HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kose, S. Articles by Yoneda, Y. Search for Related Content PubMed PubMed Citation Articles by Kose, S. Articles by Yoneda, Y.

J Biol Chem, Vol. 274, Issue 7, 3946-3952, February 12, 1999

-Subunit of Nuclear Pore-targeting Complex (Importin-) Can Be Exported from the Nucleus in a Ran-independent Manner

Shingo Kose, Naoko Imamoto, Taro Tachibana, Minoru Yoshida^§, and Yoshihiro Yoneda

From the  Department of Anatomy and Cell Biology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan and the ^§ Department of Biotechnology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan

The nuclear export of importin- is mediated by CAS, which is related to importin-, whereas the mechanism for the export of importin- remains unclear. In this study, we demonstrate that the nuclear export of importin- is mediated by the nuclear pore complex-binding domain of this molecule. Insensitivity to leptomycin B indicates that its export is not mediated by a leucine-rich nuclear export signal-specific receptor, CRM1. Furthermore, the nuclear export of importin- was not inhibited by co-injection with a GTPase-deficient Ran mutant (G19V). The cell line tsBN2 contains a temperature-sensitive point mutation in the RCC1 gene, which encodes a guanine nucleotide exchange factor of Ran. At the nonpermissive temperature, importin- was exported from the nucleus of these cells, even when RanGAP1, a GTPase-activating protein for Ran, was co-injected. These results not only provide support for the view that Ran-dependent GTP hydrolysis is not required for the nuclear export of importin- but also indicate that nuclear RanGTP is not essential for its export. As a result, we propose that importin- can be recycled from the nucleus alone in a Ran-independent manner.

---

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.

This article has been cited by other articles:

				R. B. Kopito and M. Elbaum Reversibility in nucleocytoplasmic transport PNAS, July 31, 2007; 104(31): 12743 - 12748. [Abstract] [Full Text] [PDF]

				A. Kurisaki, K. Kurisaki, M. Kowanetz, H. Sugino, Y. Yoneda, C.-H. Heldin, and A. Moustakas The Mechanism of Nuclear Export of Smad3 Involves Exportin 4 and Ran Mol. Cell. Biol., February 15, 2006; 26(4): 1318 - 1332. [Abstract] [Full Text] [PDF]

				M. Koike, S. Kose, M. Furuta, N. Taniguchi, F. Yokoya, Y. Yoneda, and N. Imamoto {beta}-Catenin Shows an Overlapping Sequence Requirement but Distinct Molecular Interactions for Its Bidirectional Passage through Nuclear Pores J. Biol. Chem., August 6, 2004; 279(32): 34038 - 34047. [Abstract] [Full Text] [PDF]

				X. Zhang, M. Yamada, N. Mabuchi, and H. Shida Cellular Requirements for CRM1 Import and Export J. Biochem., November 1, 2003; 134(5): 759 - 764. [Abstract] [Full Text] [PDF]

				Y. Eura, N. Ishihara, S. Yokota, and K. Mihara Two Mitofusin Proteins, Mammalian Homologues of FZO, with Distinct Functions Are Both Required for Mitochondrial Fusion J. Biochem., September 1, 2003; 134(3): 333 - 344. [Abstract] [Full Text] [PDF]

				J. Bednenko, G. Cingolani, and L. Gerace Importin {beta} contains a COOH-terminal nucleoporin binding region important for nuclear transport J. Cell Biol., August 4, 2003; 162(3): 391 - 401. [Abstract] [Full Text] [PDF]

				J. Katahira, K. Straesser, T. Saiwaki, Y. Yoneda, and E. Hurt Complex Formation between Tap and p15 Affects Binding to FG-repeat Nucleoporins and Nucleocytoplasmic Shuttling J. Biol. Chem., March 8, 2002; 277(11): 9242 - 9246. [Abstract] [Full Text] [PDF]

				S. M. Steggerda, B. E. Black, and B. M. Paschal Monoclonal Antibodies to NTF2 Inhibit Nuclear Protein Import by Preventing Nuclear Translocation of the GTPase Ran Mol. Biol. Cell, February 1, 2000; 11(2): 703 - 719. [Abstract] [Full Text]

				J. Hood, J. Casolari, and P. Silver Nup2p is located on the nuclear side of the nuclear pore complex and coordinates Srp1p/importin-alpha export J. Cell Sci., January 4, 2000; 113(8): 1471 - 1480. [Abstract] [PDF]

				J. Liu and D. B. DeFranco Protracted Nuclear Export of Glucocorticoid Receptor Limits Its Turnover and Does Not Require the Exportin 1/CRM1-Directed Nuclear Export Pathway Mol. Endocrinol., January 1, 2000; 14(1): 40 - 51. [Abstract] [Full Text]

				E. Nagoshi, N. Imamoto, R. Sato, and Y. Yoneda Nuclear Import of Sterol Regulatory Element-binding Protein-2, a Basic Helix-Loop-Helix-Leucine Zipper (bHLH-Zip)-containing Transcription Factor, Occurs through the Direct Interaction of Importin beta with HLH-Zip Mol. Biol. Cell, July 1, 1999; 10(7): 2221 - 2233. [Abstract] [Full Text]

				A. Eleftheriou, M. Yoshida, and B. R. Henderson Nuclear Export of Human beta -Catenin Can Occur Independent of CRM1 and the Adenomatous Polyposis Coli Tumor Suppressor J. Biol. Chem., July 6, 2001; 276(28): 25883 - 25888. [Abstract] [Full Text] [PDF]

				E. R. Griffis, N. Altan, J. Lippincott-Schwartz, and M. A. Powers Nup98 Is a Mobile Nucleoporin with Transcription-dependent Dynamics Mol. Biol. Cell, April 1, 2002; 13(4): 1282 - 1297. [Abstract] [Full Text] [PDF]