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J Biol Chem, Vol. 274, Issue 53, 37932-37940, December 31, 1999
From the Section of Molecular Endocrinology, Endocrinology and
Reproduction Research Branch, NICHHD, National Institutes of Health,
Bethesda, Maryland 20892
A gonadotropin-regulated testicular RNA helicase
(GRTH) was identified and characterized. GRTH cloned from rat Leydig
cell, mouse testis, and human testis cDNA libraries is a novel
member of the DEAD-box protein family. GRTH is transcriptionally
up-regulated by chorionic gonadotropin via cyclic AMP-induced androgen
formation in the Leydig cell. It has ATPase and RNA helicase activities and increases translation in vitro. This helicase is highly
expressed in rat, mouse, and human testes and weakly expressed in the
pituitary and hypothalamus. GRTH is produced in both somatic (Leydig
cells) and germinal (meiotic spermatocytes and round haploid
spermatids) cells and is developmentally regulated. GRTH predominantly
localized in the cytoplasm may function as a translational activator.
This novel helicase could be relevant to the control of steroidogenesis and the paracrine regulation of androgen-dependent
spermatogenesis in the testis.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF142629, AF142630, AF155140.
A Novel Gonadotropin-regulated Testicular RNA Helicase
A NEW MEMBER OF THE DEAD-BOX FAMILY*
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Bldg. 49, Rm. 6A36, 49 Convent Dr. MSC4510, National Institutes of Health, Bethesda, MD
20892-4510. Tel.: 301-496-2021: Fax: 301-480-8010: E-mail: dufau@helix.nih.gov.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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