HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Attaphitaya, S. Articles by Melvin, J. E. Search for Related Content PubMed PubMed Citation Articles by Attaphitaya, S. Articles by Melvin, J. E. Social Bookmarking                      What's this?

J Biol Chem, Vol. 274, Issue 7, 4383-4388, February 12, 1999

Molecular Cloning and Functional Expression of a Rat Na⁺/H⁺ Exchanger (NHE5) Highly Expressed in Brain

Surat Attaphitaya^§, Keerang Park, and James E. Melvin

From the  Center for Oral Biology and the ^§ Department of Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

We report here the cloning, primary structure, heterologous expression, tissue distribution, and localization of a cDNA encoding rat NHE5, a fifth member of the mammalian plasma membrane Na⁺/H⁺ exchanger (NHE) gene family. The full-length open reading frame as well as 34 nucleotides of 5'-untranslated and 1443 nucleotides of 3'-untranslated sequences were obtained using a polymerase chain reaction strategy involving reverse transcription-polymerase chain reaction and 5'/3'-rapid amplification of cDNA ends. The NHE5 cDNA encodes a protein of 898 amino acids with a calculated M_r of 99,044 and is predicted to contain 11-13 transmembrane domains. An amino acid comparison of the coding region of rat NHE5 reveals 95% identity with human NHE5. Northern hybridization analysis showed that high level expression of NHE5 mRNA is restricted to brain. Transfection of the coding region of rat NHE5 into NHE-deficient PS120 cells resulted in Na⁺/H⁺ exchange activity that was relatively insensitive to the amiloride analogue, 5-(N-ethyl-N-isopropyl) amiloride, with a half-maximal inhibitory concentration (IC₅₀) of 1.53 ± 0.25 µM. In situ hybridization of rat brain sections revealed significant NHE5 mRNA levels in the dentate gyrus with lower levels observed in the hippocampus and cerebral cortex. These results suggest a specialized role for this fifth NHE isoform in neuronal tissues.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				G. H. Diering, J. Church, and M. Numata Secretory Carrier Membrane Protein 2 Regulates Cell-surface Targeting of Brain-enriched Na+/H+ Exchanger NHE5 J. Biol. Chem., May 15, 2009; 284(20): 13892 - 13903. [Abstract] [Full Text] [PDF]

				H. Xu, H. Chen, J. Dong, J. Li, R. Chen, J. K. Uno, and F. K. Ghishan Tumor necrosis factor-{alpha} downregulates intestinal NHE8 expression by reducing basal promoter activity Am J Physiol Cell Physiol, March 1, 2009; 296(3): C489 - C497. [Abstract] [Full Text] [PDF]

				R. Ohgaki, N. Fukura, M. Matsushita, K. Mitsui, and H. Kanazawa Cell Surface Levels of Organellar Na+/H+ Exchanger Isoform 6 Are Regulated by Interaction with RACK1 J. Biol. Chem., February 15, 2008; 283(7): 4417 - 4429. [Abstract] [Full Text] [PDF]

				M. Donowitz and X. Li Regulatory Binding Partners and Complexes of NHE3 Physiol Rev, July 1, 2007; 87(3): 825 - 872. [Abstract] [Full Text] [PDF]

				M. Matsushita, Y. Sano, S. Yokoyama, T. Takai, H. Inoue, K. Mitsui, K. Todo, H. Ohmori, and H. Kanazawa Loss of calcineurin homologous protein-1 in chicken B lymphoma DT40 cells destabilizes Na+/H+ exchanger isoform-1 protein Am J Physiol Cell Physiol, July 1, 2007; 293(1): C246 - C254. [Abstract] [Full Text] [PDF]

				L. A. Shimoda, M. Fallon, S. Pisarcik, J. Wang, and G. L. Semenza HIF-1 regulates hypoxic induction of NHE1 expression and alkalinization of intracellular pH in pulmonary arterial myocytes Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L941 - L949. [Abstract] [Full Text] [PDF]

				A. K. Pullikuth, K. Aimanova, W. Kang'ethe, H. R. Sanders, and S. S. Gill Molecular characterization of sodium/proton exchanger 3 (NHE3) from the yellow fever vector, Aedes aegypti J. Exp. Biol., September 15, 2006; 209(18): 3529 - 3544. [Abstract] [Full Text] [PDF]

				E. J. Rios, M. Fallon, J. Wang, and L. A. Shimoda Chronic hypoxia elevates intracellular pH and activates Na+/H+ exchange in pulmonary arterial smooth muscle cells Am J Physiol Lung Cell Mol Physiol, November 1, 2005; 289(5): L867 - L874. [Abstract] [Full Text] [PDF]

				H. Xu, R. Chen, and F. K. Ghishan Subcloning, localization, and expression of the rat intestinal sodium-hydrogen exchanger isoform 8 Am J Physiol Gastrointest Liver Physiol, July 1, 2005; 289(1): G36 - G41. [Abstract] [Full Text] [PDF]

				C. L. Brett, M. Donowitz, and R. Rao Evolutionary origins of eukaryotic sodium/proton exchangers Am J Physiol Cell Physiol, February 1, 2005; 288(2): C223 - C239. [Abstract] [Full Text] [PDF]

				N. Nakamura, S. Tanaka, Y. Teko, K. Mitsui, and H. Kanazawa Four Na+/H+ Exchanger Isoforms Are Distributed to Golgi and Post-Golgi Compartments and Are Involved in Organelle pH Regulation J. Biol. Chem., January 14, 2005; 280(2): 1561 - 1572. [Abstract] [Full Text] [PDF]

