HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kang, J. S. Articles by Kim, J.-S. Search for Related Content PubMed PubMed Citation Articles by Kang, J. S. Articles by Kim, J.-S. Social Bookmarking                      What's this?

J Biol Chem, Vol. 275, Issue 12, 8742-8748, March 24, 2000

Zinc Finger Proteins as Designer Transcription Factors^*

Jong Seok Kang and Jin-Soo Kim^§¶

From the  Samsung Biomedical Research Institute and ^§ Toolgen Incorporation, Sungkyunkwan University School of Medicine, 300 Chunchun-Dong, Changan-Ku, Suwon, Republic of Korea

Recent progress in the design and selection of novel zinc finger proteins with desired DNA binding specificities now allows construction of tailor-made DNA-binding proteins that specifically recognize almost any predetermined DNA sequence. Such novel or "designer" zinc finger proteins with desired DNA binding specificities can serve as efficient transcription factors in various mammalian cell lines. In addition, they may be broadly useful in the regulation of endogenous genes in transgenic organisms and eventually in gene therapy applications. In this report, we use a series of transient and stable transfection experiments to demonstrate that the expression of a target gene can be controlled by changing the in vivo concentration of designer zinc finger proteins in a dose-dependent manner. We also report that designer zinc finger proteins can access their binding sites integrated into the genome and function as potent transcription factors. Our results suggest that designer zinc finger transcription factors that specifically recognize appropriate sites in the promoter of a target gene may have broad applications in the post-genomic era.

---

^* This work was supported by Grant B98018 from the Samsung Biomedical Research Institute.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

^¶ To whom correspondence should be addressed. E-mail: jinsookim@netsgo.com.

---

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				C.-O. Yun, H.-C. Shin, T.-D. Kim, W.-H. Yoon, Y.-A Kang, H.-S. Kwon, S. K. Kim, and J.-S. Kim Transduction of artificial transcriptional regulatory proteins into human cells Nucleic Acids Res., July 21, 2008; (2008) gkn398v1. [Abstract] [Full Text] [PDF]

				W. Tan, Z. Dong, T. A. Wilkinson, C. F. Barbas III, and S. A. Chow Human Immunodeficiency Virus Type 1 Incorporated with Fusion Proteins Consisting of Integrase and the Designed Polydactyl Zinc Finger Protein E2C Can Bias Integration of Viral DNA into a Predetermined Chromosomal Region in Human Cells J. Virol., February 15, 2006; 80(4): 1939 - 1948. [Abstract] [Full Text] [PDF]

				W. Tan, K. Zhu, D. J. Segal, C. F. Barbas III, and S. A. Chow Fusion Proteins Consisting of Human Immunodeficiency Virus Type 1 Integrase and the Designed Polydactyl Zinc Finger Protein E2C Direct Integration of Viral DNA into Specific Sites J. Virol., February 1, 2004; 78(3): 1301 - 1313. [Abstract] [Full Text] [PDF]

				T. G. Uil, H. J. Haisma, and M. G. Rots Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities Nucleic Acids Res., November 1, 2003; 31(21): 6064 - 6078. [Abstract] [Full Text] [PDF]

				D. Xu, D. Falke, and R. L. Juliano P53-Dependent Cell-Killing by Selective Repression of Thymidine Kinase and Reduced Prodrug Activation Mol. Pharmacol., August 1, 2003; 64(2): 289 - 297. [Abstract] [Full Text] [PDF]

				X. Guan, J. Stege, M. Kim, Z. Dahmani, N. Fan, P. Heifetz, C. F. Barbas III, and S. P. Briggs Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors PNAS, October 1, 2002; 99(20): 13296 - 13301. [Abstract] [Full Text] [PDF]

				D. Xu, D. Ye, M. Fisher, and R. L. Juliano Selective Inhibition of P-glycoprotein Expression in Multidrug-Resistant Tumor Cells by a Designed Transcriptional Regulator J. Pharmacol. Exp. Ther., September 1, 2002; 302(3): 963 - 971. [Abstract] [Full Text] [PDF]

				R.L. Juliano, A. Astriab-Fisher, and D. Falke Macromolecular Therapeutics: Emerging Strategies for Drug Discovery in the Postgenome Era Mol. Interv., April 1, 2001; 1(1): 40 - 53. [Abstract] [Full Text]

				M. Moore, Y. Choo, and A. Klug From the Cover: Design of polyzinc finger peptides with structured linkers PNAS, February 13, 2001; 98(4): 1432 - 1436. [Abstract] [Full Text] [PDF]

				M. E. Laurance, D. B. Starr, E. F. Michelotti, E. Cheung, C. Gonzalez, A. W. Tam, J. Deikman, C. A. Edwards, and A. J. Bardwell Specific down-regulation of an engineered human cyclin D1 promoter by a novel DNA-binding ligand in intact cells Nucleic Acids Res., February 1, 2001; 29(3): 652 - 661. [Abstract] [Full Text] [PDF]