| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 51, 36300-36304, December 17, 1999
§,,,
From the The primary sequence of the murine fatty acid
transport protein (FATP1) is very similar to the multigene family of
very long chain (C20-C26) acyl-CoA synthetases. To determine if FATP1
is a long chain acyl coenzyme A synthetase, FATP1-Myc/His fusion protein was expressed in COS1 cells, and its enzymatic activity was
analyzed. In addition, mutations were generated in two domains conserved in acyl-CoA synthetases: a 6- amino acid substitution into
the putative active site (amino acids 249-254) generating mutant M1
and a 59-amino acid deletion into a conserved C-terminal domain (amino
acids 464-523) generating mutant M2. Immunolocalization revealed that
the FATP1-Myc/His forms were distributed between the COS1 cell plasma
membrane and intracellular membranes. COS1 cells expressing wild type
FATP1-Myc/His exhibited a 3-fold increase in the ratio of
lignoceroyl-CoA synthetase activity (C24:0) to palmitoyl-CoA synthetase
activity (C16:0), characteristic of very long chain acyl-CoA
synthetases, whereas both mutant M1 and M2 were catalytically inactive.
Detergent-solubilized FATP1-Myc/His was partially purified using
nickel-based affinity chromatography and demonstrated a 10-fold
increase in very long chain acyl-CoA specific activity (C24:0/C16:0).
These results indicate that FATP1 is a very long chain acyl-CoA
synthetase and suggest that a potential mechanism for facilitating
mammalian fatty acid uptake is via esterification coupled influx.
Department of Biochemistry, Molecular
Biology and Biophysics, University of Minnesota, St. Paul, Minnesota
55108 and the ¶ Kennedy Krieger Research Institute,
Baltimore, Maryland 21205
To whom correspondence should be addressed: Dept. of
Biochemistry, Molecular Biology and Biophysics, University of
Minnesota, 1479 Gortner Ave., St. Paul, MN 55108. Tel.: 612-624-2712;
Fax: 612-625-5780; E-mail: david-b@biosci.cbs.umn.edu.
This article has been cited by other articles:
|
|
 |
|
  M. E. Rinella, M. S. Elias, R. R. Smolak, T. Fu, J. Borensztajn, and R. M. Green Mechanisms of hepatic steatosis in mice fed a lipogenic methionine choline-deficient diet J. Lipid Res., May 1, 2008; 49(5): 1068 - 1076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Li, P. N. Black, A. Chokshi, A. Sandoval-Alvarez, R. Vatsyayan, W. Sealls, and C. C. DiRusso High-throughput screening for fatty acid uptake inhibitors in humanized yeast identifies atypical antipsychotic drugs that cause dyslipidemias J. Lipid Res., January 1, 2008; 49(1): 230 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Jia, C. L. Moulson, Z. Pei, J. H. Miner, and P. A. Watkins Fatty Acid Transport Protein 4 Is the Principal Very Long Chain Fatty Acyl-CoA Synthetase in Skin Fibroblasts J. Biol. Chem., July 13, 2007; 282(28): 20573 - 20583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Iakoubov, A. Izzo, A. Yeung, C. I. Whiteside, and P. L. Brubaker Protein Kinase C{zeta} Is Required for Oleic Acid-Induced Secretion of Glucagon-Like Peptide-1 by Intestinal Endocrine L Cells Endocrinology, March 1, 2007; 148(3): 1089 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bonen, A. Chabowski, J. J. F. P Luiken, and J. F. C. Glatz Mechanisms and Regulation of Protein-Mediated Cellular Fatty Acid Uptake: Molecular, Biochemical, and Physiological Evidence Physiology, February 1, 2007; 22(1): 15 - 28. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Milger, T. Herrmann, C. Becker, D. Gotthardt, J. Zickwolf, R. Ehehalt, P. A. Watkins, W. Stremmel, and J. Fullekrug Cellular uptake of fatty acids driven by the ER-localized acyl-CoA synthetase FATP4 J. Cell Sci., November 15, 2006; 119(22): 4678 - 4688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Larque, S. Krauss-Etschmann, C. Campoy, D. Hartl, J. Linde, M. Klingler, H. Demmelmair, A. Cano, A. Gil, B. Bondy, et al. Docosahexaenoic acid supply in pregnancy affects placental expression of fatty acid transport proteins. Am. J. Clinical Nutrition, October 