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J Biol Chem, Vol. 274, Issue 6, 3573-3579, February 5, 1999
From the Centre de Recherche de Biochimie Macromoléculaire,
CNRS UPR 1086, 1919 Route de Mende, 34293 Montpellier cedex 5, France
and § IFREMER, 17390 La Tremblade, France
X-PAKs are involved in negative control of the
process of oocyte maturation in Xenopus (). In the present
study, we define more precisely the events targetted by the kinase in
the inhibition of the G2/M transition. We show that
microinjection of recombinant X-PAK1-Cter active kinase into
progesterone-treated oocytes prevents c-Mos accumulation and activation
of both MAPK and maturation-promoting factor (MPF). In conditions
permissive for MAPK activation, MPF activation still fails. We
demonstrate that a constitutive truncated version of X-PAK1
(X-PAK1-Cter) does not prevent the association of cyclin B with
p34cdc2 but rather prevents the activation of the inactive
complexes present in the oocyte. Proteins participating in the MPF
amplification loop, including the Cdc25-activating Polo-like kinase are
all blocked. Indeed, using active MPF, the amplification loop is not turned on in the presence of X-PAK1. Our results indicate that X-PAK
and protein kinase A targets in the control of oocyte maturation are
similar and furthermore that this negative regulation is not restricted
to meiosis, because we demonstrate that G2/M progression is
also prevented in Xenopus cycling extracts in the presence of active X-PAK1.
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