|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 53, 38232-38240, December 31, 1999
From the In animal cells, phosphoinositides are key
components of the inositol 1,4,5-trisphosphate/diacylglycerol-based
signaling pathway, but also have many other cellular functions. These
lipids are also believed to fulfill similar functions in plant cells,
although many details concerning the components of a plant
phosphoinositide system, and their regulation are still missing. Only
recently have the different phosphoinositide isomers been unambiguously identified in plant cells. Another problem that hinders the study of
the function of phosphoinositides and their derivatives, as well as the
regulation of their metabolism, in plant cells is the need for a
homogenous, easily obtainable material, from which the extraction and
purification of phospholipids is relatively easy and quantitatively
reproducible. We present here a thorough characterization of the
phospholipids purified from [32P]orthophosphate-
and myo-[2-3H]inositol-radiolabeled
Arabidopsis thaliana suspension-cultured cells. We then
show that NaCl treatment induces dramatic increases in the levels of
phosphatidylinositol 4,5-bisphosphate and diacylglycerol pyrophosphate
and also affects the turnover of phosphatidylcholine. The increase in
phosphatidylinositol 4,5-bisphosphate was also observed with a
non-ionic hyperosmotic shock. In contrast, the increase in
diacylglycerol pyrophosphate and the turnover of phosphatidylcholine were relatively specific to salt treatments as only minor changes in
the metabolism of these two phospholipids were detected when the cells
were treated with sorbitol instead of NaCl.
Salinity and Hyperosmotic Stress Induce Rapid Increases in
Phosphatidylinositol 4,5-Bisphosphate, Diacylglycerol Pyrophosphate,
and Phosphatidylcholine in Arabidopsis
thaliana Cells*
§,,
,, and
Department of Plant Biochemistry, Lund
University, P. O. Box 117, SE-22100 Lund, Sweden and the ¶ Centre
for Clinical Research in Immunology and Signalling, University of
Birmingham, Birmingham B15 2TT, United Kingdom
*
This work was supported by European Community Grant
BIO-CT96-0775 (to C. P., C. L., and M. S.) and by grants
from the Swedish Natural Science Research Council (to C. L.), the
Swedish Council for Forestry and Agricultural Research (to M. S.),
and the Swedish Foundation for Strategic Research (to C. L. and
M. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported from the Medical Research Council, Royal Society,
and Medical Faculty of the University of Birmingham; Medical Research Council research fellow.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Darwish, C. Testerink, M. Khalil, O. El-Shihy, and T. Munnik Phospholipid Signaling Responses in Salt-Stressed Rice Leaves Plant Cell Physiol., May 1, 2009; 50(5): 986 - 997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Saavedra, V. Balbi, S. K. Dove, Y. Hiwatashi, K. Mikami, and M. Sommarin Characterization of Phosphatidylinositol Phosphate Kinases from the Moss Physcomitrella patens: PpPIPK1 and PpPIPK2 Plant Cell Physiol., March 1, 2009; 50(3): 595 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mosblech, S. Konig, I. Stenzel, P. Grzeganek, I. Feussner, and I. Heilmann Phosphoinositide and Inositolpolyphosphate Signalling in Defense Responses of Arabidopsis thaliana Challenged by Mechanical Wounding Mol Plant, March 1, 2008; 1(2): 249 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Konig, A. Mosblech, and I. Heilmann Stress-inducible and constitutive phosphoinositide pools have distinctive fatty acid patterns in Arabidopsis thaliana FASEB J, July 1, 2007; 21(9): 1958 - 1967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Im, I. Y. Perera, I. Brglez, A. J. Davis, J. Stevenson-Paulik, B. Q. Phillippy, E. Johannes, N. S. Allen, and W. F. Boss Increasing Plasma Membrane Phosphatidylinositol(4,5)Bisphosphate Biosynthesis Increases Phosphoinositide Metabolism in Nicotiana tabacum PLANT CELL, May 1, 2007; 19(5): 1603 - 1616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Parre, M. A. Ghars, A.