Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yokota, S.-i.
Right arrow Articles by Kubota, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yokota, S.-i.
Right arrow Articles by Kubota, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 52, 37070-37078, December 24, 1999

Cytosolic Chaperonin Is Up-regulated during Cell Growth
PREFERENTIAL EXPRESSION AND BINDING TO TUBULIN AT G1/S TRANSITION THROUGH EARLY S PHASE*

Shin-ichi Yokota, Hideki Yanagi, Takashi Yura, and Hiroshi KubotaDagger

From the HSP Research Institute, Kyoto Research Park, 17 Chudoji Minami-machi, Shimogyo-ku, Kyoto 600-8813, Japan

The chaperonin containing t-complex polypeptide 1 (CCT) is a heterooligomeric molecular chaperone assisting in the folding of actin, tubulin, and other cytosolic proteins. The expression levels of CCT subunits varied among seven mouse cell lines tested but showed a close correlation with growth rate. Both the CCT protein and mRNA levels in the human promyelolytic cell HL60 decreased concomitant with growth arrest during differentiation. More rapid decrease in CCT level occurred when the mouse interleukin (IL)-3-dependent myeloid DA3 cells were starved for IL-3. Readdition of IL-3 caused rapid resumption of CCT synthesis during synchronous growth: the maximum CCT protein and mRNA levels were observed at G1/S transition through early S phase. The turnover rate of CCT was nearly constant regardless of growth. Gel filtration and immunoprecipitation analyses indicated that CCT in vivo is associated with tubulin at early S phase, but not at G0/G1 phase. These results demonstrated that CCT expression is strongly up-regulated during cell growth especially from G1/S transition to early S phase and is primarily controlled at the mRNA level. CCT appears to play important roles for cell growth by assisting in the folding of tubulin and other proteins.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 81-75-315-8656; Fax: 81-75-315-8659; E-mail: kubota@hsp.co.jp.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
D. Carpi, M. Korkalainen, L. Airoldi, R. Fanelli, H. Hakansson, V. Muhonen, J. Tuukkanen, M. Viluksela, and R. Pastorelli
Dioxin-Sensitive Proteins in Differentiating Osteoblasts: Effects on Bone Formation In Vitro
Toxicol. Sci., April 1, 2009; 108(2): 330 - 343.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
S. Hirayama, Y. Yamazaki, A. Kitamura, Y. Oda, D. Morito, K. Okawa, H. Kimura, D. M. Cyr, H. Kubota, and K. Nagata
MKKS Is a Centrosome-shuttling Protein Degraded by Disease-causing Mutations via CHIP-mediated Ubiquitination
Mol. Biol. Cell, March 1, 2008; 19(3): 899 - 911.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
P. C. Stirling, M. Srayko, K. S. Takhar, A. Pozniakovsky, A. A. Hyman, and M. R. Leroux
Functional Interaction between Phosducin-like Protein 2 and Cytosolic Chaperonin Is Essential for Cytoskeletal Protein Function and Cell Cycle Progression
Mol. Biol. Cell, June 1, 2007; 18(6): 2336 - 2345.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
M Yoshioka, A Boivin, P Ye, F Labrie, and J St-Amand
Effects of dihydrotestosterone on skeletal muscle transcriptome in mice measured by serial analysis of gene expression.
J. Mol. Endocrinol., April 1, 2006; 36(2): 247 - 259.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
T. Shimizu, S.-i. Yokota, S. Takahashi, Y. Kunishima, K. Takeyama, N. Masumori, A. Takahashi, M. Matsukawa, N. Itoh, T. Tsukamoto, et al.
Membrane-Anchored CD14 Is Important for Induction of Interleukin-8 by Lipopolysaccharide and Peptidoglycan in Uroepithelial Cells
Clin. Vaccine Immunol., September 1, 2004; 11(5): 969 - 976.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S.-i. Yokota, N. Yokosawa, T. Okabayashi, T. Suzutani, S. Miura, K. Jimbow, and N. Fujii
Induction of Suppressor of Cytokine Signaling-3 by Herpes Simplex Virus Type 1 Contributes to Inhibition of the Interferon Signaling Pathway
J. Virol., June 15, 2004; 78(12): 6282 - 6286.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. E. Grossman, Y. Qian, K. Banki, and A. Perl
ZNF143 Mediates Basal and Tissue-specific Expression of Human Transaldolase
J. Biol. Chem., March 26, 2004; 279(13): 12190 - 12205.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S.-i. Yokota, N. Yokosawa, T. Kubota, T. Okabayashi, S. Arata, and N. Fujii
Suppression of Thermotolerance in Mumps Virus-infected Cells Is Caused by Lack of HSP27 Induction Contributed by STAT-1
J. Biol. Chem., October 24, 2003; 278(43): 41654 - 41660.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Bai, J. H. Xi, E. F. Wawrousek, T. P. Fleming, and U. P. Andley
Hyperproliferation and p53 Status of Lens Epithelial Cells Derived from {alpha}B-crystallin Knockout Mice
J. Biol. Chem., September 19, 2003; 278(38): 36876 - 36886.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Yamazaki, H. Kubota, M. Nozaki, and K. Nagata
Transcriptional Regulation of the Cytosolic Chaperonin {theta} Subunit Gene, Cctq, by Ets Domain Transcription Factors Elk-1, Sap-1a, and Net in the Absence of Serum Response Factor
J. Biol. Chem., August 15, 2003; 278(33): 30642 - 30651.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. H. Xi, F. Bai, and U. P. Andley
Reduced survival of lens epithelial cells in the {alpha}A-crystallin-knockout mouse
J. Cell Sci., March 15, 2003; 116(6): 1073 - 1085.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. L. Astier, R. Xu, M. Svoboda, E. Hinds, O. Munoz, R. de Beaumont, C. D. Crean, T. Gabig, and A. S. Freedman
Temporal gene expression profile of human precursor B leukemia cells induced by adhesion receptor: identification of pathways regulating B-cell survival
Blood, February 1, 2003; 101(3): 1118 - 1127.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kubota, S.-i. Yokota, H. Yanagi, and T. Yura
Transcriptional Regulation of the Mouse Cytosolic Chaperonin Subunit Gene Ccta/t-Complex Polypeptide 1 by Selenocysteine tRNA Gene Transcription Activating Factor Family Zinc Finger Proteins
J. Biol. Chem., September 8, 2000; 275(37): 28641 - 28648.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement