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J Biol Chem, Vol. 275, Issue 1, 697-704, January 7, 2000
From the E. C. Slater Instituut, BioCentrum Amsterdam,
University of Amsterdam, Plantage Muidergracht 12, 1018 TV Amsterdam, The Netherlands
Polycomb group (PcG) proteins repress gene
activity over a considerable distance, possibly by spreading along the
chromatin fiber. Insulators or boundary elements, genetic elements
within the chromatin, may serve to terminate the repressing action of PcG proteins. We studied the ability of insulators to block the action
of chromatin-associated repressors such as PcG proteins, HP1, and
MeCP2. We found that the Drosophila special chromatin structure insulator completely blocks transcriptional repression mediated by all of the repressors we tested. The Drosophila
gypsy insulator was able to block the repression mediated by the PcG proteins Su(z)2 and RING1, as well as mHP1, but not the repression mediated by MeCP2 and the PcG protein HPC2. The 5'-located DNase I-hypersensitive site in the chicken
Transcriptional Repression Mediated by Polycomb Group
Proteins and Other Chromatin-associated Repressors Is Selectively
Blocked by Insulators*
,
-globin locus displayed a
limited ability to block repression, and a matrix or scaffold attachment region element was entirely unable to block repression mediated by any repressor tested. Our results indicate that insulators can block repression mediated by PcG proteins and other
chromatin-associated repressors, but with a high level of selectivity.
This high degree of specificity may provide a useful assay to define
and characterize distinct classes of insulators.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by a grant from Stichting Technische Wetenschappen.
§
Supported by a grant from Nederlandse Organisatie voor
Wetenschappel
k Onderzok.
¶
To whom correspondence should be addressed. Tel.:
31-20-5255115; Fax: 31-20-5255124; E-mail:
arie.otte@chem.uva.nl.
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