-S. Leprince, L. Thiery, D. Lefebvre, M. Bordenave, L. Richard, C. Mazars, C. Abdelly, and A. Savoure Calcium Signaling via Phospholipase C Is Essential for Proline Accumulation upon Ionic But Not Nonionic Hyperosmotic Stresses in Arabidopsis Plant Physiology, May 1, 2007; 144(1): 503 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Im, A. J. Davis, I. Y. Perera, E. Johannes, N. S. Allen, and W. F. Boss The N-terminal Membrane Occupation and Recognition Nexus Domain of Arabidopsis Phosphatidylinositol Phosphate Kinase 1 Regulates Enzyme Activity J. Biol. Chem., February 23, 2007; 282(8): 5443 - 5452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-T. Liu, Y.-Y. Liu, Q.-H. Pan, H.-R. Yang, J.-C. Zhan, and W.-D. Huang Novel interrelationship between salicylic acid, abscisic acid, and PIP2-specific phospholipase C in heat acclimation-induced thermotolerance in pea leaves J. Exp. Bot., September 1, 2006; 57(12): 3337 - 3347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zalejski, S. Paradis, R. Maldiney, Y. Habricot, E. Miginiac, J.-P. Rona, and E. Jeannette Induction of Abscisic Acid-Regulated Gene Expression by Diacylglycerol Pyrophosphate Involves Ca2+ and Anion Currents in Arabidopsis Suspension Cells Plant Physiology, August 1, 2006; 141(4): 1555 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Alvarez-Venegas, M. Sadder, A. Hlavacka, F. Baluska, Y. Xia, G. Lu, A. Firsov, G. Sarath, H. Moriyama, J. G. Dubrovsky, et al. The Arabidopsis homolog of trithorax, ATX1, binds phosphatidylinositol 5-phosphate, and the two regulate a common set of target genes PNAS, April 11, 2006; 103(15): 6049 - 6054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Williams, J. Torabinejad, E. Cohick, K. Parker, E. J. Drake, J. E. Thompson, M. Hortter, and D. B. DeWald Mutations in the Arabidopsis Phosphoinositide Phosphatase Gene SAC9 Lead to Overaccumulation of PtdIns(4,5)P2 and Constitutive Expression of the Stress-Response Pathway Plant Physiology, June 1, 2005; 138(2): 686 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong, D. H. Burk, C. J. Nairn, A. Wood-Jones, W. H. Morrison III, and Z.-H. Ye Mutation of SAC1, an Arabidopsis SAC Domain Phosphoinositide Phosphatase, Causes Alterations in Cell Morphogenesis, Cell Wall Synthesis, and Actin Organization PLANT CELL, May 1, 2005; 17(5): 1449 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong and Z.-H. Ye Molecular and Biochemical Characterization of Three WD-Repeat-Domain-containing Inositol Polyphosphate 5-Phosphatases in Arabidopsis thaliana Plant Cell Physiol., November 15, 2004; 45(11): 1720 - 1728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Ercetin and G. E. Gillaspy Molecular Characterization of an Arabidopsis Gene Encoding a Phospholipid-Specific Inositol Polyphosphate 5-Phosphatase Plant Physiology, June 1, 2004; 135(2): 938 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kobayashi, S. Yamamoto, H. Minami, Y. Kagaya, and T. Hattori Differential Activation of the Rice Sucrose Nonfermenting1-Related Protein Kinase2 Family by Hyperosmotic Stress and Abscisic Acid PLANT CELL, May 1, 2004; 16(5): 1163 - 1177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zonia and T. Munnik Osmotically Induced Cell Swelling versus Cell Shrinking Elicits Specific Changes in Phospholipid Signals in Tobacco Pollen Tubes Plant Physiology, February 1, 2004; 134(2): 813 - 823. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mazel, Y. Leshem, B. S. Tiwari, and A. Levine Induction of Salt and Osmotic Stress Tolerance by Overexpression of an Intracellular Vesicle Trafficking Protein AtRab7 (AtRabG3e) Plant Physiology, January 1, 2004; 134(1): 118 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Apone, N. Alyeshmerni, K. Wiens, D. Chalmers, M. J. Chrispeels, and G. Colucci The G-Protein-Coupled Receptor GCR1 Regulates DNA Synthesis through Activation of Phosphatidylinositol-Specific Phospholipase C Plant Physiology, October 1, 2003; 133(2): 571 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong and Z.-H. Ye The SAC Domain-Containing Protein Gene Family in Arabidopsis Plant Physiology, June 1, 2003; 132(2): 544 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sbrissa, O. C. Ikonomov, R. Deeb, and A. Shisheva Phosphatidylinositol 5-Phosphate Biosynthesis Is Linked to PIKfyve and Is Involved in Osmotic Response Pathway in Mammalian Cells J. Biol. Chem., November 27, 2002; 277(49): 47276 - 47284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ruelland, C. Cantrel, M. Gawer, J.-C. Kader, and A. Zachowski Activation of Phospholipases C and D Is an Early Response to a Cold Exposure in Arabidopsis Suspension Cells Plant Physiology, October 1, 2002; 130(2): 999 - 1007. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zonia, S. Cordeiro, J. Tupy, and J. A. Feijo Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate PLANT CELL, September 1, 2002; 14(9): 2233 - 2249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Mou, X. Wang, Z. Fu, Y. Dai, C. Han, J. Ouyang, F. Bao, Y. Hu, and J. Li Silencing of Phosphoethanolamine N-Methyltransferase Results in Temperature-Sensitive Male Sterility and Salt Hypersensitivity in Arabidopsis PLANT CELL, September 1, 2002; 14(9): 2031 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mueller-Roeber and C. Pical Inositol Phospholipid Metabolism in Arabidopsis. Characterized and Putative Isoforms of Inositol Phospholipid Kinase and Phosphoinositide-Specific Phospholipase C Plant Physiology, September 1, 2002; 130(1): 22 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Y. Perera, J. Love, I. Heilmann, W. F. Thompson, and W. F. Boss Up-Regulation of Phosphoinositide Metabolism in Tobacco Cells Constitutively Expressing the Human Type I Inositol Polyphosphate 5-Phosphatase Plant Physiology, August 1, 2002; 129(4): 1795 - 1806. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Nasuhoglu, S. Feng, Y. Mao, I. Shammat, M. Yamamato, S. Earnest, M. Lemmon, and D. W. Hilgemann Modulation of cardiac PIP2 by cardioactive hormones and other physiologically relevant interventions Am J Physiol Cell Physiol, July 1, 2002; 283(1): C223 - C234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xiong, K. S. Schumaker, and J.-K. Zhu Cell Signaling during Cold, Drought, and Salt Stress PLANT CELL, May 1, 2002; 14(90001): S165 - 183. [Full Text] [PDF]
|
|
|
|
 |
|
 D. W. Hilgemann, S. Feng, and C. Nasuhoglu The Complex and Intriguing Lives of PIP2 with Ion Channels and Transporters Sci. Signal., December 4, 2001; 2001(111): re19 - re19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Heilmann, I. Y. Perera, W. Gross, and W. F. Boss Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Levels Decrease with Time in Culture Plant Physiology, August 1, 2001; 126(4): 1507 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. DeWald, J. Torabinejad, C. A. Jones, J. C. Shope, A. R. Cangelosi, J. E. Thompson, G. D. Prestwich, and H. Hama Rapid Accumulation of Phosphatidylinositol 4,5-Bisphosphate and Inositol 1,4,5-Trisphosphate Correlates with Calcium Mobilization in Salt-Stressed Arabidopsis Plant Physiology, June 1, 2001; 126(2): 759 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Monks, K. Aghoram, P. D. Courtney, D. B. DeWald, and R. E. Dewey Hyperosmotic Stress Induces the Rapid Phosphorylation of a Soybean Phosphatidylinositol Transfer Protein Homolog through Activation of the Protein Kinases SPK1 and SPK2 PLANT CELL, May 1, 2001; 13(5): 1205 - 1219. [Abstract] [Full Text]
|
|
|
|
|
|
S. Takahashi, T. Katagiri, T. Hirayama, K. Yamaguchi-Shinozaki, and K. Shinozaki Hyperosmotic Stress Induces a Rapid and Transient Increase in Inositol 1,4,5-Trisphosphate Independent of Abscisic Acid in Arabidopsis Cell Culture Plant Cell Physiol., February 1, 2001; 42(2): 214 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Fan and R. A Neff Susceptibility of ATP-sensitive K+ channels to cell stress through mediation of phosphoinositides as examined by photoirradiation J. Physiol., December 15, 2000; 529(3): 707 - 721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oshiro, S. Rangaswamy, X. Chen, G.-S. Han, J. E. Quinn, and G. M. Carman Regulation of the DPP1-encoded Diacylglycerol Pyrophosphate (DGPP) Phosphatase by Inositol and Growth Phase. INHIBITION OF DGPP PHOSPHATASE ACTIVITY BY CDP-DIACYLGLYCEROL AND ACTIVATION OF PHOSPHATIDYLSERINE SYNTHASE ACTIVITY BY DGPP J. Biol. Chem., December 22, 2000; 275(52): 40887 - 40896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pierrugues, C. Brutesco, J. Oshiro, M. Gouy, Y. Deveaux, G. M. Carman, P. Thuriaux, and M. Kazmaier Lipid Phosphate Phosphatases in Arabidopsis. REGULATION OF THE AtLPP1 GENE IN RESPONSE TO STRESS J. Biol. Chem., June 1, 2001; 276(23): 20300 - 20308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-S. Han, C. N. Johnston, X. Chen, K. Athenstaedt, G. Daum, and G. M. Carman Regulation of the Saccharomyces cerevisiae DPP1-encoded Diacylglycerol Pyrophosphate Phosphatase by Zinc J. Biol. Chem., March 23, 2001; 276(13): 10126 - 10133. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |