JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyagi, T.
Right arrow Articles by Sawada, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyagi, T.
Right arrow Articles by Sawada, M.

J Biol Chem, Vol. 274, Issue 8, 5004-5011, February 19, 1999

Molecular Cloning and Characterization of a Plasma Membrane-associated Sialidase Specific for Gangliosides

Taeko Miyagi, Tadashi Wada, Akihiro Iwamatsu§, Keiko Hata, Yuko Yoshikawa, Satoru Tokuyama, and Masashi Sawada

From the Division of Biochemistry, Research Institute, Miyagi Prefectural Cancer Center, Natori, Miyagi, 981-1293, Japan and § the Central Laboratories for Key Technology, Kirin Brewery Co. Ltd., Yokohama 236, Japan

Gangliosides are plasma membrane components thought to play important roles in cell surface interactions, cell differentiation, and transmembrane signaling. A mammalian sialidase located in plasma membranes is unique in specifically hydrolyzing gangliosides, suggesting crucial roles in regulation of cell surface functions. Here we describe the cloning and expression of a cDNA for the ganglioside sialidase, isolated from a bovine brain cDNA library based on the amino acid sequence of the purified enzyme from bovine brain. This cDNA encodes a 428-amino acid protein containing a putative transmembrane domain and the three Asp boxes characteristic of sialidases and sharing 19-38% sequence identity with other sialidases. Northern blot and polymerase chain reaction analyses revealed a general distribution of the gene in mammalian species, including man, and the mouse. In COS-7 cells transiently expressing the sialidase, the activity was found to be 40-fold that of the control level with ganglioside substrates in the presence of Triton X-100, and the hydrolysis was almost specific to gangliosides other than GM1 and GM2, both alpha 2right-arrow3 and alpha 2right-arrow8 sialyl linkages being susceptible. The major subcellular localization of the expressed sialidase was assessed to be plasma membrane by Percoll density gradient centrifugation of cell homogenates and by immunofluorescence staining of the transfected COS-7 cells. Analysis of the membrane topology by protease protection assay suggested that this sialidase has a type I membrane orientation with its amino terminus facing to the extracytoplasmic side and lacking a signal sequence.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 J BiochemHome page
R. Kannappan, Y. Satoh, N. Iriyama, M. Ando, M. T. Sawada, N. Takahashi, K. Furuhata, and Y. Uda
Identification and Characterization of Cathepsin D in a Highly Purified Sialidase from Starfish A. pectinifera
J. Biochem., January 1, 2008; 143(1): 117 - 122.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. L. Moore, M. H. Chi, W. Zhou, K. Goleniewska, J. F. O'Neal, J. N. Higginbotham, and R. S. Peebles Jr.
Cutting Edge: Oseltamivir Decreases T Cell GM1 Expression and Inhibits Clearance of Respiratory Syncytial Virus: Potential Role of Endogenous Sialidase in Antiviral Immunity
J. Immunol., March 1, 2007; 178(5): 2651 - 2654.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. C. N. Chiu, M.-K. Chung, R. Koistinen, H. Koistinen, M. Seppala, P.-C. Ho, E. H. Y. Ng, K.-F. Lee, and W. S. B. Yeung
Cumulus Oophorus-associated Glycodelin-C Displaces Sperm-bound Glycodelin-A and -F and Stimulates Spermatozoa-Zona Pellucida Binding
J. Biol. Chem., February 23, 2007; 282(8): 5378 - 5388.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
X. Nan, I. Carubelli, and N. M. Stamatos
Sialidase expression in activated human T lymphocytes influences production of IFN-{gamma}
J. Leukoc. Biol., January 1, 2007; 81(1): 284 - 296.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Hinek, A. V. Pshezhetsky, M. von Itzstein, and B. Starcher
Lysosomal Sialidase (Neuraminidase-1) Is Targeted to the Cell Surface in a Multiprotein Complex That Facilitates Elastic Fiber Assembly
J. Biol. Chem., February 10, 2006; 281(6): 3698 - 3710.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Amado, Q. Yan, E. M. Comelli, B. E. Collins, and J. C. Paulson
Peanut Agglutinin High Phenotype of Activated CD8+ T Cells Results from de Novo Synthesis of CD45 Glycans
J. Biol. Chem., August 27, 2004; 279(35): 36689 - 36697.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Gee, M. Kozlowski, and A. Kumar
Tumor Necrosis Factor-{alpha} Induces Functionally Active Hyaluronan-adhesive CD44 by Activating Sialidase through p38 Mitogen-activated Protein Kinase in Lipopolysaccharide-stimulated Human Monocytic Cells
J. Biol. Chem., September 26, 2003; 278(39): 37275 - 37287.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Sasaki, K. Hata, S. Suzuki, M. Sawada, T. Wada, K. Yamaguchi, M. Obinata, H. Tateno, H. Suzuki, and T. Miyagi
Overexpression of Plasma Membrane-associated Sialidase Attenuates Insulin Signaling in Transgenic Mice
J. Biol. Chem., July 18, 2003; 278(30): 27896 - 27902.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Kakugawa, T. Wada, K. Yamaguchi, H. Yamanami, K. Ouchi, I. Sato, and T. Miyagi
Up-regulation of plasma membrane-associated ganglioside sialidase (Neu3) in human colon cancer and its involvement in apoptosis suppression
PNAS, August 6, 2002; 99(16): 10718 - 10723.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Wang, K. Yamaguchi, T. Wada, K. Hata, X. Zhao, T. Fujimoto, and T. Miyagi
A Close Association of the Ganglioside-specific Sialidase Neu3 with Caveolin in Membrane Microdomains
J. Biol. Chem., July 12, 2002; 277(29): 26252 - 26259.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
N. de Geest, E. Bonten, L. Mann, J. de Sousa-Hitzler, C. Hahn, and A. d'Azzo
Systemic and neurologic abnormalities distinguish the lysosomal disorders sialidosis and galactosialidosis in mice
Hum. Mol. Genet., June 1, 2002; 11(12): 1455 - 1464.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. A. Rodriguez, E. Piddini, T. Hasegawa, T. Miyagi, and C. G. Dotti
Plasma Membrane Ganglioside Sialidase Regulates Axonal Growth and Regeneration in Hippocampal Neurons in Culture
J. Neurosci., November 1, 2001; 21(21): 8387 - 8395.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
Erik. J. Bonten, W. F. Arts, M. Beck, A. Covanis, M. A. Donati, R. Parini, E. Zammarchi, and A. d'Azzo
Novel mutations in lysosomal neuraminidase identify functional domains and determine clinical severity in sialidosis
Hum. Mol. Genet., November 1, 2000; 9(18): 2715 - 2725.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Hasegawa, K. Yamaguchi, T. Wada, A. Takeda, Y. Itoyama, and T. Miyagi
Molecular Cloning of Mouse Ganglioside Sialidase and Its Increased Expression in Neuro2a Cell Differentiation
J. Biol. Chem., March 10, 2000; 275(11): 8007 - 8015.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.