|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 11, 7612-7618, March 17, 2000
From the Glucose depletion derepresses the
Saccharomyces cerevisiae ADH2 gene; this metabolic change
is accompanied by chromatin structural modifications in the promoter
region. We show that the ADR6/SWI1 gene is not
necessary for derepression of the wild type chromosomal ADH2, whereas the transcription factor Adr1p, which
regulates several S. cerevisiae functions, plays a major
role in driving nucleosome reconfiguration and ADH2
expression. When we tested the effect of individual domains of the
regulatory protein Adr1p on the chromatin structure of
ADH2, a remodeling consisting of at least two steps was
observed. Adr1p derivatives were analyzed in derepressing conditions,
showing that the Adr1p DNA binding domain alone causes an alteration in
chromatin organization in the absence of transcription. This alteration
differs from the remodeling observed in the presence of the Adr1p
activation domain when the promoter is transcriptionally active.
Two Distinct Nucleosome Alterations Characterize Chromatin
Remodeling at the Saccharomyces cerevisiae ADH2
Promoter*
§,
Centro di Studio per gli Acidi Nucleici,
Consiglio Nazionale delle Ricerche, the § Fondazione
Istituto Pasteur-Fondazione Cenci Bolognetti, c/o Dipartimento di
Genetica e Biologia Molecolare, and the ¶ Dipartimento di Scienze
Biochimiche, Università "La Sapienza," P.le Aldo Moro 5, 00185 Rome, Italy
*
This work was supported by grants from the G. P. Ateneo
"La Sapienza," the MURST project "Protein-Nucleic Acid
Interactions," and the Consiglio Nazionale delle Ricerche Target
Project on Biotechnology.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
39-06-49912659; Fax: 39-06-49912500; E-mail:
Caserta@axrma.uniroma1.it.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Govender, J. L. Domingo, M. C. Bester, I. S. Pretorius, and F. F. Bauer Controlled Expression of the Dominant Flocculation Genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae Appl. Envir. Microbiol., October 1, 2008; 74(19): 6041 - 6052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. T. Young, C. Tachibana, H.-W. E. Chang, K. M. Dombek, E. M. Arms, and R. Biddick Artificial Recruitment of Mediator by the DNA-Binding Domain of Adr1 Overcomes Glucose Repression of ADH2 Expression Mol. Cell. Biol., April 15, 2008; 28(8): 2509 - 2516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tachibana, J. Y. Yoo, J.-B. Tagne, N. Kacherovsky, T. I. Lee, and E. T. Young Combined Global Localization Analysis and Transcriptome Data Identify Genes That Are Directly Coregulated by Adr1 and Cat8 Mol. Cell. Biol., March 15, 2005; 25(6): 2138 - 2146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Di Mauro, L. Verdone, B. Chiappini, and M. Caserta In Vivo Changes of Nucleosome Positioning in the Pretranscription State J. Biol. Chem., February 22, 2002; 277(9): 7002 - 7009. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Stafford and R. H. Morse GCN5 Dependence of Chromatin Remodeling and Transcriptional Activation by the GAL4 and VP16 Activation Domains in Budding Yeast Mol. Cell. Biol., July 15, 2001; 21(14): 4568 - 4578. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |