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J Biol Chem, Vol. 274, Issue 52, 37079-37086, December 24, 1999

The Herpes Simplex Virus Type 1 Origin-binding Protein
SEQUENCE-SPECIFIC ACTIVATION OF ADENOSINE TRIPHOSPHATASE ACTIVITY BY A DOUBLE-STRANDED DNA CONTAINING BOX I*

Lauren B. Murata and Mark S. DodsonDagger

From the Department of Biochemistry, University of Arizona, Tucson, Arizona 85721-0088

Origin-dependent replication of the herpes simplex virus type 1 genome requires the virally encoded origin-binding protein, UL9. UL9 binds specifically to the herpes simplex virus type 1 replication origin at two high affinity binding sites on the DNA, Boxes I and II. UL9 also has ATP-dependent DNA helicase and DNA-stimulated ATPase activities that are used to unwind the origin DNA. Origin-specific binding is mediated by the C-terminal domain (C-domain) of the enzyme. ATPase and helicase activities are mediated by the N-terminal domain (N-domain). Previous studies have shown that single-stranded DNA is a good coeffector for ATPase activity. We have analyzed several DNAs for their ability to stimulate the ATPase activity of UL9 and of a truncated UL9 protein (UL9/N) consisting only of the N-domain. We report here that duplex Box I DNA specifically and potently stimulates the ATPase activity of UL9 but not of UL9/N. We also find that removal of the C-domain significantly increases the ATPase activity of UL9. We have incorporated these results into a model for initiation in which the C-domain of UL9 serves to regulate the enzymatic activity of the N-domain.

* This research was supported by Grant RPG9705601NP from the American Cancer Society.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry, Biological Sciences West Bldg., 1041 E. Lowell St., University of Arizona, Tucson, AZ 85721-0088. Tel.: 520-621-6123; Fax: 520-621-9288; E-mail: dodson@u.arizona.edu.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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