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J Biol Chem, Vol. 275, Issue 1, 487-496, January 7, 2000
From the Metabolism Branch, Division of Clinical Sciences, NCI,
National Institutes of Health, Bethesda, Maryland 20892
The immediate-early IE62 protein of varicella
zoster virus is an acidic transcriptional activator capable of
up-regulating many viral and cellular promoters with varying
efficiencies. We demonstrate that, in the context of a minimal
promoter, a TATA element is both sufficient and essential for
IE62-mediated transcriptional activation. Differential levels of
activation by IE62 in this context were conferred by a panel of
naturally occurring sequence variations within the TATA motif itself.
TATA motif-specific, differential induction was not obtained when the
IE62 acidic activation domain was targeted as a GAL4 fusion protein to
the same panel. The prototype acidic transactivator, VP16 of herpes
simplex virus, failed to discriminate between these different TATA
motifs when they were placed into an appropriate responsive promoter
context. Nonetheless, a chimeric IE62 polypeptide substituted with the VP16 activation domain retained the ability to differentially modulate
minimal promoters with various TATA motifs. Taken together with its
binding to TATA box-binding protein (TBP) and transcription factor IIB
in vitro, we suggest that IE62 has the unusual ability to
achieve differential levels of transcriptional activation through different TATA motifs, which may be accomplished either directly or
indirectly by recognizing conformational variations in DNA-bound TBP,
TBP-transcription factor IIA/B, or TBP-TATA-associated factor complexes.
The TATA Motif Specifies the Differential Activation of
Minimal Promoters by Varicella Zoster Virus Immediate-early Regulatory
Protein IE62*
*
This work was supported in part by National Institutes of
Health NIAID Intramural Project AI 00687, of which L. P. P was the principal investigator.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Bldg. 10, Rm. 4B40,
Metabolism Branch, Division of Clinical Sciences, 10 Center Dr., MSC
1374, NCI, National Institutes of Health, Bethesda, MD 20892-1374. Tel.: 301-435-7518; Fax: 301-496-9956; E-mail:
lperera@niaid.nih.gov.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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