				C. Sheldon, A. Diarra, Y. M. Cheng, and J. Church Sodium Influx Pathways during and after Anoxia in Rat Hippocampal Neurons J. Neurosci., December 8, 2004; 24(49): 11057 - 11069. [Abstract] [Full Text] [PDF]

				D. A. Brown, J. E. Melvin, and D. I. Yule Critical role for NHE1 in intracellular pH regulation in pancreatic acinar cells Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G804 - G812. [Abstract] [Full Text] [PDF]

				Y. Wang, J. W. Meyer, M. Ashraf, and G. E. Shull Mice With a Null Mutation in the NHE1 Na+-H+ Exchanger Are Resistant to Cardiac Ischemia-Reperfusion Injury Circ. Res., October 17, 2003; 93(8): 776 - 782. [Abstract] [Full Text] [PDF]

				M. CHESLER Regulation and Modulation of pH in the Brain Physiol Rev, October 1, 2003; 83(4): 1183 - 1221. [Abstract] [Full Text] [PDF]

				K. Szaszi, A. Paulsen, E. Z. Szabo, M. Numata, S. Grinstein, and J. Orlowski Clathrin-mediated Endocytosis and Recycling of the Neuron-specific Na+/H+ Exchanger NHE5 Isoform. REGULATION BY PHOSPHATIDYLINOSITOL 3'-KINASE AND THE ACTIN CYTOSKELETON J. Biol. Chem., November 1, 2002; 277(45): 42623 - 42632. [Abstract] [Full Text] [PDF]

				K. Nehrke and J. E. Melvin The NHX Family of Na+-H+ Exchangers in Caenorhabditis elegans J. Biol. Chem., August 2, 2002; 277(32): 29036 - 29044. [Abstract] [Full Text] [PDF]

				C. V. Rieder and L. Fliegel Developmental regulation of Na+/H+ exchanger expression in fetal and neonatal mice Am J Physiol Heart Circ Physiol, July 1, 2002; 283(1): H273 - H283. [Abstract] [Full Text] [PDF]

				S. Attaphitaya, K. Nehrke, and J. E. Melvin Acute inhibition of brain-specific Na+/H+ exchanger isoform 5 by protein kinases A and C and cell shrinkage Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1146 - C1157. [Abstract] [Full Text] [PDF]

				H. Yao, X.-Q. Gu, R. M. Douglas, and G. G. Haddad Role of Na+/H+ exchanger during O2 deprivation in mouse CA1 neurons Am J Physiol Cell Physiol, October 1, 2001; 281(4): C1205 - C1210. [Abstract] [Full Text] [PDF]

				R. Chambrey, P. L. St. John, D. Eladari, F. Quentin, D. G. Warnock, D. R. Abrahamson, R.-A. Podevin, and M. Paillard Localization and functional characterization of Na+/H+ exchanger isoform NHE4 in rat thick ascending limbs Am J Physiol Renal Physiol, October 1, 2001; 281(4): F707 - F717. [Abstract] [Full Text] [PDF]

				C. Bagnis, M. Marsolais, D. Biemesderfer, R. Laprade, and S. Breton Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis Am J Physiol Renal Physiol, March 1, 2001; 280(3): F426 - F436. [Abstract] [Full Text] [PDF]

				M. E. Giannakou and J. A. T. Dow Characterization of the Drosophila melanogaster alkali-metal/proton exchanger (NHE) gene family J. Exp. Biol., January 11, 2001; 204(21): 3703 - 3716. [Abstract] [Full Text] [PDF]

				P. Bijlenga, J.-H. Liu, E. Espinos, C.-A. Haenggeli, J. Fischer-Lougheed, C. R. Bader, and L. Bernheim T-type alpha 1H Ca2+ channels are involved in Ca2+ signaling during terminal differentiation (fusion) of human myoblasts PNAS, June 20, 2000; 97(13): 7627 - 7632. [Abstract] [Full Text] [PDF]

				E. Z. Szabo, M. Numata, G. E. Shull, and J. Orlowski Kinetic and Pharmacological Properties of Human Brain Na+/H+ Exchanger Isoform 5 Stably Expressed in Chinese Hamster Ovary Cells J. Biol. Chem., February 25, 2000; 275(9): 6302 - 6307. [Abstract] [Full Text] [PDF]

				H.-V. Nguyen, G. E Shull, and J. E Melvin Muscarinic receptor-induced acidification in sublingual mucous acinar cells: loss of pH recovery in Na+-H+ exchanger-1 deficient mice J. Physiol., February 15, 2000; 523(1): 139 - 146. [Abstract] [Full Text] [PDF]

				R. L. Evans, S. M. Bell, P. J. Schultheis, G. E. Shull, and J. E. Melvin Targeted Disruption of the Nhe1 Gene Prevents Muscarinic Agonist-induced Up-regulation of Na+/H+ Exchange in Mouse Parotid Acinar Cells J. Biol. Chem., October 8, 1999; 274(41): 29025 - 29030. [Abstract] [Full Text] [PDF]

				N. R. Baird, J. Orlowski, E. Z. Szabo, H. C. Zaun, P. J. Schultheis, A. G. Menon, and G. E. Shull Molecular Cloning, Genomic Organization, and Functional Expression of Na+/H+ Exchanger Isoform 5 (NHE5) from Human Brain J. Biol. Chem., February 12, 1999; 274(7): 4377 - 4382. [Abstract] [Full Text] [PDF]