|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 53, 37517-37524, December 31, 1999
From the MerR-like DNA distortion mechanisms have been
proposed for a variety of stress-responsive transcription factors. The
Escherichia coli ZntR protein, a homologue of MerR, has
recently been shown to mediate Zn(II)-responsive regulation of
zntA, a gene involved in Zn(II) detoxification. To
determine whether the MerR DNA distortion mechanism is conserved among
MerR family members, we have purified ZntR to homogeneity and shown
that it is a zinc receptor that is necessary and sufficient to
stimulate Zn-responsive transcription at the zntA promoter.
Biochemical, DNA footprinting, and in vitro transcription
assays indicate that apo-ZntR binds in the atypical 20-base pair spacer
region of the promoter and distorts the DNA in a manner that is similar
to apo-MerR. The addition of Zn(II) to ZntR converts it to a
transcriptional activator protein that introduces changes in the DNA
conformation. These changes apparently make the promoter a better
substrate for RNA polymerase. We propose that this zinc-sensing
homologue of MerR restructures the target promoter in a manner similar
to that of other stress-responsive transcription factors. The ZntR
metalloregulatory protein is a direct Zn(II) sensor that catalyzes
transcriptional activation of a zinc efflux gene, thus preventing
intracellular Zn(II) from exceeding an optimal but as yet unknown concentration.
DNA Distortion Mechanism for Transcriptional Activation by ZntR,
a Zn(II)-responsive MerR Homologue in Escherichia coli*
,§¶
Department of Chemistry and the
§ Department of Biochemistry, Molecular Biology, and Cell
Biology, Northwestern University, Evanston, Illinois 60208
*
This work was supported in part by National Institutes of
Health Grants R01 GM38784 (to T. V. O.), and T32 GM08382 (to
C. E. O.), and T32 GM08061 (to F. W. O.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Collado-Vides, H. Salgado, E. Morett, S. Gama-Castro, V. Jimenez-Jacinto, I. Martinez-Flores, A. Medina-Rivera, L. Muniz-Rascado, M. Peralta-Gil, and A. Santos-Zavaleta Bioinformatics Resources for the Study of Gene Regulation in Bacteria J. Bacteriol., January 1, 2009; 191(1): 23 - 31. [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Newberry, J. L. Huffman, M. C. Miller, N. Vazquez-Laslop, A. A. Neyfakh, and R. G. Brennan Structures of BmrR-Drug Complexes Reveal a Rigid Multidrug Binding Pocket and Transcription Activation through Tyrosine Expulsion J. Biol. Chem., September 26, 2008; 283(39): 26795 - 26804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Helbig, C. Bleuel, G. J. Krauss, and D. H. Nies Glutathione and Transition-Metal Homeostasis in Escherichia coli J. Bacteriol., August 1, 2008; 190(15): 5431 - 5438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Watanabe, A. Kita, K. Kobayashi, and K. Miki Crystal structure of the [2Fe-2S] oxidative-stress sensor SoxR bound to DNA PNAS, March 18, 2008; 105(11): 4121 - 4126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Gorodetsky, L. E. P. Dietrich, P. E. Lee, B. Demple, D. K. Newman, and J. K. Barton DNA binding shifts the redox potential of the transcription factor SoxR PNAS, March 11, 2008; 105(10): 3684 - 3689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Espariz, S. K. Checa, M. E. P. Audero, L. B. Pontel, and F. C. Soncini Dissecting the Salmonella response to copper Microbiology, September 1, 2007; 153(9): 2989 - 2997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pruteanu, S. B. Neher, and T. A. Baker Ligand-Controlled Proteolysis of the Escherichia coli Transcriptional Regulator ZntR J. Bacteriol., April 15, 2007; 189(8): 3017 - 3025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Wray Jr. and S. H. Fisher Functional Analysis of the Carboxy-Terminal Region of Bacillus