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J Biol Chem, Vol. 275, Issue 11, 7994-7999, March 17, 2000
From the Department of Pharmacology and Cancer Biology, Duke
University Medical Center, Durham, North Carolina 27710
Ca2+/calmodulin-dependent
protein kinase IV and calspermin are two proteins encoded by the
Camk4 gene. Both are highly expressed in the testis, where
in situ hybridization studies in rat testes have
demonstrated that CaMKIV mRNA is localized to pachytene
spermatocytes, while calspermin mRNA is restricted to spermatids.
We have examined the expression patterns of both CaMKIV and calspermin
in mouse testis and unexpectedly find that CaMKIV is expressed in
spermatogonia and spermatids but excluded from spermatocytes, while
calspermin is found only in spermatids. CaMKIV and calspermin
expression in the testis are stage-dependent and appear to
be coordinately regulated. In germ cells, we find that CaMKIV is
associated with the chromatin. We further demonstrate that a fraction
of CaMKIV in spermatids is hyperphosphorylated and specifically
localized to the nuclear matrix. These novel findings may implicate
CaMKIV in chromatin remodeling during nuclear condensation of spermatids.
Ca2+/Calmodulin-dependent Protein Kinase
IV Is Expressed in Spermatids and Targeted to Chromatin and the Nuclear
Matrix*
*
This work was supported by a National Institutes of Health
(NIH) Medical Scientist Training Program award (to J. Y. W.) and NIH
Grant HD07503 (to A. R. M.).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 919-681-6209;
Fax: 919-681-8461; E-mail: means001@mc.duke.edu.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
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