JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hofmann, M. W.
Right arrow Articles by Bakke, O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hofmann, M. W.
Right arrow Articles by Bakke, O.

J Biol Chem, Vol. 274, Issue 51, 36153-36158, December 17, 1999

The Leucine-based Sorting Motifs in the Cytoplasmic Domain of the Invariant Chain Are Recognized by the Clathrin Adaptors AP1 and AP2 and their Medium Chains*

Michael W. HofmannDagger §, Stefan Höning§, Dmitrii Rodionov||, Bernhard DobbersteinDagger , Kurt von Figura, and Oddmund Bakke||**

From the Dagger  Center for Molecular Biology, University of Heidelberg, 69120 Heidelberg, Germany,  Institute for Biochemistry II, University of Göttingen, 37073 Göttingen, Germany, and || Department of Biology, University of Oslo, N-0316 Oslo, Norway

Recognition of sorting signals within the cytoplasmic tail of membrane proteins by adaptor protein complexes is a crucial step in membrane protein sorting. The three known adaptor complexes, AP1, AP2, and AP3, have all been shown to recognize tyrosine- and leucine-based sorting signals, which are the most common sorting signals within membrane protein cytoplasmic tails. Although tyrosine-based signals are recognized by the µ-chains of adaptor complexes, the subunit recognizing leucine-based sorting signals is less clear.

In this report we show by surface plasmon resonance that the two leucine-based sorting signals within the cytoplasmic tail of the invariant chain bind independently from each other to AP1 and AP2 but not to AP3. We also show that both motifs can be recognized by the µ-chains of AP1 and AP2. Moreover, by using monomeric as well as trimeric invariant chain constructs, we show that adaptor binding does not require trimerization of the invariant chain.


* This work was supported by German Science Foundation Grant SFB523, by the Norwegian Cancer Society, and by European Community Grant XCT960058 (to K. v. F. and O. B.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ The first two authors contributed equally to this work.

** To whom correspondence should be addressed: Dept. of Biology, University of Oslo, Box 1050 Blindern, N-0316 Oslo, Norway. Tel.: 47 22855787; Fax: 47 22854605; E-mail: oddmund.bakke@bio.uio.no.


Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.



This article has been cited by other articles:


Home page
J. Biol. Chem.Home page
A. K. Mason, B. E. Jacobs, and P. A. Welling
AP-2-dependent Internalization of Potassium Channel Kir2.3 Is Driven by a Novel Di-hydrophobic Signal
J. Biol. Chem., March 7, 2008; 283(10): 5973 - 5984.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
A. C. Berger, G. Salazar, M. L. Styers, K. A. Newell-Litwa, E. Werner, R. A. Maue, A. H. Corbett, and V. Faundez
The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3
J. Cell Sci., October 15, 2007; 120(20): 3640 - 3652.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
B. Doray, I. Lee, J. Knisely, G. Bu, and S. Kornfeld
The {gamma}/{sigma}1 and {alpha}/{sigma}2 Hemicomplexes of Clathrin Adaptors AP-1 and AP-2 Harbor the Dileucine Recognition Site
Mol. Biol. Cell, May 1, 2007; 18(5): 1887 - 1896.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
U. Schmidt, S. Briese, K. Leicht, A. Schurmann, H.-G. Joost, and H. Al-Hasani
Endocytosis of the glucose transporter GLUT8 is mediated by interaction of a dileucine motif with the {beta}2-adaptin subunit of the AP-2 adaptor complex
J. Cell Sci., June 1, 2006; 119(11): 2321 - 2331.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
P. J. McCormick, J. A. Martina, and J. S. Bonifacino
Involvement of clathrin and AP-2 in the trafficking of MHC class II molecules to antigen-processing compartments
PNAS, May 31, 2005; 102(22): 7910 - 7915.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Dugast, H. Toussaint, C. Dousset, and P. Benaroch
AP2 Clathrin Adaptor Complex, but Not AP1, Controls the Access of the Major Histocompatibility Complex (MHC) Class II to Endosomes
J. Biol. Chem., May 20, 2005; 280(20): 19656 - 19664.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
S. H. Coleman, N. Van Damme, J. R. Day, C. M. Noviello, D. Hitchin, R. Madrid, S. Benichou, and J. C. Guatelli
Leucine-Specific, Functional Interactions between Human Immunodeficiency Virus Type 1 Nef and Adaptor Protein Complexes
J. Virol., February 15, 2005; 79(4): 2066 - 2078.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Storch, S. Pohl, and T. Braulke
A Dileucine Motif and a Cluster of Acidic Amino Acids in the Second Cytoplasmic Domain of the Batten Disease-related CLN3 Protein Are Required for Efficient Lysosomal Targeting
J. Biol. Chem., December 17, 2004; 279(51): 53625 - 53634.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M.-H. Kim and L. B. Hersh
The Vesicular Acetylcholine Transporter Interacts with Clathrin-associated Adaptor Complexes AP-1 and AP-2
J. Biol. Chem., March 26, 2004; 279(13): 12580 - 12587.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Biol.Home page
K. Janvier, Y. Kato, M. Boehm, J. R. Rose, J. A. Martina, B.-Y. Kim, S. Venkatesan, and J. S. Bonifacino
Recognition of dileucine-based sorting signals from HIV-1 Nef and LIMP-II by the AP-1 {gamma}-{sigma}1 and AP-3 {delta}-{sigma}3 hemicomplexes
J. Cell Biol., December 22, 2003; 163(6): 1281 - 1290.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
S. A. Rajasekaran, G. Anilkumar, E. Oshima, J. U. Bowie, H. Liu, W. Heston, N. H. Bander, and A. K. Rajasekaran
A Novel Cytoplasmic Tail MXXXL Motif Mediates the Internalization of Prostate-specific Membrane Antigen
Mol. Biol. Cell, December 1, 2003; 14(12): 4835 - 4845.
[Abstract] [Full Text] [PDF]


