Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lai, J.-F.
Right arrow Articles by Chen, H.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lai, J.-F.
Right arrow Articles by Chen, H.-C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 11, 7474-7480, March 17, 2000

Involvement of Focal Adhesion Kinase in Hepatocyte Growth Factor-induced Scatter of Madin-Darby Canine Kidney Cells*

Jui-Fen LaiDagger , Shu-Chen KaoDagger , Si-Tse Jiang§, Ming-Jer Tang§, Po-Chao ChanDagger , and Hong-Chen ChenDagger

From the Dagger  Department of Zoology, National Chung Hsing University, Taichung 40227, Taiwan and the § Department of Physiology, National Cheng Kung University Medical College, Tainan 70101, Taiwan, Republic of China

Focal adhesion kinase (FAK) has been implicated to play a critical role in integrin-mediated control of cell behavior. However, it is unclear whether FAK also participates in the regulation of growth factor-elicited cellular functions. In this study, we have demonstrated that although overexpression of FAK in Madin-Dardy canine kidney cells did not alter their growth property or ability to form tubules within collagen gel upon hepatocyte growth factor (HGF) stimulation, it apparently enhanced HGF-induced cell scattering. This enhancement was largely because of an increase in the third phase (i.e. cell migration) of cell scattering rather than the first two phases (i.e. cell spreading and cell-cell dissociation). Conversely, the expression of FAK-related nonkinase significantly (~60%) inhibited HGF-induced cell migration. Moreover, we have found that the effect of FAK on promoting HGF-induced cell motility was greatly dependent on cell-matrix interactions. We showed that HGF treatment selectively increased the expression of integrins alpha 2 and, to a lesser extent, alpha 3 in Madin-Dardy canine kidney cells and that a monoclonal antibody against integrin alpha 2 efficiently blocked HGF-enhanced cell migration on collagen. In our efforts to determine the mechanism by which FAK promotes HGF-induced cell migration, we found that FAK mutants deficient in phosphatidylinositol 3-kinase or p130Cas binding failed to promote HGF-induced cell migration. Interestingly, cells expressing a FAK mutant defective in Grb2 binding exhibited a rate of migration ~50% lower than that of cells expressing wild type FAK in response to HGF stimulation. Taken together, our results suggest a link between HGF-increased integrin expression, FAK activation, and enhanced cell motility and implicate a role for FAK in the facilitation of growth factor-induced cell motility.

* This work was supported by National Science Council, Taiwan, Grant NSC89-2311-B005-031 (to H.-C. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed. Tel.: 886-4-2854922; Fax: 886-4-2851797; E-mail: hcchen@nchu.edu.tw.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. V. Volpe, E. Chung, J. P. Ulm, B. F. Gilchrist, S. Ralston, K. T. Wang, and H. C. Nielsen
Aberrant cell adhesion molecule expression in human bronchopulmonary sequestration and congenital cystic adenomatoid malformation
Am J Physiol Lung Cell Mol Physiol, July 1, 2009; 297(1): L143 - L152.
[Abstract] [Full Text] [PDF]