1, 2006; 84(4): 853 - 861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. An and B. Rodrigues Role of changes in cardiac metabolism in development of diabetic cardiomyopathy Am J Physiol Heart Circ Physiol, October 1, 2006; 291(4): H1489 - H1506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Doege and A. Stahl Protein-mediated Fatty Acid uptake: novel insights from in vivo models. Physiology, August 1, 2006; 21: 259 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Wu, A. M. Ortegon, B. Tsang, H. Doege, K. R. Feingold, and A. Stahl FATP1 Is an Insulin-Sensitive Fatty Acid Transporter Involved in Diet-Induced Obesity. Mol. Cell. Biol., May 1, 2006; 26(9): 3455 - 3467. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Richards, J. D. Harp, D. S. Ory, and J. E. Schaffer Fatty acid transport protein 1 and long-chain acyl coenzyme A synthetase 1 interact in adipocytes J. Lipid Res., March 1, 2006; 47(3): 665 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kiens Skeletal Muscle Lipid Metabolism in Exercise and Insulin Resistance Physiol Rev, January 1, 2006; 86(1): 205 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. M. Coort, W. A. Coumans, A. Bonen, G. J. van der Vusse, J. F. C. Glatz, and J. J. F. P. Luiken Divergent effects of rosiglitazone on protein-mediated fatty acid uptake in adipose and in muscle tissues of Zucker rats J. Lipid Res., June 1, 2005; 46(6): 1295 - 1302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. DiRusso, H. Li, D. Darwis, P. A. Watkins, J. Berger, and P. N. Black Comparative Biochemical Studies of the Murine Fatty Acid Transport Proteins (FATP) Expressed in Yeast J. Biol. Chem., April 29, 2005; 280(17): 16829 - 16837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Hall, B. M. Wiczer, T. Herrmann, W. Stremmel, and D. A. Bernlohr Enzymatic Properties of Purified Murine Fatty Acid Transport Protein 4 and Analysis of Acyl-CoA Synthetase Activities in Tissues from FATP4 Null Mice J. Biol. Chem., March 25, 2005; 280(12): 11948 - 11954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Marszalek, C. Kitidis, C. C. DiRusso, and H. F. Lodish Long-chain Acyl-CoA Synthetase 6 Preferentially Promotes DHA Metabolism J. Biol. Chem., March 18, 2005; 280(11): 10817 - 10826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Pei, P. Fraisl, J. Berger, Z. Jia, S. Forss-Petter, and P. A. Watkins Mouse Very Long-chain Acyl-CoA Synthetase 3/Fatty Acid Transport Protein 3 Catalyzes Fatty Acid Activation but Not Fatty Acid Transport in MA-10 Cells J. Biol. Chem., December 24, 2004; 279(52): 54454 - 54462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. W. Castellani, P. Gargalovic, M. Febbraio, S. Charugundla, M.-L. Jien, and A. J. Lusis Mechanisms mediating insulin resistance in transgenic mice overexpressing mouse apolipoprotein A-II J. Lipid Res., December 1, 2004; 45(12): 2377 - 2387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. DiRusso and P. N. Black Bacterial Long Chain Fatty Acid Transport: Gateway to a Fatty Acid-responsive Signaling System J. Biol. Chem., November 26, 2004; 279(48): 49563 - 49566. [Full Text] [PDF]
|
|
|
|
|
|
D. G. Mashek, K. E. Bornfeldt, R. A. Coleman, J. Berger, D. A. Bernlohr, P. Black, C. C. DiRusso, S. A. Farber, W. Guo, N. Hashimoto, et al. Revised nomenclature for the mammalian long-chain acyl-CoA synthetase gene family J. Lipid Res., October 1, 2004; 45(10): 1958 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Sauer, R. T. Dauchy, D. E. Blask, J. A. Krause, L. K. Davidson, E. M. Dauchy, K. J. Welham, and K. Coupland Conjugated Linoleic Acid Isomers and Trans Fatty Acids Inhibit Fatty Acid Transport in Hepatoma 7288CTC and Inguinal Fat Pads in Buffalo Rats J. Nutr., August 1, 2004; 134(8): 1989 - 1997. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Y. Koonen, C. R. Benton, Y. Arumugam, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Different mechanisms can alter fatty acid transport when muscle contractile activity is chronically altered Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E1042 - E1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. K. Koo, J. B. Ohlrogge, and M. Pollard On the Export of Fatty Acids from the Chloroplast J. Biol. Chem., April 16, 2004; 279(16): 16101 - 16110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gertow, M. Bellanda, P. Eriksson, S. Boquist, A. Hamsten, M. Sunnerhagen, and R. M. Fisher Genetic and Structural Evaluation of Fatty Acid Transport Protein-4 in Relation to Markers of the Insulin Resistance Syndrome J. Clin. Endocrinol. Metab., January 1, 2004; 89(1): 392 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Gimeno, D. J. Hirsch, S. Punreddy, Y. Sun, A. M. Ortegon, H. Wu, T. Daniels, A. Stricker-Krongrad, H. F. Lodish, and A. Stahl Targeted Deletion of Fatty Acid Transport Protein-4 Results in Early Embryonic Lethality J. Biol. Chem., December 5, 2003; 278(49): 49512 - 49516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Pei, N. A. Oey, M. M. Zuidervaart, Z. Jia, Y. Li, S. J. Steinberg, K. D. Smith, and P. A. Watkins The Acyl-CoA Synthetase "Bubblegum" (Lipidosin): FURTHER CHARACTERIZATION AND ROLE IN NEURONAL FATTY ACID {beta}-OXIDATION J. Biol. Chem., November 21, 2003; 278(47): 47070 - 47078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Hall, A. J. Smith, and D. A. Bernlohr Characterization of the Acyl-CoA Synthetase Activity of Purified Murine Fatty Acid Transport Protein 1 J. Biol. Chem., October 31, 2003; 278(44): 43008 - 43013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. N. Black and C. C. DiRusso Transmembrane Movement of Exogenous Long-Chain Fatty Acids: Proteins, Enzymes, and Vectorial Esterification Microbiol. Mol. Biol. Rev., September 1, 2003; 67(3): 454 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Herrmann, F. van der Hoeven, H.-J. Grone, A. F. Stewart, L. Langbein, I. Kaiser, G. Liebisch, I. Gosch, F. Buchkremer, W. Drobnik, et al. Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy J. Cell Biol., June 23, 2003; 161(6): 1105 - 1115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Heinzer, P. A. Watkins, J.-F. Lu, S. Kemp, A. B. Moser, Y. Y. Li, S. Mihalik, J. M. Powers, and K. D. Smith A very long-chain acyl-CoA synthetase-deficient mouse and its relevance to X-linked adrenoleukodystrophy Hum. Mol. Genet., May 15, 2003; 12(10): 1145 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Gimeno, A. M. Ortegon, S. Patel, S. Punreddy, P. Ge, Y. Sun, H. F. Lodish, and A. Stahl Characterization of a Heart-specific Fatty Acid Transport Protein J. Biol. Chem., April 25, 2003; 278(18): 16039 - 16044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zou, F. Tong, N. J. Fargeman, C. Borsting, P. N. Black, and C. C. DiRusso Vectorial Acylation in Saccharomyces cerevisiae. Fat1p AND FATTY ACYL-CoA SYNTHETASE ARE INTERACTING COMPONENTS OF A FATTY ACID IMPORT COMPLEX J. Biol. Chem., April 25, 2003; 278(18): 16414 - 16422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Richards, L. L. Listenberger, A. A. Kelly, S. E. Lewis, D. S. Ory, and J. E. Schaffer Oligomerization of the Murine Fatty Acid Transport Protein 1 J. Biol. Chem., March 14, 2003; 278(12): 10477 - 10483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Kamp, W. Guo, R. Souto, P. F. Pilch, B. E. Corkey, and J. A. Hamilton Rapid Flip-flop of Oleic Acid across the Plasma Membrane of Adipocytes J. Biol. Chem., February 28, 2003; 278(10): 7988 - 7995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Hatch, A. J. Smith, F. Y. Xu, A. M. Hall, and D. A. Bernlohr FATP1 channels exogenous FA into 1,2,3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells J. Lipid Res., September 1, 2002; 43(9): 1380 - 1389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. F. P. Luiken, Y. Arumugam, R. C. Bell, J. Calles-Escandon, N. N. Tandon, J. F. C. Glatz, and A. Bonen Changes in fatty acid transport and transporters are related to the severity of insulin deficiency Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E612 - E621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Heinzer, S. Kemp, J.