| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 6, 3887-3896, February 5, 1999
From the Cell Biology Section, Laboratory of Pulmonary
Pathobiology, NIEHS, National Institutes of Health,
Research Triangle Park, North Carolina 27709
The transglutaminase I (TGase I) gene encodes an
enzyme that catalyzes the cross-linking of structural proteins involved
in the formation of the cornified envelope during squamous cell
differentiation. To identify DNA elements important for the
transcriptional control of the TGase I gene, we analyzed the ability of
a 2.9-kilobase pair (kb) upstream regulatory region to control the
expression of a reporter gene in vivo and in
vitro. Transgenic mice bearing the pTG(
Regulation of the Transglutaminase I Gene
IDENTIFICATION OF DNA ELEMENTS INVOLVED IN ITS TRANSCRIPTIONAL
CONTROL IN TRACHEOBRONCHIAL EPITHELIAL CELLS
2.9kb)CAT construct
exhibited the same pattern of tissue-specific expression of CAT as
reported for TGase I. Deletion analysis in transiently transfected
rabbit tracheal epithelial cells indicated that two sequences from bp
490 to 470 and from 54 to 37 are involved in the activation of
TGase I transcription. Point mutation analysis and mobility shift
assays showed that the sequence located between 54 and 37 is a
functional Sp1-like transcription element. Sp1 and Sp3, but not Sp2,
are part of nuclear protein complexes from differentiated RbTE cells
binding to this site. The element TGATGTCA between bp 490 and 470
is contained in a larger 22-bp palindrome and resembles the consensus
cAMP response element-binding protein (CREB)/AP-1 element recognized by
dimeric complexes of members of the CREB, ATF, Fos, and Jun families.
Mutations in this sequence greatly reduced promoter activity.
Supershift analysis identified CREB1, JunB, c-Fos, Fra-1, and c-Jun in
protein complexes isolated from differentiated rabbit tracheal
epithelial cells binding to this site. Our study shows that the Sp1-
and CREB/AP-1-like sites act in concert to stimulate transcription of
the TGase I gene. The 2.9-kb promoter region could guide expression of
specific genes in the granular layer of the epidermis and could be
useful in gene therapy.
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X. W. Wang, N. S. Tan, B. Ho, and J. L. Ding Evidence for the ancient origin of the NF-{kappa}B/I{kappa}B cascade: Its archaic role in pathogen infection and immunity. PNAS, March 14, 2006; 103(11): 4204 - 4209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rajakumar, S. Thamotharan, N. Raychaudhuri, R. K. Menon, and S. U. Devaskar Trans-activators Regulating Neuronal Glucose Transporter Isoform-3 Gene Expression in Mammalian Neurons J. Biol. Chem., June 18, 2004; 279(25): 26768 - 26779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Martinet, L. Bonnard, V. Regnault, E. Picard, L. Burke, J. Siat, G. Grosdidier, Y. Martinet, and J.-M. Vignaud In Vivo Transglutaminase Type 1 Expression in Normal Lung, Preinvasive Bronchial Lesions, and Lung Cancer Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 428 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. M. Reddy and B. T. Mossman Role and regulation of activator protein-1 in toxicant-induced responses of the lung Am J Physiol Lung Cell Mol Physiol, December 1, 2002; 283(6): L1161 - L1178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Serewko, C. Popa, A. L. Dahler, L. Smith, G. M. Strutton, W. Coman, A. J. Dicker, and N. A. Saunders Alterations in Gene Expression and Activity during Squamous Cell Carcinoma Development Cancer Res., July 1, 2002; 62(13): 3759 - 3765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Rutberg, T. L. Adams, A. Glick, M. T. Bonovich, C. Vinson, and S. H. Yuspa Activator Protein 1 Transcription Factors Are Fundamental to v-rasHa-induced Changes in Gene Expression in Neoplastic Keratinocytes Cancer Res., November 1, 2000; 60(22): 6332 - 6338. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Y. Kim, H. Adachi, J. S. Koo, and A. M. Jetten Induction of the Cytochrome P450 Gene CYP26 during Mucous Cell Differentiation of Normal Human Tracheobronchial Epithelial Cells Mol. Pharmacol., September 1, 2000; 58(3): 483 - 490. [Abstract] [Full Text]
|
|
|
|
|
|
S. Kurebayashi, E. Ueda, M. Sakaue, D. D. Patel, A. Medvedev, F. Zhang, and A. M. Jetten Retinoid-related orphan receptor gamma (RORgamma ) is essential for lymphoid organogenesis and controls apoptosis during thymopoiesis PNAS, August 29, 2000; 97(18): 10132 - 10137. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