subtilis TnrA, a MerR Family Protein J. Bacteriol., January 1, 2007; 189(1): 20 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ventura, J. G. Kenny, Z. Zhang, G. F. Fitzgerald, and D. van Sinderen The clpB gene of Bifidobacterium breve UCC 2003: transcriptional analysis and first insights into stress induction Microbiology, September 1, 2005; 151(9): 2861 - 2872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Chander and B. Demple Functional Analysis of SoxR Residues Affecting Transduction of Oxidative Stress Signals into Gene Expression J. Biol. Chem., October 1, 2004; 279(40): 41603 - 41610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Newberry and R. G. Brennan The Structural Mechanism for Transcription Activation by MerR Family Member Multidrug Transporter Activation, N Terminus J. Biol. Chem., May 7, 2004; 279(19): 20356 - 20362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Song, J. Caguiat, Z. Li, J. Shokes, R. A. Scott, L. Olliff, and A. O. Summers Engineered Single-Chain, Antiparallel, Coiled Coil Mimics the MerR Metal Binding Site J. Bacteriol., March 15, 2004; 186(6): 1861 - 1868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gaballa, M. Cao, and J. D. Helmann Two MerR homologues that affect copper induction of the Bacillus subtilis copZA operon Microbiology, December 1, 2003; 149(12): 3413 - 3421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Godsey, E. E. Zheleznova Heldwein, and R. G. Brennan Structural Biology of Bacterial Multidrug Resistance Gene Regulators J. Biol. Chem., October 18, 2002; 277(43): 40169 - 40172. [Full Text] [PDF]
|
|
|
|
|
|
J. J. Lopez-Rubio, M. Elias-Arnanz, S. Padmanabhan, and F. J. Murillo A Repressor-Antirepressor Pair Links Two Loci Controlling Light-induced Carotenogenesis in Myxococcus xanthus J. Biol. Chem., February 22, 2002; 277(9): 7262 - 7270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Basak and V. Nagaraja DNA Unwinding Mechanism for the Transcriptional Activation of momP1 Promoter by the Transactivator Protein C of Bacteriophage Mu J. Biol. Chem., December 7, 2001; 276(50): 46941 - 46945. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Godsey, N. N. Baranova, A. A. Neyfakh, and R. G. Brennan Crystal Structure of MtaN, a Global Multidrug Transporter Gene Activator J. Biol. Chem., December 7, 2001; 276(50): 47178 - 47184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Giedroc, X. Chen, M. A. Pennella, and A. C. LiWang Conformational Heterogeneity in the C-terminal Zinc Fingers of Human MTF-1. AN NMR AND ZINC-BINDING STUDY J. Biol. Chem., November 2, 2001; 276(45): 42322 - 42332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Grass, B. Fan, B. P. Rosen, S. Franke, D. H. Nies, and C. Rensing ZitB (YbgR), a Member of the Cation Diffusion Facilitator Family, Is an Additional Zinc Transporter in Escherichia coli J. Bacteriol., August 1, 2001; 183(15): 4664 - 4667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-W. Lee, E. Glickmann, and D. A. Cooksey Chromosomal Locus for Cadmium Resistance in Pseudomonas putida Consisting of a Cadmium-Transporting ATPase and a MerR Family Response Regulator Appl. Envir. Microbiol., April 1, 2001; 67(4): 1437 - 1444. [Abstract] [Full Text]
|
|
|
|
|
|
T. A. Slieman, R. Rebeil, and W. L. Nicholson Spore Photoproduct (SP) Lyase from Bacillus subtilis Specifically Binds to and Cleaves SP (5-Thyminyl-5,6-Dihydrothymine) but Not Cyclobutane Pyrimidine Dimers in UV-Irradiated DNA J. Bacteriol., November 15, 2000; 182(22): 6412 - 6417. [Abstract] [Full Text]
|
|
|
|
|
|
F. W. Outten, C. E. Outten, J. Hale, and T. V. O'Halloran Transcriptional Activation of an Escherichia coli Copper Efflux Regulon by the Chromosomal MerR Homologue, CueR J. Biol. Chem., September 29, 2000; 275(40): 31024 - 31029. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |