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J Biol Chem, Vol. 274, Issue 7, 4155-4159, February 12, 1999
From the Department of Cell and Structural Biology, University of
Illinois, Urbana-Champaign, Urbana, Illinois 61801
Eukaryotic chromosomal origins of replication are
best defined in Saccharomyces cerevisiae. Previous analysis
of yeast origins suggests that they are relatively simple structures
comprised of three or four small DNA sequence elements contained within approximately 100-200-base pair regions (Gilbert, D. M. (1998) Curr. Opin. Genet. Dev. 8, 194-199). In contrast, the
sequence elements that may comprise origins in multicellular eukaryotes are largely unknown. The yeast HMR-E region is both a
chromosomal origin of replication and a silencer that represses
transcription of adjacent genes through a position effect. The analysis
presented here indicated that HMR-E had a novel DNA
structure that was more complex than defined for other yeast origins,
and thus revealed that there is variation in the structural complexity
of yeast origins. In contrast to "simple" yeast origins, the origin
at HMR-E consisted of at least three independent subregions
that had the capacity to initiate replication. We have termed
HMR-E a compound origin to reflect its structural
complexity. Furthermore, only one origin within the compound origin was
a silencer.
Identification of a Compound Origin of Replication at the
HMR-E Locus in Saccharomyces
cerevisiae
Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
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