-F. Lu, P. A. Watkins, and K. D. Smith Mouse Very Long-chain Acyl-CoA Synthetase in X-linked Adrenoleukodystrophy J. Biol. Chem., August 2, 2002; 277(32): 28765 - 28773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-K. Choi, J. Ho, S. Curry, D. Qin, R. Bittman, and J. A. Hamilton Interactions of very long-chain saturated fatty acids with serum albumin J. Lipid Res., July 1, 2002; 43(7): 1000 - 1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Steinberg, D. J. Dyck, J. Calles-Escandon, N. N. Tandon, J. J. F. P. Luiken, J. F. C. Glatz, and A. Bonen Chronic Leptin Administration Decreases Fatty Acid Uptake and Fatty Acid Transporters in Rat Skeletal Muscle J. Biol. Chem., March 8, 2002; 277(11): 8854 - 8860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Souza, K. V. Muliro, L. Liscum, P. Lien, M. T. Yamamoto, J. E. Schaffer, G. E. Dallal, X. Wang, F. B. Kraemer, M. Obin, et al. Modulation of Hormone-sensitive Lipase and Protein Kinase A-mediated Lipolysis by Perilipin A in an Adenoviral Reconstituted System J. Biol. Chem., March 1, 2002; 277(10): 8267 - 8272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and S. J. Gould PEX11 promotes peroxisome division independently of peroxisome metabolism J. Cell Biol., February 18, 2002; 156(4): 643 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Schaffer Fatty acid transport: the roads taken Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E239 - E246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. F. P. Luiken, D. J. Dyck, X.-X. Han, N. N. Tandon, Y. Arumugam, J. F. C. Glatz, and A. Bonen Insulin induces the translocation of the fatty acid transporter FAT/CD36 to the plasma membrane Am J Physiol Endocrinol Metab, February 1, 2002; 282(2): E491 - E495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zalatan, J. A. Krause, and D. E. Blask Inhibition of Isoproterenol-Induced Lipolysis in Rat Inguinal Adipocytes in Vitro by Physiological Melatonin via a Receptor-Mediated Mechanism Endocrinology, September 1, 2001; 142(9): 3783 - 3790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Caserta, T. Tchkonia, V. N. Civelek, M. Prentki, N. F. Brown, J. D. McGarry, R. A. Forse, B. E. Corkey, J. A. Hamilton, and J. L. Kirkland Fat depot origin affects fatty acid handling in cultured rat and human preadipocytes Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E238 - E247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Sauer, R. T. Dauchy, and D. E. Blask Mechanism for the Antitumor and Anticachectic Effects of n-3 Fatty Acids Cancer Res., September 1, 2000; 60(18): 5289 - 5295. [Abstract] [Full Text]
|
|
|
|
|
|
S. J. Steinberg, J. Morgenthaler, A. K. Heinzer, K. D. Smith, and P. A. Watkins Very Long-chain Acyl-CoA Synthetases. HUMAN "BUBBLEGUM" REPRESENTS A NEW FAMILY OF PROTEINS CAPABLE OF ACTIVATING VERY LONG-CHAIN FATTY ACIDS J. Biol. Chem., November 3, 2000; 275(45): 35162 - 35169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. N. Black, C. C. DiRusso, D. Sherin, R. MacColl, J. Knudsen, and J. D. Weimar Affinity Labeling Fatty Acyl-CoA Synthetase with 9-p-Azidophenoxy Nonanoic Acid and the Identification of the Fatty Acid-binding Site J. Biol. Chem., December 1, 2000; 275(49): 38547 - 38553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. F. P. Luiken, Y. Arumugam, D. J. Dyck, R. C. Bell, M. M. L. Pelsers, L. P. Turcotte, N. N. Tandon, J. F. C. Glatz, and A. Bonen Increased Rates of Fatty Acid Uptake and Plasmalemmal Fatty Acid Transporters in Obese Zucker Rats J. Biol. Chem., October 26, 2001; 276(44): 40567 - 40573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Lewis, L. L. Listenberger, D. S. Ory, and J. E. Schaffer Membrane Topology of the Murine Fatty Acid Transport Protein 1 J. Biol. Chem., September 28, 2001; 276(40): 37042 - 37050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and S. J. Gould PEX11 promotes peroxisome division independently of peroxisome metabolism J. Cell Biol., February 18, 2002; 156(4): 643 - 651. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
