JBC Anatrace, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Armah, D. A.
Right arrow Articles by Mensa-Wilmot, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Armah, D. A.
Right arrow Articles by Mensa-Wilmot, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 274, Issue 9, 5931-5938, February 26, 1999

S-Myristoylation of a Glycosylphosphatidylinositol-specific Phospholipase C in Trypanosoma brucei

Dora Abena Armah and Kojo Mensa-Wilmot

From the Department of Cellular Biology, University of Georgia, Athens, Georgia 30602

Covalent modification with lipid can target cytosolic proteins to biological membranes. With intrinsic membrane proteins, the role of acylation can be elusive. Herein, we describe covalent lipid modification of an integral membrane glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC) from the kinetoplastid Trypanosoma brucei. Myristic acid was detected on cysteine residue(s) (i.e. thiomyristoylation). Thiomyristoylation occurred both co- and post-translationally. Acylated GPI-PLC was active against variant surface glycoprotein (VSG). The half-life of fatty acid on GPI-PLC was 45 min, signifying the dynamic nature of the modification. Deacylation in vitro decreased activity of GPI-PLC 18-30-fold. Thioacylation, from kinetic analysis, activated GPI-PLC by accelerating the conversion of a GPI-PLC·VSG complex to product. Reversible thioacylation is a novel mechanism for regulating the activity of a phospholipase C.

Copyright © 1999 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
D. D. O. Martin, G. L. Vilas, J. A. Prescher, G. Rajaiah, J. R. Falck, C. R. Bertozzi, and L. G. Berthiaume
Rapid detection, discovery, and identification of post-translationally myristoylated proteins during apoptosis using a bio-orthogonal azidomyristate analog
FASEB J, March 1, 2008; 22(3): 797 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. L. Vilas, M. M. Corvi, G. J. Plummer, A. M. Seime, G. R. Lambkin, and L. G. Berthiaume
Posttranslational myristoylation of caspase-activated p21-activated protein kinase 2 (PAK2) potentiates late apoptotic events
PNAS, April 25, 2006; 103(17): 6542 - 6547.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Zheng, K. D. Butler, R. K. Tweten, and K. Mensa-Wilmot
Endosomes, Glycosomes, and Glycosylphosphatidylinositol Catabolism in Leishmania major
J. Biol. Chem., October 1, 2004; 279(40): 42106 - 42113.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. A. Corcoran and R. Duncan
Reptilian Reovirus Utilizes a Small Type III Protein with an External Myristylated Amino Terminus To Mediate Cell-Cell Fusion
J. Virol., April 15, 2004; 78(8): 4342 - 4351.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. J. McConville, K. A. Mullin, S. C. Ilgoutz, and R. D. Teasdale
Secretory Pathway of Trypanosomatid Parasites
Microbiol. Mol. Biol. Rev., March 1, 2002; 66(1): 122 - 154.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Paturiaux-Hanocq, J. Hanocq-Quertier, M. L. C. de Almeida, D. P. Nolan, A. Pays, L. Vanhamme, J. Van den Abbeele, C. L. Wasunna, M. Carrington, and E. Pays
A Role for the Dynamic Acylation of a Cluster of Cysteine Residues in Regulating the Activity of the Glycosylphosphatidylinositol-specific Phospholipase C of Trypanosoma brucei
J. Biol. Chem., April 14, 2000; 275(16): 12147 - 12155.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Furuya, C. Kashuba, R. Docampo, and S. N. J. Moreno
A Novel Phosphatidylinositol-Phospholipase C of Trypanosoma cruzi That Is Lipid Modified and Activated during Trypomastigote to Amastigote Differentiation
J. Biol. Chem., February 25, 2000; 275(9): 6428 - 6438.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Cardoso De Almeida, M Geuskens, and E Pays
Cell lysis induces redistribution of the GPI-anchored variant surface glycoprotein on both faces of the plasma membrane of Trypanosoma brucei
J. Cell Sci., January 12, 1999; 112(23): 4461 - 4473.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Armah and K. Mensa-Wilmot
Tetramerization of Glycosylphosphatidylinositol-specific Phospholipase C from Trypanosoma brucei
J. Biol. Chem., June 16, 2000; 275(25): 19334 - 19342.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
F. Agüero, R. E. Verdún, A. C. C. Frasch, and D. O. Sánchez
A Random Sequencing Approach for the Analysis of the Trypanosoma cruzi Genome: General Structure, Large Gene and Repetitive DNA Families, and Gene Discovery
Genome Res., December 1, 2000; 10(12): 1996 - 2005.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 1999 by the American Society for Biochemistry and Molecular Biology.