Home page
 Anticancer ResHome page
F. KOGA, K. KIHARA, and L. NECKERS
Inhibition of Cancer Invasion and Metastasis by Targeting the Molecular Chaperone Heat-shock Protein 90
Anticancer Res, March 1, 2009; 29(3): 797 - 807.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C.-L. Chen and H.-C. Chen
Functional suppression of E-cadherin by protein kinase C{delta}
J. Cell Sci., February 15, 2009; 122(4): 513 - 523.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. Wang, M. Zheng, G. Liu, W. Xia, P. J. McKeown-Longo, M.-C. Hung, and J. Zhao
Kruppel-Like Factor 8 Induces Epithelial to Mesenchymal Transition and Epithelial Cell Invasion
Cancer Res., August 1, 2007; 67(15): 7184 - 7193.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C.-L. Chen, Y.-T. Hsieh, and H.-C. Chen
Phosphorylation of adducin by protein kinase C{delta} promotes cell motility
J. Cell Sci., April 1, 2007; 120(7): 1157 - 1167.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S.-Y. Chen and H.-C. Chen
Direct Interaction of Focal Adhesion Kinase (FAK) with Met Is Required for FAK To Promote Hepatocyte Growth Factor-Induced Cell Invasion.
Mol. Cell. Biol., July 1, 2006; 26(13): 5155 - 5167.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-C. Wu, T.-Y. Chen, C.-T. R. Yu, S.-J. Tsai, J.-M. Hsu, M.-J. Tang, C.-K. Chou, W.-J. Lin, C.-J. Yuan, and C.-Y. F. Huang
Identification of V23RalA-Ser194 as a Critical Mediator for Aurora-A-induced Cellular Motility and Transformation by Small Pool Expression Screening
J. Biol. Chem., March 11, 2005; 280(10): 9013 - 9022.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Zlot, G. Ingle, J. Hongo, S. Yang, Z. Sheng, R. Schwall, N. Paoni, F. Wang, F. V. Peale Jr., and M. E. Gerritsen
Stanniocalcin 1 Is an Autocrine Modulator of Endothelial Angiogenic Responses to Hepatocyte Growth Factor
J. Biol. Chem., November 28, 2003; 278(48): 47654 - 47659.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
H. Ma, T. M. Calderon, T. Kessel, A. W. Ashton, and J. W. Berman
Mechanisms of Hepatocyte Growth Factor-Mediated Vascular Smooth Muscle Cell Migration
Circ. Res., November 28, 2003; 93(11): 1066 - 1073.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-K. Wang, Y.-H. Wang, C.-Z. Wang, J.-M. Sung, W.-T. Chiu, S.-H. Lin, Y.-H. Chang, and M.-J. Tang
Rigidity of Collagen Fibrils Controls Collagen Gel-induced Down-regulation of Focal Adhesion Complex Proteins Mediated by {alpha}2{beta}1 Integrin
J. Biol. Chem., June 6, 2003; 278(24): 21886 - 21892.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Mareel and A. Leroy
Clinical, Cellular, and Molecular Aspects of Cancer Invasion
Physiol Rev, April 1, 2003; 83(2): 337 - 376.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Corredor, F. Yan, C. C. Shen, W. Tong, S. K. John, G. Wilson, R. Whitehead, and D. B. Polk
Tumor necrosis factor regulates intestinal epithelial cell migration by receptor-dependent mechanisms
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C953 - C961.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
E. H. Van Aken, O. De Wever, L. Van Hoorde, E. Bruyneel, J.-J. De Laey, and M. M. Mareel
Invasion of Retinal Pigment Epithelial Cells: N-cadherin, Hepatocyte Growth Factor, and Focal Adhesion Kinase
Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 463 - 472.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P.-C. Chan, C.-C. Liang, K.-C. Yu, M.-C. Chang, W. L. Ho, B.-H. Chen, and H.-C. Chen
Synergistic Effect of Focal Adhesion Kinase Overexpression and Hepatocyte Growth Factor Stimulation on Cell Transformation
J. Biol. Chem., December 20, 2002; 277(52): 50373 - 50379.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Abdel-Ghany, H.-C. Cheng, R. C. Elble, and B. U. Pauli
Focal Adhesion Kinase Activated by beta 4 Integrin Ligation to mCLCA1 Mediates Early Metastatic Growth
J. Biol. Chem., September 6, 2002; 277(37): 34391 - 34400.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
B. Dekel, N. Amariglio, N. Kaminski, A. Schwartz, E. Goshen, F. D. Arditti, I. Tsarfaty, J. H. Passwell, Y. Reisner, and G. Rechavi
Engraftment and Differentiation of Human Metanephroi into Functional Mature Nephrons after Transplantation into Mice Is Accompanied by a Profile of Gene Expression Similar to Normal Human Kidney Development
J. Am. Soc. Nephrol., April 1, 2002; 13(4): 977 - 990.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z.-X. Liu, C. F. Yu, C. Nickel, S. Thomas, and L. G. Cantley
Hepatocyte Growth Factor Induces ERK-dependent Paxillin Phosphorylation and Regulates Paxillin-Focal Adhesion Kinase Association
J. Biol. Chem., March 15, 2002; 277(12): 10452 - 10458.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Sano, M. Kira, S. Takagi, K. Yoshikawa, J. Takeda, and S. Itami
Two distinct signaling pathways in hair cycle induction: Stat3-dependent and -independent pathways
PNAS, November 16, 2000; (2000) 240303097.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. R. Hauck, D. A. Hsia, and D. D. Schlaepfer
Focal Adhesion Kinase Facilitates Platelet-derived Growth Factor-BB-stimulated ERK2 Activation Required for Chemotaxis Migration of Vascular Smooth Muscle Cells
J. Biol. Chem., December 22, 2000; 275(52): 41092 - 41099.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-C. Liang and H.-C. Chen
Sustained Activation of Extracellular Signal-regulated Kinase Stimulated by Hepatocyte Growth Factor Leads to Integrin alpha 2 Expression That Is Involved in Cell Scattering
J. Biol. Chem., June 8, 2001; 276(24): 21146 - 21152.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Sano, M. Kira, S. Takagi, K. Yoshikawa, J. Takeda, and S. Itami
Two distinct signaling pathways in hair cycle induction: Stat3-dependent and -independent pathways
PNAS, December 5, 2000; 97(25): 13824 - 13829.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement