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J Biol Chem, Vol. 274, Issue 9, 5654-5658, February 26, 1999
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From the Departments of Cytochrome c is released from
mitochondria into the cytosol in cells undergoing apoptosis. The
temporal relationship between cytochrome c release and loss
of mitochondrial membrane potential was monitored by laser-scanning
confocal microscopy in single living pheochromocytoma-6 cells
undergoing apoptosis induced by staurosporine. Mitochondrial membrane
potential monitored by tetramethylrhodamine methyl ester decreased
abruptly in individual cells from 2 to 7 h after treatment with
staurosporine. Depolarization was accompanied by cytochrome
c release documented by release of transfected green fluorescent protein-tagged cytochrome c in these cells. The
results show that mitochondrial depolarization accompanies cytochrome c release in pheochromocytoma-6 cells undergoing apoptosis.
Anatomy, ¶ Physiology
and Biophysics, and Biomedical Engineering, School of Medicine,
Case Western Reserve University, Cleveland, Ohio 44106
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L. He, A. T. Poblenz, C. J. Medrano, and D. A. Fox Lead and Calcium Produce Rod Photoreceptor Cell Apoptosis by Opening the Mitochondrial Permeability Transition Pore J. Biol. Chem., April 14, 2000; 275(16): 12175 - 12184. [Abstract] [Full Text] [PDF]
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L. Scorrano, V. Petronilli, R. Colonna, F. Di Lisa, and P. Bernardi Chloromethyltetramethylrosamine (Mitotracker OrangeTM) Induces the Mitochondrial Permeability Transition and Inhibits Respiratory Complex I. IMPLICATIONS FOR THE MECHANISM OF CYTOCHROME c RELEASE J. Biol. Chem., August 27, 1999; 274(35): 24657 - 24663. [Abstract] [Full Text] [PDF]
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L. SCORRANO, V. PETRONILLI, R. COLLONA, F. DI LISA, and P. BERNARDI Interactions of Chloromethyltetramethylrosamine (Mitotracker OrangeTM) with Isolated Mitochondria and Intact Cells Ann. N.Y. Acad. Sci., January 1, 1999; 893(1): 391 - 395. [Full Text] [PDF]
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A. A. Matassa, L. Carpenter, T. J. Biden, M. J. Humphries, and M. E. Reyland PKCdelta Is Required for Mitochondrial-dependent Apoptosis in Salivary Epithelial Cells J. Biol. Chem., August 3, 2001; 276(32): 29719 - 29728. [Abstract] [Full Text] [PDF]
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Z. Pan, D. Damron, A.-L. Nieminen, M. B. Bhat, and J. Ma Depletion of Intracellular Ca2+ by Caffeine and Ryanodine Induces Apoptosis of Chinese Hamster Ovary Cells Transfected with Ryanodine Receptor J. Biol. Chem., June 23, 2000; 275(26): 19978 - 19984. [Abstract] [Full Text] [PDF]
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H. Oubrahim, E. R. Stadtman, and P. B. Chock Mitochondria play no roles in Mn(II)-induced apoptosis in HeLa cells PNAS, August 14, 2001; 98(17): 9505 - 9510. [Abstract] [Full Text] [PDF]
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G. Feng and N. Kaplowitz Mechanism of staurosporine-induced apoptosis in murine hepatocytes Am J Physiol Gastrointest Liver Physiol, May 1, 2002; 282(5): G825 - G834. [Abstract] [Full Text] [PDF]
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