Home page
J. Exp. Med.Home page
D. Elewaut, A. P. Lawton, N. A. Nagarajan, E. Maverakis, A. Khurana, S. Honing, C. A. Benedict, E. Sercarz, O. Bakke, M. Kronenberg, et al.
The Adaptor Protein AP-3 Is Required for CD1d-Mediated Antigen Presentation of Glycosphingolipids and Development of V{alpha}14i NKT Cells
J. Exp. Med., October 20, 2003; 198(8): 1133 - 1146.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. G. Rodionov, S. Honing, A. Silye, T. L. Kongsvik, K. von Figura, and O. Bakke
Structural Requirements for Interactions between Leucine-sorting Signals and Clathrin-associated Adaptor Protein Complex AP3
J. Biol. Chem., November 27, 2002; 277(49): 47436 - 47443.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
J.-C. Lu, P. Scott, G. J. Strous, and L. A. Schuler
Multiple Internalization Motifs Differentially Used by Prolactin Receptor Isoforms Mediate Similar Endocytic Pathways
Mol. Endocrinol., November 1, 2002; 16(11): 2515 - 2527.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
D. Yao, M. Ehrlich, Y. I. Henis, and E. B. Leof
Transforming Growth Factor-beta Receptors Interact with AP2 by Direct Binding to beta 2 Subunit
Mol. Biol. Cell, November 1, 2002; 13(11): 4001 - 4012.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
H. Sugimoto, M. Sugahara, H. Folsch, Y. Koide, F. Nakatsu, N. Tanaka, T. Nishimura, M. Furukawa, C. Mullins, N. Nakamura, et al.
Differential Recognition of Tyrosine-based Basolateral Signals by AP-1B Subunit {micro}1B in Polarized Epithelial Cells
Mol. Biol. Cell, July 1, 2002; 13(7): 2374 - 2382.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
M. Geyer, O. T. Fackler, and B. M. Peterlin
Subunit H of the V-ATPase Involved in Endocytosis Shows Homology to beta -Adaptins
Mol. Biol. Cell, June 1, 2002; 13(6): 2045 - 2056.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
T. L. Kongsvik, S. Honing, O. Bakke, and D. G. Rodionov
Mechanism of Interaction between Leucine-based Sorting Signals from the Invariant Chain and Clathrin-associated Adaptor Protein Complexes AP1 and AP2
J. Biol. Chem., May 3, 2002; 277(19): 16484 - 16488.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. Lagaudriere-Gesbert, S. L. Newmyer, T. F. Gregers, O. Bakke, and H. L. Ploegh
Uncoating ATPase Hsc70 is recruited by invariant chain and controls the size of endocytic compartments
PNAS, January 24, 2002; (2002) 42688099.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
V. Bello, J. W. Goding, V. Greengrass, A. Sali, V. Dubljevic, C. Lenoir, G. Trugnan, and M. Maurice
Characterization of a Di-leucine-based Signal in the Cytoplasmic Tail of the Nucleotide-pyrophosphatase NPP1 That Mediates Basolateral Targeting but not Endocytosis
Mol. Biol. Cell, October 1, 2001; 12(10): 3004 - 3015.
[Abstract] [Full Text] [PDF]


Home page
Mol. Biol. CellHome page
J. Shim, P. W. Sternberg, and J. Lee
Distinct and Redundant Functions of {micro}1 Medium Chains of the AP-1 Clathrin-Associated Protein Complex in the Nematode Caenorhabditis elegans
Mol. Biol. Cell, August 1, 2000; 11(8): 2743 - 2756.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
D. G. Rodionov, T. W. Nordeng, T. L. Kongsvik, and O. Bakke
The Cytoplasmic Tail of CD1d Contains Two Overlapping Basolateral Sorting Signals
J. Biol. Chem., March 17, 2000; 275(12): 8279 - 8282.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. A. Laporte, R. H. Oakley, J. A. Holt, L. S. Barak, and M. G. Caron
The Interaction of beta -Arrestin with the AP-2 Adaptor Is Required for the Clustering of beta 2-Adrenergic Receptor into Clathrin-coated Pits
J. Biol. Chem., July 21, 2000; 275(30): 23120 - 23126.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. Brunet, A. Samaan, F. Deshaies, T. J. Kindt, and J. Thibodeau
Functional Characterization of a Lysosomal Sorting Motif in the Cytoplasmic Tail of HLA-DObeta
J. Biol. Chem., November 17, 2000; 275(47): 37062 - 37071.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Storch and T. Braulke
Multiple C-terminal Motifs of the 46-kDa Mannose 6-Phosphate Receptor Tail Contribute to Efficient Binding of Medium Chains of AP-2 and AP-3
J. Biol. Chem., February 2, 2001; 276(6): 4298 - 4303.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. Fingerhut, K. von Figura, and S. Honing
Binding of AP2 to Sorting Signals Is Modulated by AP2 Phosphorylation
J. Biol. Chem., February 16, 2001; 276(8): 5476 - 5482.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. Lagaudriere-Gesbert, S. L. Newmyer, T. F. Gregers, O. Bakke, and H. L. Ploegh
Uncoating ATPase Hsc70 is recruited by invariant chain and controls the size of endocytic compartments
PNAS, February 5, 2002; 99(3): 1515 - 1520.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.