HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schmitt, E. K. Articles by Kück, U. Search for Related Content PubMed PubMed Citation Articles by Schmitt, E. K. Articles by Kück, U. Social Bookmarking                      What's this?

J Biol Chem, Vol. 275, Issue 13, 9348-9357, March 31, 2000

The Fungal CPCR1 Protein, Which Binds Specifically to -Lactam Biosynthesis Genes, Is Related to Human Regulatory Factor X Transcription Factors^*

Esther K. Schmitt and Ulrich Kück^§

From the Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, D-44780 Bochum, Germany

Here we report the isolation and characterization of a novel transcription factor from the cephalosporin C-producing fungus Acremonium chrysogenum. We have identified a protein binding site in the promoter of the -lactam biosynthesis gene pcbC, located 418 nucleotides upstream of the translational start. Using the yeast one-hybrid system, we succeeded in isolating a cDNA clone encoding a polypeptide, which binds specifically to the pcbC promoter. The polypeptid shows significant sequence homology to human transcription factors of the regulatory factor X (RFX) family and was designated CPCR1. A high degree of CPCR1 binding specificity was observed in in vivo and in vitro experiments using mutated versions of the DNA binding site. The A. chrysogenum RFX protein CPCR1 recognizes an imperfect palindrome, which resembles binding sites of human RFX transcription factors. One- and two-hybrid experiments with truncated versions of CPCR1 showed that the protein forms a DNA binding homodimer. Nondenaturing electrophoresis revealed that the CPCR1 protein exists in vitro solely in a multimeric, probably dimeric, state. Finally, we isolated a homologue of the cpcR1 gene from the penicillin-producing fungus Penicillium chrysogenum and determined about 60% identical amino acid residues in the DNA binding domain of both fungal RFX proteins, which show an overall amino acid sequence identity of 29%.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBank^TM/EMBL Data Bank with accession number(s) AJ132014 (AcCPCR1) and AJ243296 (PcRFX1).

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				Y. Lubelsky, N. Reuven, and Y. Shaul Autorepression of Rfx1 Gene Expression: Functional Conservation from Yeast to Humans in Response to DNA Replication Arrest Mol. Cell. Biol., December 1, 2005; 25(23): 10665 - 10673. [Abstract] [Full Text] [PDF]

				R. Araki, H. Takahashi, R. Fukumura, F. Sun, N. Umeda, M. Sujino, S.-I. T. Inouye, T. Saito, and M. Abe Restricted Expression and Photic Induction of a Novel Mouse Regulatory Factor X4 Transcript in the Suprachiasmatic Nucleus J. Biol. Chem., March 12, 2004; 279(11): 10237 - 10242. [Abstract] [Full Text] [PDF]

				E. K. Schmitt, A. Bunse, D. Janus, B. Hoff, E. Friedlin, H. Kurnsteiner, and U. Kuck Winged Helix Transcription Factor CPCR1 Is Involved in Regulation of {beta}-Lactam Biosynthesis in the Fungus Acremonium chrysogenum Eukaryot. Cell, February 1, 2004; 3(1): 121 - 134. [Abstract] [Full Text] [PDF]

				K. Morotomi-Yano, K.-i. Yano, H. Saito, Z. Sun, A. Iwama, and Y. Miki Human Regulatory Factor X 4 (RFX4) Is a Testis-specific Dimeric DNA-binding Protein That Cooperates with Other Human RFX Members J. Biol. Chem., January 4, 2002; 277(1): 836 - 842. [Abstract] [Full Text]