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J Biol Chem, Vol. 274, Issue 51, 36592-36600, December 17, 1999
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From Starting with computational tools that search for
tissue-selective expression of assembled expressed sequenced tags, we
have identified by focusing on heart libraries a novel small stress protein of 170 amino acids that we named cvHsp. cvHsp was
found as being computationally selectively and highly (0.3% of the
total RNA) expressed in human heart. The cvHsp gene mapped
to 1p36.23-p34.3 between markers D1S434 and
D1S507. The expression of cvHsp was analyzed
with RNA dot, Northern blots, or reverse transcription-polymerase chain
reaction: expression was high in heart, medium in skeletal muscle, and
low in aorta or adipose tissues. In the heart of rat models of cardiac
pathologies, cvHsp mRNA expression was either unchanged
(spontaneous hypertension), up-regulated (right ventricular hypertrophy
induced by monocrotaline treatment), or down-regulated (left
ventricular hypertrophy following aortic banding). In obese Zucker
rats, cvHsp mRNA was increased in skeletal muscle,
brown, and white adipose tissues but remained unchanged in the heart. Western blot analysis using antipeptide polyclonal antibodies revealed
two specific bands at 23 and 25 kDa for cvHsp in human heart. cvHsp
interacted in both yeast two-hybrid and immunoprecipitation experiments
with
SmithKline Beecham Laboratoires Pharmaceutiques,
4 rue du Chesnay-Beauregard, BP 58, 35762 Saint-Grégoire,
France, the
CNRS Unité Propre 041, Rennes, France,
** SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, CM19
5AD Harlow, United Kingdom, and 
SmithKline
Beecham Pharmaceuticals,
King of Prussia, Pennsylvania 19406-0939
-filamin or actin-binding protein 280. Within cvHsp, amino acid
residues 56-119 were shown to be important for its specific
interaction with the C-terminal tail of
-filamin.
This article is dedicated to the memory of Dr. Bertrand Le Douarin.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF155908 (human), AF155909 (mouse), and AF155910 (rat).
§ To whom correspondence should be addressed. Tel.: 33-299-280-04-40; Fax: 33-299-280-04-44; E-mail: Stephane_Krief@sbphrd.com. ¶ Recipient of a SmithKline Beecham/CNRS postdoctoral fellowship.
Deceased.
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