| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 6, 3257-3260, February 5, 1999
,,
From the Clathrin-mediated endocytosis was shown to be
arrested in mitosis due to a block in the invagination of
clathrin-coated pits. A Xenopus mitotic phosphoprotein,
MP90, is very similar to an abundant mammalian nerve terminal protein,
epsin, which binds the Eps15 homology (EH) domain of Eps15 and the
Howard Hughes Medical Institute and
Department of Cell Biology, Yale University School of Medicine, New
Haven Connecticut 06510, the § Department of Experimental
Oncology, European Institute of Oncology, Milan 20141, Italy, and the
¶ Istituto di Microbiologia, Universitá di Bari,
Bari 70124, Italy
-adaptin subunit of the clathrin adaptor AP-2. We show here that
both rat epsin and Eps15 are mitotic phosphoproteins and that their
mitotic phosphorylation inhibits binding to the appendage domain of
-adaptin. Both epsin and Eps15, like other cytosolic components of
the synaptic vesicle endocytic machinery, undergo constitutive
phosphorylation and depolarization-dependent dephosphorylation
in nerve terminals. Furthermore, their binding to AP-2 in brain
extracts is enhanced by dephosphorylation. Epsin together with Eps15
was proposed to assist the clathrin coat in its dynamic
rearrangements during the invagination/fission reactions.
Their mitotic phosphorylation may be one of the mechanisms by which the
invagination of clathrin-coated pits is blocked in mitosis and their
stimulation-dependent dephosphorylation at synapses may
contribute to the compensatory burst of endocytosis after a secretory stimulus.
This article has been cited by other articles:
|
|
 |
|
  J. C. Bakowska, H. Jupille, P. Fatheddin, R. Puertollano, and C. Blackstone Troyer Syndrome Protein Spartin Is Mono-Ubiquitinated and Functions in EGF Receptor Trafficking Mol. Biol. Cell, May 1, 2007; 18(5): 1683 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Traub and G. L. Lukacs Decoding ubiquitin sorting signals for clathrin-dependent endocytosis by CLASPs J. Cell Sci., February 15, 2007; 120(4): 543 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F. Collawn Unlocking the Mysteries of Na+-K+-ATPase Endocytosis: Phosphorylation Is the Key. Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 1 - 2. [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub and D. Rotin Role of Ubiquitylation in Cellular Membrane Transport Physiol Rev, April 1, 2006; 86(2): 669 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Schweitzer, E. E. Burke, H. V. Goodson, and C. D'Souza-Schorey Endocytosis Resumes during Late Mitosis and Is Required for Cytokinesis J. Biol. Chem., December 16, 2005; 280(50): 41628 - 41635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Y. Lee, S. Voronov, K. Letinic, A. C. Nairn, G. Di Paolo, and P. De Camilli Regulation of the interaction between PIPKI{gamma} and talin by proline-directed protein kinases J. Cell Biol., February 28, 2005; 168(5): 789 - 799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Chen and P. De Camilli From the Cover: The association of epsin with ubiquitinated cargo along the endocytic pathway is negatively regulated by its interaction with clathrin PNAS, February 22, 2005; 102(8): 2766 - 2771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Mishra, M. J. Hawryluk, T. J. Brett, P. A. Keyel, A. L. Dupin, A. Jha, J. E. Heuser, D. H. Fremont, and L. M. Traub Dual Engagement Regulation of Protein Interactions with the AP-2 Adaptor {alpha} Appendage J. Biol. Chem., October 29, 2004; 279(44): 46191 - 46203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Lee, M. R. Wenk, Y. Kim, A. C. Nairn, and P. De Camilli Regulation of synaptojanin 1 by cyclin-dependent kinase 5 at synapses PNAS, January 13, 2004; 101(2): 546 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, S. Polo, P. P. Di Fiore, and P. V. De Camilli Rapid Ca2+-dependent decrease of protein ubiquitination at synapses PNAS, December 9, 2003; 100(25): 14908 - 14913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tomizawa, S. Sunada, Y.-F. Lu, Y. Oda, M. Kinuta, T. Ohshima, T. Saito, F.-Y. Wei, M. Matsushita, S.-T. Li, et al. Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles J. Cell Biol., November 24, 2003; 163(4): 813 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Morgan, K. Prasad, S. Jin, G. J. Augustine, and E. M. Lafer Eps15 Homology Domain-NPF Motif Interactions Regulate Clathrin Coat Assembly during Synaptic Vesicle Recycling J. Biol. Chem., August 29, 2003; 278(35): 33583 - 33592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rosse, S. L'Hoste, N. Offner, A. Picard, and J. Camonis RLIP, an Effector of the Ral GTPases, Is a Platform for Cdk1 to Phosphorylate Epsin during the Switch Off of Endocytosis in Mitosis J. Biol. Chem., August 15, 2003; 278(33): 30597 - 30604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. K. Hussain, M. Yamabhai, A. L. Bhakar, M. Metzler, S. S. G. Ferguson, M. R. Hayden, P. S. McPherson, and B. K. Kay A Role for Epsin N-terminal Homology/AP180 N-terminal Homology (ENTH/ANTH) Domains in Tubulin Binding J. Biol. Chem., August 1, 2003; 278(31): 28823 - 28830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Aguilar, H. A. Watson, and B. Wendland The Yeast Epsin Ent1 Is Recruited to Membranes through Multiple Independent Interactions J. Biol. Chem., March 14, 2003; 278(12): 10737 - 10743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Conner and S. L. Schmid Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis J. Cell Biol., March 4, 2002; 156(5): 921 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Santini, I. Gaidarov, and J. H. Keen G protein-coupled receptor/arrestin3 modulation of the endocytic machinery J. Cell Biol., February 18, 2002; 156(4): 665 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Watson, M. J. T. V. Cope, A. C. Groen, D. G. Drubin, and B. Wendland In Vivo Role for Actin-regulating Kinases in Endocytosis and Yeast Epsin Phosphorylation Mol. Biol. Cell, November 1, 2001; 12(11): 3668 - 3679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Okamoto, J. McKinney, and Y. Y. Jeng Clathrin in mitotic spindles Am J Physiol Cell Physiol, August 1, 2000; 279(2): C369 - C374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Faundez and R. B. Kelly The AP-3 Complex Required for Endosomal Synaptic Vesicle Biogenesis is Associated with a Casein Kinase Ialpha -Like Isoform Mol. Biol. Cell, August 1, 2000; 11(8): 2591 - 2604. [Abstract] [Full Text]
|
|
|
|
|
|
L. Gerez, K. Mohrmann, M. van Raak, M. Jongeneelen, X. Z. Zhou, K. P. Lu, and P. van der Sluijs Accumulation of rab4GTP in the Cytoplasm and Association with the Peptidyl-Prolyl Isomerase Pin1 during Mitosis Mol. Biol. Cell, July 1, 2000; 11(7): 2201 - 2211. [Abstract] [Full Text]
|
|
|
|
|
|
C. Daly, M. Sugimori, J. E. Moreira, E. B. Ziff, and R. Llinas Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles PNAS, May 23, 2000; 97(11): 6120 - 6125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hyman, H. Chen, P. P. Di Fiore, P. De Camilli, and A. T. Brunger Epsin 1 Undergoes Nucleocytosolic Shuttling and its Eps15 Interactor NH2-terminal Homology (ENTH) Domain, Structurally Similar to Armadillo and HEAT Repeats, Interacts with the Transcription Factor Promyelocytic Leukemia Zn2+ Finger Protein (PLZF) J. Cell Biol., May 1, 2000; 149(3): 537 - 546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Powell, V. A. Valova, C. S. Malladi, O. N. Jensen, M. R. Larsen, and P. J. Robinson Phosphorylation of Dynamin I on Ser-795 by Protein Kinase C Blocks Its Association with Phospholipids J. Biol. Chem., April 14, 2000; 275(16): 11610 - 11617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Rosenthal, H. Chen, V. I. Slepnev, L. Pellegrini, A. E. Salcini, P. P. Di Fiore, and P. De Camilli The Epsins Define a Family of Proteins That Interact with Components of the Clathrin Coat and Contain a New Protein Module J. Biol. Chem., November 26, 1999; 274(48): 33959 - 33965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Traub, M. A. Downs, J. L. Westrich, and D. H. Fremont Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly PNAS, August 3, 1999; 96(16): 8907 - 8912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kariya, S. Koyama, S. Nakashima, T. Oshiro, K. Morinaka, and A. Kikuchi Regulation of Complex Formation of POB1/Epsin/Adaptor Protein Complex 2 by Mitotic Phosphorylation J. Biol. Chem., June 9, 2000; 275(24): 18399 - 18406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Floyd, E. B. Porro, V. I. Slepnev, G.-C. Ochoa, L.-H. Tsai, and P. De Camilli Amphiphysin 1 Binds the Cyclin-dependent Kinase (cdk) 5 Regulatory Subunit p35 and Is Phosphorylated by cdk5 and cdc2 J. Biol. Chem., March 9, 2001; 276(11): 8104 - 8110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-W. Cho, M.-H. Kim, Y.-G. Chai, M. L. Gilmor, A. I. Levey, and L. B. Hersh Phosphorylation of the Rat Vesicular Acetylcholine Transporter J. Biol. Chem., June 23, 2000; 275(26): 19942 - 19948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K.A. deHart, J. D. Schnell, D. A. Allen, and L. Hicke The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast J. Cell Biol., January 21, 2002; 156(2): 241 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Conner and S. L. Schmid Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis J. Cell Biol., March 4, 2002; 156(5): 921 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Santini, I. Gaidarov, and J. H. Keen G protein-coupled receptor/arrestin3 modulation of the endocytic machinery J. Cell Biol., February 18, 2002; 156(4): 665 - 676. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
