JBC PeproTech; Our Business is Cytokines!

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rahman, I.
Right arrow Articles by MacNee, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rahman, I.
Right arrow Articles by MacNee, W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 8, 5088-5096, February 19, 1999

Molecular Mechanism of the Regulation of Glutathione Synthesis by Tumor Necrosis Factor-alpha and Dexamethasone in Human Alveolar Epithelial Cells

Irfan Rahman, Frank Antonicelli, and William MacNee

From the Rayne Laboratory, Respiratory Medicine Unit, Department of Medicine (RIE), University of Edinburgh, Medical School, Edinburgh Eh8 9AG, United Kingdom

Glutathione (GSH) is an important physiological antioxidant in lung epithelial cells and lung lining fluid. We studied the regulation of GSH synthesis in response to the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha ) and the anti-inflammatory agent dexamethasone in human alveolar epithelial cells (A549). TNF-alpha (10 ng/ml) exposure increased GSH levels, concomitant with a significant increase in gamma -glutamylcysteine synthetase (gamma -GCS) activity and the expression of gamma -GCS heavy subunit (gamma -GCS-HS) mRNA at 24 h. Treatment with TNF-alpha also increased chloramphenicol acetyltransferase (CAT) activity of a gamma -GCS-HS 5'-flanking region reporter construct, transfected into alveolar epithelial cells. Mutation of the putative proximal AP-1-binding site (-269 to -263 base pairs), abolished TNF-alpha -mediated activation of the promoter. Gel shift and supershift analysis showed that TNF-alpha increased AP-1 DNA binding which was predominantly formed by dimers of c-Jun. Dexamethasone (3 µM) produced a significant decrease in the levels of GSH, decreased gamma -GCS activity and gamma -GCS-HS mRNA expression at 24 h. The increase in GSH levels, gamma -GCS-HS mRNA, gamma -GCS-HS promoter activity, and AP-1 DNA binding produced by TNF-alpha were abrogated by co-treating the cells with dexamethasone. Thus these data demonstrate that TNF-alpha and dexamethasone modulate GSH levels and gamma -GCS-HS mRNA expression by their effects on AP-1 (c-Jun homodimer). These data have implications for the oxidant/antioxidant balance in inflammatory lung diseases.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. Rinna and H. J. Forman
SHP-1 Inhibition by 4-Hydroxynonenal Activates Jun N-Terminal Kinase and Glutamate Cysteine Ligase
Am. J. Respir. Cell Mol. Biol., July 1, 2008; 39(1): 97 - 104.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Yao, I. Edirisinghe, S. Rajendrasozhan, S.-R. Yang, S. Caito, D. Adenuga, and I. Rahman
Cigarette smoke-mediated inflammatory and oxidative responses are strain-dependent in mice
Am J Physiol Lung Cell Mol Physiol, June 1, 2008; 294(6): L1174 - L1186.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Kode, S. Rajendrasozhan, S. Caito, S.-R. Yang, I. L. Megson, and I. Rahman
Resveratrol induces glutathione synthesis by activation of Nrf2 and protects against cigarette smoke-mediated oxidative stress in human lung epithelial cells
Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L478 - L488.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. K. Mukherjee, A. K. Mishra, S. Mukhopadhyay, and J. R. Hoidal
High Concentration of Antioxidants N-Acetylcysteine and Mitoquinone-Q Induces Intercellular Adhesion Molecule 1 and Oxidative Stress by Increasing Intracellular Glutathione
J. Immunol., February 1, 2007; 178(3): 1835 - 1844.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Sato, K. Seyama, Y. Sato, H. Mori, S. Souma, T. Akiyoshi, Y. Kodama, T. Mori, S. Goto, K. Takahashi, et al.
Senescence Marker Protein-30 Protects Mice Lungs from Oxidative Stress, Aging, and Smoking
Am. J. Respir. Crit. Care Med., September 1, 2006; 174(5): 530 - 537.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J.-Y. Kim, J.-H. Yim, J.-H. Cho, J.-H. Kim, J.-H. Ko, S.-M. Kim, S. Park, and J.-H. Park
Adrenomedullin Regulates Cellular Glutathione Content via Modulation of {gamma}-Glutamate-Cysteine Ligase Catalytic Subunit Expression
Endocrinology, March 1, 2006; 147(3): 1357 - 1364.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Takamura, N. Fatma, E. Kubo, and D. P. Singh
Regulation of heavy subunit chain of {gamma}-glutamylcysteine synthetase by tumor necrosis factor-{alpha} in lens epithelial cells: role of LEDGF/p75
Am J Physiol Cell Physiol, February 1, 2006; 290(2): C554 - C566.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
C. A. Owen
Proteinases and Oxidants as Targets in the Treatment of Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, November 1, 2005; 2(4): 373 - 385.
[Abstract] [Full Text] [PDF]

Home page
 ERRHome page
W. MacNee
Treatment of stable COPD: antioxidants
Eur. Respir. Rev., September 1, 2005; 14(94): 12 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
W. MacNee
Pulmonary and Systemic Oxidant/Antioxidant Imbalance in Chronic Obstructive Pulmonary Disease
Proceedings of the ATS, April 1, 2005; 2(1): 50 - 60.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. A. Marwick, P. A. Kirkham, C. S. Stevenson, H. Danahay, J. Giddings, K. Butler, K. Donaldson, W. MacNee, and I. Rahman
Cigarette Smoke Alters Chromatin Remodeling and Induces Proinflammatory Genes in Rat Lungs
Am. J. Respir. Cell Mol. Biol., December 1, 2004; 31(6): 633 - 642.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
C. Nara, K. Tateda, T. Matsumoto, A. Ohara, S. Miyazaki, T. J. Standiford, and K. Yamaguchi
Legionella-induced acute lung injury in the setting of hyperoxia: protective role of tumour necrosis factor-{alpha}
J. Med. Microbiol., August 1, 2004; 53(8): 727 - 733.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
D. J. Marlin, L. Johnson, D. A. Kingston, N. C. Smith, C. M. Deaton, S. Mann, P. Heaton, F. Van Vugt, K. Saunders, J. Kydd, et al.
Application of the Comet Assay for Investigation of Oxidative DNA Damage in Equine Peripheral Blood Mononuclear Cells
J. Nutr., August 1, 2004; 134(8): 2133S - 2140S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
P. S. Gilmour, I. Rahman, K. Donaldson, and W. MacNee
Histone acetylation regulates epithelial IL-8 release mediated by oxidative stress from environmental particles
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L533 - L540.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
R Nadif, A Jedlicka, M Mintz, J-P Bertrand, S Kleeberger, and F Kauffmann
Effect of TNF and LTA polymorphisms on biological markers of response to oxidative stimuli in coal miners: a model of gene-environment interaction
J. Med. Genet., February 1, 2003; 40(2): 96 - 103.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
T. Harju, R. Kaarteenaho-Wiik, Y. Soini, R. Sormunen, and V. L. Kinnula
Diminished Immunoreactivity of {gamma}-Glutamylcysteine Synthetase in the Airways of Smokers' Lung
Am. J. Respir. Crit. Care Med., September 1, 2002; 166(5): 754 - 759.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I. Rahman, A. A. M. van Schadewijk, A. J. L. Crowther, P. S. Hiemstra, J. Stolk, W. MacNee, and W. I. De Boer
4-Hydroxy-2-Nonenal, a Specific Lipid Peroxidation Product, Is Elevated in Lungs of Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., August 15, 2002; 166(4): 490 - 495.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
O. L. Miakotina and J. M. Snyder
TNF-alpha inhibits SP-A gene expression in lung epithelial cells via p38 MAPK
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L418 - L427.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Jardine, W. MacNee, K. Donaldson, and I. Rahman
Molecular Mechanism of Transforming Growth Factor (TGF)-beta 1-induced Glutathione Depletion in Alveolar Epithelial Cells. INVOLVEMENT OF AP-1/ARE AND Fra-1
J. Biol. Chem., June 7, 2002; 277(24): 21158 - 21166.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
S. A. A. COMHAIR, P. R. BHATHENA, C. FARVER, F. B. J. M. THUNNISSEN, and S. C. ERZURUM
Extracellular glutathione peroxidase induction in asthmatic lungs: evidence for redox regulation of expression in human airway epithelial cells
FASEB J, January 1, 2001; 15(1): 70 - 78.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
A.G. Shepherd, M. M. Manson, H. W.L. Ball, and L. I. McLellan
Regulation of rat glutamate-cysteine ligase ({gamma}-glutamylcysteine synthetase) subunits by chemopreventive agents and in aflatoxin B1-induced preneoplasia
Carcinogenesis, October 1, 2000; 21(10): 1827 - 1834.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
W. MacNee
Oxidants/Antioxidants and COPD
Chest, May 1, 2000; 117(5_suppl_1): 303S - 317S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. Jarvinen, P. Pietarinen-Runtti, K. Linnainmaa, K. O. Raivio, C. M. Krejsa, T. Kavanagh, and V. L. Kinnula
Antioxidant defense mechanisms of human mesothelioma and lung adenocarcinoma cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2000; 278(4): L696 - L702.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
I. Rahman and W. MacNee
Lung glutathione and oxidative stress: implications in cigarette smoke-induced airway disease
Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1067 - L1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
W. MACNEE and I. RAHMAN
Oxidants and Antioxidants as Therapeutic Targets in Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., November 1, 1999; 160(5): S58 - 65.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.