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J Biol Chem, Vol. 275, Issue 1, 451-460, January 7, 2000

The Synaptic Vesicle Protein SV2 Is Complexed with an 5-Containing Laminin on the Nerve Terminal Surface^*

Young-Jin Son, Todd W. Scranton, William J. Sunderland, Sung J. Baek, Jeffrey H. Miner, Joshua R. Sanes^§, and Steven S. Carlson^¶

From the Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195-7290 and Departments of  Medicine and of ^§ Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110

Interactions between growing axons and synaptic basal lamina components direct the formation of neuromuscular junctions during nerve regeneration. Isoforms of laminin containing 5 or 2 chains are potential basal lamina ligands for these interactions. The nerve terminal receptors are unknown. Here we show that SV2, a synaptic vesicle transmembrane proteoglycan, is complexed with a 900-kDa laminin on synaptosomes from the electric organ synapse that is similar to the neuromuscular junctions. Although two laminins are present on synaptosomes, only the 900-kDa laminin is associated with SV2. Other nerve terminal components are absent from this complex. The 900-kDa laminin contains an 5, a 1, and a novel  chain. To test whether SV2 directly binds the 900-kDa laminin, we looked for interaction between purified SV2 and laminin-1, a laminin isoform with a similar structure. We find SV2 binds with high affinity to purified laminin-1. Our results suggest that a synaptic vesicle component may act as a laminin receptor on the presynaptic plasma membrane; they also suggest a mechanism for activity-dependent adhesion at the synapse.

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