| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 50, 35719-35724, December 10, 1999
From the Eukaryotic Genetics Group, Biotechnology Research
Institute, National Research Council, the Department of Anatomy and
Cell Biology, Montreal, H4P 2R2 Quebec, Canada and the
¶ Biology Department, McGill University,
Montreal, H3A 2B2 Quebec, Canada
Grb10 belongs to a small family of adapter
proteins that are known to interact with a number of receptor tyrosine
kinases and signaling molecules. We have recently demonstrated that the Grb10 SH2 domain interacts with both the Raf-1 and MEK1 kinases. Overexpression of Grb10 genes with mutations in their SH2 domains promotes apoptosis in cultured cells, a phenotype that is reversed by
concomitant overexpression of the wild type gene. Using
immunofluorescence microscopy and subcellular fractionation we now show
that most of the Grb10 molecules are peripherally associated with
mitochondria. Following insulin-like growth factor I or serum
treatment, small pools of Grb10 can also be found at the plasma
membrane and in actin-rich membrane ruffles, whereas overexpression of
Grb10 leads to its mislocalization to the cytosol. Two-hybrid analysis
shows that the Grb10-binding site on Raf-1 co-localizes with its
Ras-binding domain. Finally, we show that the endogenous Grb10 and
Raf-1 proteins can be co-immunoprecipitated from a partially purified
mitochondrial extract, an interaction that is enhanced following the
activation of Raf-1 by ultraviolet radiation. Thus, we infer that Grb10
may regulate signaling between plasma membrane receptors and the
apoptosis-inducing machinery on the mitochondrial outer membrane by
modulating the anti-apoptotic activity of mitochondrial Raf-1.
Localization of Endogenous Grb10 to the Mitochondria and Its
Interaction with the Mitochondrial-associated Raf-1 Pool*
,
*
This is National Research Council Publication 41480.The costs of publication of this
article were defrayed in part by the payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Eukaryotic Genetics
Group, Biotechnology Research Inst., National Research Council, 6100 Royalmount, Montreal, H4P 2R2 Quebec, Canada. Tel.: 514-496-6145; Fax:
514-496-6213; E-mail: andre.nantel@nrc.ca.
§
Recipient of a post-doctoral fellowship from the Medical Research
Council of Canada.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Kebache, J. Ash, M. G. Annis, J. Hagan, M. Huber, J. Hassard, C. L. Stewart, M. Whiteway, and A. Nantel Grb10 and Active Raf-1 Kinase Promote Bad-dependent Cell Survival J. Biol. Chem., July 27, 2007; 282(30): 21873 - 21883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Lu, S. Ren, P. Korge, J. Choi, Y. Dong, J. Weiss, C. Koehler, J.-n. Chen, and Y. Wang A novel mitochondrial matrix serine/threonine protein phosphatase regulates the mitochondria permeability transition pore and is essential for cellular survival and development Genes & Dev., April 1, 2007; 21(7): 784 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Wang and R. J. Deschenes Plasma Membrane Localization of Ras Requires Class C Vps Proteins and Functional Mitochondria in Saccharomyces cerevisiae Mol. Cell. Biol., April 15, 2006; 26(8): 3243 - 3255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Beeram, A. Patnaik, and E. K. Rowinsky Raf: A Strategic Target for Therapeutic Development Against Cancer J. Clin. Oncol., September 20, 2005; 23(27): 6771 - 6790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Urschel, F. Bassermann, R.-Y. Bai, S. Munch, C. Peschel, and J. Duyster Phosphorylation of Grb10 Regulates Its Interaction with 14-3-3 J. Biol. Chem., April 29, 2005; 280(17): 16987 - 16993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Sridhar, D. Hedley, and L. L. Siu Raf kinase as a target for anticancer therapeutics Mol. Cancer Ther., April 1, 2005; 4(4): 677 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Boerner, M. L. Demory, C. Silva, and S. J. Parsons Phosphorylation of Y845 on the Epidermal Growth Factor Receptor Mediates Binding to the Mitochondrial Protein Cytochrome c Oxidase Subunit II Mol. Cell. Biol., August 15, 2004; 24(16): 7059 - 7071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Murdaca, C. Treins, M.-N. Monthouel-Kartmann, R. Pontier-Bres, S. Kumar, E. Van Obberghen, and S. Giorgetti-Peraldi Grb10 Prevents Nedd4-mediated Vascular Endothelial Growth Factor Receptor-2 Degradation J. Biol. Chem., June 18, 2004; 279(25): 26754 - 26761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Deng, S. Bhattacharya, O. R. Swamy, R. Tandon, Y. Wang, R. Janda, and H. Riedel Growth Factor Receptor-binding Protein 10 (Grb10) as a Partner of Phosphatidylinositol 3-Kinase in Metabolic Insulin Action J. Biol. Chem., October 10, 2003; 278(41): 39311 - 39322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Giovannone, E. Lee, L. Laviola, F. Giorgino, K. A. Cleveland, and R. J. Smith Two Novel Proteins That Are Linked to Insulin-like Growth Factor (IGF-I) Receptors by the Grb10 Adapter and Modulate IGF-I Signaling J. Biol. Chem., August 22, 2003; 278(34): 31564 - 31573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Russell and P. J. Tofilon Radiation-induced Activation of Nuclear Factor-kappa B Involves Selective Degradation of Plasma Membrane-associated Ikappa Balpha Mol. Biol. Cell, October 1, 2002; 13(10): 3431 - 3440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Dos Santos, A. J. Kowaltowski, M. N. Laclau, S. Seetharaman, P. Paucek, S. Boudina, J.-B. Thambo, L. Tariosse, and K. D. Garlid Mechanisms by which opening the mitochondrial ATP- sensitive K+ channel protects the ischemic heart Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H284 - H295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hindley and W. Kolch Extracellular signal regulated kinase (ERK)/mitogen activated protein kinase (MAPK)-independent functions of Raf kinases J. Cell Sci., April 15, 2002; 115(8): 1575 - 1581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Jahn, P. Seipel, S. Urschel, C. Peschel, and J. Duyster Role for the Adaptor Protein Grb10 in the Activation of Akt Mol. Cell. Biol., February 15, 2002; 22(4): 979 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bereziat, A. Kasus-Jacobi, D. Perdereau, B. Cariou, J. Girard, and A.-F. Burnol Inhibition of Insulin Receptor Catalytic Activity by the Molecular Adapter Grb14 J. Biol. Chem., February 8, 2002; 277(7): 4845 - 4852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pearson, F. Robinson, T. Beers Gibson, B.-e Xu, M. Karandikar, K. Berman, and M. H. Cobb Mitogen-Activated Protein (MAP) Kinase Pathways: Regulation and Physiological Functions Endocr. Rev., April 1, 2001; 22(2): 153 - 183. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Navarro and R. Baserga Limited Redundancy of Survival Signals from the Type 1 Insulin-Like Growth Factor Receptor Endocrinology, March 1, 2001; 142(3): 1073 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Peruzzi, M. Prisco, A. Morrione, B. Valentinis, and R. Baserga Anti-apoptotic Signaling of the Insulin-like Growth Factor-I Receptor through Mitochondrial Translocation of c-Raf and Nedd4 J. Biol. Chem., July 6, 2001; 276(28): 25990 - 25996. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
