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J Biol Chem, Vol. 274, Issue 9, 5436-5442, February 26, 1999

Novel Proteoglycan Linkage Tetrasaccharides of Human Urinary Soluble Thrombomodulin, SO4-3GlcAbeta 1-3Galbeta 1-3(±Siaalpha 2-6)Galbeta 1-4Xyl

Hiroyuki WakabayashiDagger , Shunji NatsukaDagger , Tomohiro MegaDagger , Naoki OtsukiDagger , Mitsuko Isajiparallel , Masaaki Naotsuka**, Sadatoshi Koyama**, Toshinori Kanamori**, Kiyoshi Sakai**, and Sumihiro HaseDagger

From the Dagger  Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, ** Pharmaceutical Laboratory, Mochida Pharmaceutical Co., Ltd., Fujieda, Shizuoka 426-8640, and parallel  Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd., Kita-ku, Tokyo 115-8515, Japan

O-linked sugar chains with xylose as a reducing end linked to human urinary soluble thrombomodulin were studied. Sugar chains were liberated by hydrazinolysis followed by N-acetylation and tagged with 2-aminopyridine. Two fractions containing pyridylaminated Xyl as a reducing end were collected. Their structures were determined by partial acid hydrolysis, two-dimensional sugar mapping combined with exoglycosidase digestions, methylation analysis, mass spectrometry, and NMR as SO4-3GlcAbeta 1-3Galbeta 1-3(±Siaalpha 2-6)Galbeta 1-4Xyl. These sugar chains could bind to an HNK-1 monoclonal antibody. This is believed to be the first example of a proteoglycan linkage tetrasaccharide with glucuronic acid 3-sulfate and sialic acid.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.


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Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.