Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Afroze, T.
Right arrow Articles by Husain, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Afroze, T.
Right arrow Articles by Husain, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

J Biol Chem, Vol. 275, Issue 12, 9062-9069, March 24, 2000

c-Myb-binding Sites Mediate G1/S-associated Repression of the Plasma Membrane Ca2+-ATPase-1 Promoter*

Talat Afroze and Mansoor HusainDagger

From the Dagger  Centre for Cardiovascular Research, 3-816, 101 College Street, Toronto General Hospital, Toronto, Ontario M5G 1L5, Canada

We demonstrate that two Myb-binding sites of the mouse plasma membrane Ca2+-ATPase-1 (PMCA1) promoter are required for G1/S cell cycle stage-associated repression of PMCA1 promoter activity. Nuclear run-on experiments revealed G1/S-associated repression of PMCA1 transcription. Ribonuclease protection assays revealed two transcription initiation sites between two Myb-binding sites in the PMCA1 promoter. Gel shift assays showed that c-Myb can bind to wild-type but not point mutated Myb binding sequences of the PMCA1 promoter. Transient transfection assays using cell cycle-synchronized vascular smooth muscle cells (VSMC) and PMCA1 promoter-luciferase constructs showed a 2-fold decrease in reporter activity at G1/S as compared with G0. Overexpression of wild-type c-Myb severely repressed PMCA1 promoter activity at both G0 and G1/S while co-transfection of a dominant negative c-Myb, or a construct encoding an anti-c-Myb neutralizing antibody, completely abolished the repression seen at G1/S. Single nucleotide substitutions in the first, second, or both Myb-binding sites alleviated the G1/S-associated repression of PMCA1 promoter activity in transformed rat VSMC and primary mouse VSMC cultures. We conclude that c-Myb mediates G1/S-associated transcriptional repression of the PMCA1 Ca2+ pump in rodent VSMC by direct binding to the PMCA1 promoter.

* This work was supported in part by Medical Research Council of Canada Grants CL42617 and MT14648, Heart & Stroke Foundation of Ontario Grant NA3636, and the Allan E. Tiffin Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF162783.

Dagger Recipient of a Clinician Scientist Award from the Medical Research Council of Canada. To whom correspondence should be addressed: EN 12-221, 200 Elizabeth St., Toronto General Hospital, Toronto, ON M5G 2C4, Canada. Tel.: 416-340-3188; Fax: 416-340-4021; E-mail: mansoor.husain@utoronto.ca.

Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Circ. Res.Home page
K. M. Kolodziejska, M.H. Noyan-Ashraf, A. Nagy, A. Bacon, J. Frampton, H.-B. Xin, M. I. Kotlikoff, and M. Husain
c-Myb-Dependent Smooth Muscle Cell Differentiation
Circ. Res., March 14, 2008; 102(5): 554 - 561.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Afroze, A. M. Sadi, M. A. Momen, S. Gu, S. Heximer, and M. Husain
c-Myb-Dependent Inositol 1,4,5-Trisphosphate Receptor Type-1 Expression in Vascular Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1305 - 1311.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Fan, M. Zhan, J. Shen, J. L. Martindale, X. Yang, T. Kawai, and M. Gorospe
En masse nascent transcription analysis to elucidate regulatory transcription factors
Nucleic Acids Res., March 15, 2006; 34(5): 1492 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Afroze, L. L. Yang, C. Wang, R. Gros, W. Kalair, A. N. Hoque, I. N. Mungrue, Z. Zhu, and M. Husain
Calcineurin-independent regulation of plasma membrane Ca2+ ATPase-4 in the vascular smooth muscle cell cycle
Am J Physiol Cell Physiol, July 1, 2003; 285(1): C88 - C95.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-E. Chaboute, B. Clement, and G. Philipps
S Phase and Meristem-specific Expression of the Tobacco RNR1b Gene Is Mediated by an E2F Element Located in the 5' Leader Sequence
J. Biol. Chem., May 10, 2002; 277(20): 17845 - 17851.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. E. Strehler and D. A. Zacharias
Role of Alternative Splicing in Generating Isoform Diversity Among Plasma Membrane Calcium Pumps
Physiol Rev, January 1, 2001; 81(1): 21 - 50.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2000 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement