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J Biol Chem, Vol. 275, Issue 13, 9157-9162, March 31, 2000
From the Department of Anatomy, Mie University School of Medicine,
Tsu, Mie 514-8507, Japan
Proline- and alanine-rich Ste20-related kinase
(PASK) is a Ste20-related protein kinase isolated from rat brain. Cell
fractionation studies showed that PASK was present both in the cytosol
and in Triton X-100-insoluble cytoskeletal fraction in rat tissues. In brain, PASK associated with protein complexes that contained actin and
tubulin, confirming the association of PASK with the cytoskeleton in vivo. Glutathione S-transferase-PASK fusion
protein cosedimented with F-actin, indicating that PASK binds to
F-actin. In contrast to rat tissues, PASK was detected only in the
Triton X-100-soluble cytosolic fraction in cultured PC12 and NIH 3T3
cells. Cytosolic PASK translocated to the cytoskeleton when these cells
were stimulated with severe cellular stresses such as hypertonic sodium
chloride, hydrogen peroxide, and heat shock at 45 °C. Our results
suggest that PASK may be involved in the regulation of the cytoskeleton in response to cellular stresses such as hyperosmotic shock.
Proline- and Alanine-rich Ste20-related Kinase Associates with
F-actin and Translocates from the Cytosol to Cytoskeleton upon Cellular
Stresses*
,
*
This work was supported in part by grants-in-aid from the
Ministry of Education, Science, and Culture of Japan and the Mie Medical Research Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Anatomy, Mie
University School of Medicine, Edobashi 2-174, Tsu, Mie 514-8507, Japan. Tel.: 81-59-232-1111 (ext. 6322); Fax: 81-59-231-5219; E-mail:
ushiro@doc.medic.mie-u.ac.jp.
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