to band 56D on the right arm of the second chromosome. Full-length DFak56 cDNA encodes a phosphoprotein of 140 kDa, which shares strong sequence similarity not only with mammalian p125FAK but also with the more recently described mammalian Pyk2 (also known as CAKbeta , RAFTK, FAK2, and CADTK) FAK family member. DFak56 has intrinsic tyrosine kinase activity and is phosphorylated on tyrosine in vivo. As is the case for FAK, tyrosine phosphorylation of DFak56 is increased upon plating Drosophila embryo cells on extracellular matrix proteins. In situ hybridization and immunofluorescence staining analysis showed that DFak56 is ubiquitously expressed with particularly high levels within the developing central nervous system. We utilized the UAS-GAL4 expression system to express DFak56 and analyze its function in vivo. Overexpression of DFak56 in the wing imaginal disc results in wing blistering in adults, a phenotype also observed with both position-specific integrin loss of function and position-specific integrin overexpression. Our results imply a role for DFak56 in adhesion-dependent signaling pathways in vivo during D. melanogaster development.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF112116.

§ Supported by a Human Frontiers Science Program Fellowship.

|| Supported by National Institutes of Health Grant GM57689.

** Supported by National Institutes of Health Grant MH57460.

Dagger Dagger Supported by National Institutes of Health Grant CA39780. Frank and Else Schilling American Cancer Society Research Professor. To whom correspondence should be addressed: Salk Inst., Molecular Biology and Virology Laboratory, 10010 North Torrey Pines Rd., La Jolla, CA 92037-1099. Tel.: 858-453-4100, Ext. 1385; Fax: 858-457-4765; E-mail: hunter@salk.edu.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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