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J Biol Chem, Vol. 275, Issue 10, 7013-7020, March 10, 2000
From the Freie Universität Berlin, Institut für
Kristallographie, Takustrasse 6, D-14195 Berlin, Germany
The interaction of the two transcriptional
regulators RcsA and RcsB with a specific operator is a common mechanism
in the activation of capsule biosynthesis in enteric bacteria. We
describe RcsAB binding sites in the wza promoter of the
operon for colanic acid biosynthesis in Escherichia coli
K-12, in the galF promoter of the operon for K2 antigen
biosynthesis in Klebsiella pneumoniae, and in the
tviA (vipR) promoter of the operon for Vi
antigen biosynthesis in Salmonella typhi. We further show
the interaction of RcsAB with the rcsA promoters of various
species, indicating that rcsA autoregulation also depends on the
presence of both proteins. The compilation of all identified RcsAB
binding sites revealed the conserved core sequence TaAGaatatTCctA,
which we propose to be termed RcsAB box. The RcsAB box is also part of
Bordetella pertussis BvgA binding sites and may represent a
more distributed recognition motif within the LuxR superfamily of
transcriptional regulators. The RcsAB box is essential for the
induction of Rcs-regulated promoters. Site-specific mutations of
conserved nucleotides in the RcsAB boxes of the E. coli wza
and rcsA promoters resulted in an
exopolysaccharide-negative phenotype and in the reduction of reporter
gene expression.
To whom correspondence should be addressed. Tel.: 49-30-838-3463;
Fax: 49-30-838-6702; E-mail: fbern@chemie.fu-berlin.de.
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