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J Biol Chem, Vol. 275, Issue 11, 7731-7742, March 17, 2000
From the Murine sperm initiate fertilization by binding to
specific oligosaccharides linked to the zona pellucida, the specialized matrix coating the egg. Biophysical analyses have revealed the presence
of both high mannose and complex-type N-glycans in murine zona pellucida. The predominant high mannose-type glycan had the composition Man5GlcNAc2, but larger
oligosaccharides of this type were also detected. Biantennary,
triantennary, and tetraantennary complex-type N-glycans
were found to be terminated with the following antennae:
Gal
Structural Analysis of Murine Zona Pellucida Glycans
EVIDENCE FOR THE EXPRESSION OF CORE 2-TYPE O-GLYCANS
AND THE Sda ANTIGEN*
,¶,¶
Department of Biochemistry, Imperial College
of Science, Technology and Medicine, London SW7 2AY, United Kingdom and
§ Department of Physiological Sciences, Eastern Virginia
Medical School, Norfolk, Virginia 23501
1-4GlcNAc, NeuAc
2-3Gal1-4GlcNAc,
NeuGc2-3Gal1-4GlcNAc, the Sda antigen
(NeuAc2-3[GalNAc1-4]Gal1-4GlcNAc,
NeuGc2-3[GalNAc1-4]Gal1-4GlcNAc), and terminal GlcNAc.
Polylactosamine-type sequence was also detected on a subset of the
antennae. Analysis of the O-glycans indicated that the
majority were core 2-type
(Gal1-4GlcNAc1-6[Gal1-3]GalNAc). The 1-6-linked
branches attached to these O-glycans were terminated with
the same sequences as the N-glycans, except for terminal GlcNAc. Glycans bearing Gal1-4GlcNAc1-6 branches have
previously been suggested to mediate initial murine gamete binding.
Oligosaccharides terminated with GalNAc1-4Gal have been implicated
in the secondary binding interaction that occurs following the acrosome
reaction. The significant implications of these observations are discussed.
*
This work was supported by a grant from the Biotechnology
and Biological Sciences Research Council (to H. R. M. and A. D.) and
a grant from the Wellcome Trust (to H. R. M. and A. D.). This study
was also supported by the Jeffress Trust (to G. F. C.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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