| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 6, 3859-3864, February 5, 1999
,¶
From the Pokeweed antiviral protein (PAP), a 29-kDa
ribosome-inactivating protein, catalytically removes an adenine residue
from the conserved Biotechnology Center for Agriculture and the
Environment and Department of Plant Pathology, Rutgers University, New
Brunswick, New Jersey 08901-8520 and § Department of
Molecular Genetics and Microbiology and The Cancer Institute of New
Jersey and ¶ Graduate Program in Molecular Biosciences at Rutgers,
University of Medicine and Dentistry of New Jersey,
Piscataway, New Jersey 08854
-sarcin loop of the large rRNA, thereby
preventing the binding of eEF-2·GTP complex during protein
elongation. Because the -sarcin loop has been placed near the
peptidyltransferase center in Escherichia coli ribosomes,
we investigated the effects of alterations at the peptidyltransferase
center on the activity of PAP. We demonstrate here that a chromosomal
mutant of yeast, harboring the mak8-1 allele of
peptidyltransferase-linked ribosomal protein L3 (RPL3), is
resistant to the cytostatic effects of PAP. Unlike wild-type yeast,
ribosomes from mak8-1 cells are not depurinated when PAP
expression is induced in vivo, indicating that wild-type L3
is required for ribosome depurination. Co-immunoprecipitation studies
show that PAP binds directly to L3 or Mak8-1p in vitro but
does not physically interact with ribosome-associated Mak8-1p. L3 is
required for PAP to bind to ribosomes and depurinate the 25 S rRNA,
suggesting that it is located in close proximity to the -sarcin
loop. These results demonstrate for the first time that a ribosomal
protein provides a receptor site for an ribosome-inactivating protein
and allows depurination of the target adenine.
This article has been cited by other articles:
|
|
 |
|
  A. N.-S. Mak, Y.-T. Wong, Y.-J. An, S.-S. Cha, K.-H. Sze, S. W.-N. Au, K.-B. Wong, and P.-C. Shaw Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site Nucleic Acids Res., September 25, 2007; 35(18): 6259 - 6267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S.B. Chan, L.-O. Chu, K.-M. Lee, P. H.M. Too, K.-W. Ma, K.-H. Sze, G. Zhu, P.-C. Shaw, and K.-B. Wong Interaction between trichosanthin, a ribosome-inactivating protein, and the ribosomal stalk protein P2 by chemical shift perturbation and mutagenesis analyses Nucleic Acids Res., March 12, 2007; 35(5): 1660 - 1672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mansouri, E. Nourollahzadeh, and K. A. Hudak Pokeweed antiviral protein depurinates the sarcin/ricin loop of the rRNA prior to binding of aminoacyl-tRNA to the ribosomal A-site RNA, September 1, 2006; 12(9): 1683 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wang and K. A. Hudak A novel interaction of pokeweed antiviral protein with translation initiation factors 4G and iso4G: a potential indirect mechanism to access viral RNAs Nucleic Acids Res., February 21, 2006; 34(4): 1174 - 1181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Hudak, B. A. Parikh, R. Di, M. Baricevic, M. Santana, M. Seskar, and N. E. Tumer Generation of pokeweed antiviral protein mutations in Saccharomyces cerevisiae: evidence that ribosome depurination is not sufficient for cytotoxicity Nucleic Acids Res., August 10, 2004; 32(14): 4244 - 4256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-H. Yang, M. Wieczorck, M. C. Allen, and T. M. Nett Cytotoxic Activity of Gonadotropin-Releasing Hormone (GnRH)-Pokeweed Antiviral Protein Conjugates in Cell Lines Expressing GnRH Receptors Endocrinology, April 1, 2003; 144(4): 1456 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Parikh, C. Coetzer, and N. E. Tumer Pokeweed Antiviral Protein Regulates the Stability of Its Own mRNA by a Mechanism That Requires Depurination but Can Be Separated from Depurination of the alpha -Sarcin/Ricin Loop of rRNA J. Biol. Chem., October 25, 2002; 277(44): 41428 - 41437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. PEUMANS, Q. HAO, and E. J. M. VAN DAMME Ribosome-inactivating proteins from plants: more than RNA N-glycosidases? FASEB J, July 1, 2001; 15(9): 1493 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. M. Uckun, K. Bellomy, K. O'Neill, Y. Messinger, T. Johnson, and C.-L. Chen Toxicity, Biological Activity, and Pharmacokinetics of TXU (Anti-CD7)-Pokeweed Antiviral Protein in Chimpanzees and Adult Patients Infected with Human Immunodeficiency Virus J. Pharmacol. Exp. Ther., December 1, 1999; 291(3): 1301 - 1307. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
