| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 274, Issue 8, 4620-4625, February 19, 1999
From the Department of Pharmacology, Yale University School of
Medicine, New Haven, Connecticut 06520
A protein fraction of mitochondria from human
acute lymphocytic leukemia cells, which could be reconstituted into
proteoliposomes to have dCTP transport activity, has been partially
purified by hydroxyapatite and blue Sepharose chromatography. The dCTP
transport activity in proteoliposomes was time-dependent
and could be activated by Ca2+ and to a lesser extent
by Mg2+. None of the other divalent cations tested could
activate the transport activity. The Km value of
dCTP in the presence of Ca2+ was shown to be 3 µM. dCDP but not dCMP or dCyd could inhibit the transport
activity. Other deoxynucleoside triphosphates could also inhibit the
uptake of dCTP with the potency dGTP = dATP > TTP. Although
ATP could competitively inhibit dCTP uptake with a
Ki value of 8 µM, the reconstituted
dCTP uptake activity was not sensitive to the ATP/ADP carrier inhibitor
atractyloside or the sulfhydryl reagent N-ethylmaleimide.
This suggests that the dCTP transport system studied is not the same as
the ATP/ADP carrier. In conclusion, these studies describe the first
functionally reconstituted mitochondrial carrier that displays an
efficient transport activity for dCTP.
This article has been cited by other articles:
|
|
 |
|
  A. Basavapathruni and K. S. Anderson Reverse transcription of the HIV-1 pandemic FASEB J, December 1, 2007; 21(14): 3795 - 3808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Solaroli, X. Zheng, M. Johansson, J. Balzarini, and A. Karlsson Mitochondrial Expression of the Drosophila melanogaster Multisubstrate Deoxyribonucleoside Kinase Mol. Pharmacol., December 1, 2007; 72(6): 1593 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rampazzo, S. Fabris, E. Franzolin, K. Crovatto, M. Frangini, and V. Bianchi Mitochondrial Thymidine Kinase and the Enzymatic Network Regulating Thymidine Triphosphate Pools in Cultured Human Cells J. Biol. Chem., November 30, 2007; 282(48): 34758 - 34769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Mathews and S. Song Maintaining precursor pools for mitochondrial DNA replication FASEB J, August 1, 2007; 21(10): 2294 - 2303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Pontarin, P. Ferraro, P. Hakansson, L. Thelander, P. Reichard, and V. Bianchi p53R2-dependent Ribonucleotide Reduction Provides Deoxyribonucleotides in Quiescent Human Fibroblasts in the Absence of Induced DNA Damage J. Biol. Chem., June 8, 2007; 282(23): 16820 - 16828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Satrustegui, B. Pardo, and A. del Arco Mitochondrial Transporters as Novel Targets for Intracellular Calcium Signaling Physiol Rev, January 1, 2007; 87(1): 29 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Mathews DNA precursor metabolism and genomic stability FASEB J, July 1, 2006; 20(9): 1300 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Lam, C. Chen, S. Ruan, C.-H. Leung, and Y.-C. Cheng Expression of Deoxynucleotide Carrier Is Not Associated with the Mitochondrial DNA Depletion Caused by Anti-HIV Dideoxynucleoside Analogs and Mitochondrial dNTP Uptake Mol. Pharmacol., February 1, 2005; 67(2): 408 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Lai, C.-M. Tse, and J. D. Unadkat Mitochondrial Expression of the Human Equilibrative Nucleoside Transporter 1 (hENT1) Results in Enhanced Mitochondrial Toxicity of Antiviral Drugs J. Biol. Chem., February 6, 2004; 279(6): 4490 - 4497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Song, L. J. Wheeler, and C. K. Mathews Deoxyribonucleotide Pool Imbalance Stimulates Deletions in HeLa Cell Mitochondrial DNA J. Biol. Chem., November 7, 2003; 278(45): 43893 - 43896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pontarin, L. Gallinaro, P. Ferraro, P. Reichard, and V. Bianchi Origins of mitochondrial thymidine triphosphate: Dynamic relations to cytosolic pools PNAS, October 14, 2003; 100(21): 12159 - 12164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-W. Taanman, J. R. Muddle, and A. C. Muntau Mitochondrial DNA depletion can be prevented by dGMP and dAMP supplementation in a resting culture of deoxyguanosine kinase-deficient fibroblasts Hum. Mol. Genet., August 1, 2003; 12(15): 1839 - 1845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Lewis Defective mitochondrial DNA replication and NRTIs: pathophysiological implications in AIDS cardiomyopathy Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H1 - H9. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mancuso, L. Salviati, S. Sacconi, D. Otaegui, P. Camano, A. Marina, S. Bacman, C.T. Moraes, J.R. Carlo, M. Garcia, et al. Mitochondrial DNA depletion: Mutations in thymidine kinase gene with myopathy and SMA Neurology, October 22, 2002; 59(8): 1197 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Liou, G. E. Dutschman, W. Lam, Z. Jiang, and Y.-C. Cheng Characterization of Human UMP/CMP Kinase and Its Phosphorylation of D- and L-Form Deoxycytidine Analogue Monophosphates Cancer Res., March 1, 2002; 62(6): 1624 - 1631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J White Mitochondrial toxicity and HIV therapy Sex. Transm. Inf., June 1, 2001; 77(3): 158 - 173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Sjöberg, L. Wang, and S. Eriksson Antiviral Guanosine Analogs as Substrates for Deoxyguanosine Kinase: Implications for Chemotherapy Antimicrob. Agents Chemother., March 1, 2001; 45(3): 739 - 742. [Abstract] [Full Text]
|
|
|
|
|
|
C. Rampazzo, L. Gallinaro, E. Milanesi, E. Frigimelica, P. Reichard, and V. Bianchi A deoxyribonucleotidase in mitochondria: Involvement in regulation of dNTP pools and possible link to genetic disease PNAS, July 18, 2000; 97(15): 8239 - 8244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Van Rompay, M. Johansson, and A. Karlsson Phosphorylation of Deoxycytidine Analog Monophosphates by UMP-CMP Kinase: Molecular Characterization of the Human Enzyme Mol. Pharmacol., September 1, 1999; 56(3): 562 - 569. [Abstract] [Full Text]
|
|
|
|
|
|
T. G. Petrakis, E. Ktistaki, L. Wang, S. Eriksson, and I. Talianidis Cloning and Characterization of Mouse Deoxyguanosine Kinase. EVIDENCE FOR A CYTOPLASMIC ISOFORM J. Biol. Chem., August 27, 1999; 274(35): 24726 - 24730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhu, M. Johansson, and A. Karlsson Incorporation of Nucleoside Analogs into Nuclear or Mitochondrial DNA Is Determined by the Intracellular Phosphorylation Site J. Biol. Chem., August 25, 2000; 275(35): 26727 - 26731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Lim and W. C. Copeland Differential Incorporation and Removal of Antiviral Deoxynucleotides by Human DNA Polymerase gamma J. Biol. Chem., June 22, 2001; 276(26): 23616 - 23623. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
