|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 14, 10218-10227, April 7, 2000
From the Instituto de Investigaciones Biotecnológicas,
Universidad Nacional de General San Martín, C.C. 30, 1650 San Martín, Pcia. de Buenos Aires, Argentina
Trypanosoma cruzi has a
complex mucin gene family of 500 members with hypervariable regions
expressed preferentially in vertebrate associated stages of the
parasite. In this work, a novel mucin-type gene family is reported,
composed of two groups of genes organized in independent tandems and
having very short open reading frames. The structures of deduced
proteins share the N and C termini but differ in central regions. One
group has repeats with the consensus Lys-Asn-Thr7-Ser-Thr3-Ser(Ser/Lys)-Ala-Pro and
the other a Thr-rich sequence of the type
Asp-Gln-Thr17-20-Asn-Ala-Pro-Ala-Lys-Asp-Thr5-7-Asn-Ala-Pro-Ala-Lys.
In both cases, expected mature core proteins are around 7 kDa. Both
groups, named L and S, respectively, differ in the structure of genomic
loci and mRNA, with differential blocks in the 3'-untranslated
region. The highest mRNA level for S and L groups are in the
epimastigote stage but they show distinct developmentally regulated
patterns. Transcripts are short lived and their steady-state abundance
is regulated post-transcriptionally with increased mRNA stability
in insect stage epimastigote. AU-rich sequences, similar to ARE motives
known to cause mRNA instability in higher eukaryotes, are present
in the 3'-untranslated region of the transcripts. In transfection
experiments this sequence is shown to be functional for the L group
destabilizing its mRNA in a stage-specific manner. Furthermore, an
effect of this AU-rich region on translation efficiency is shown. To
our knowledge, this is the first time that a functional ARE
sequence-dependent post-transcriptional regulation
mechanism is reported in a lower eukaryote.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF203085 to AF203105.
AU-rich Elements in the 3'-Untranslated Region of a New
Mucin-type Gene Family of Trypanosoma cruzi Confers
mRNA Instability and Modulates Translation Efficiency*
§,§,
*
This work was supported by grants from the UNDP/World
Bank/WHO Special Program for Research and Training in Tropical
Diseases, the Swedish Agency for Research Cooperation with Developing
Countries, the Consejo Nacional de Investigaciones Cientificas y
Técnicas (CONICET), Argentina, the Agencia Nacional de
Promoción Científica y Tecnología, Argentina, and
Fundación Antorchas, Argentina.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Contributed equally to the results of this work.
§
Fellow of the Consejo Nacional de Investigaciones Cientificas y
Técnicas, Argentina.
¶
Researcher for the Consejo Nacional de Investigaciones
Cientificas y Técnicas, Argentina.
Supported in part by an International Research Scholar Grant
from the Howard Hughes Medical Institute. To whom correspondence should
be addressed: Instituto de Investigaciones
Biotecnológicas-Universidad Nacional de General San
Martín, Av. Gral. Paz s/n, INTI, Edificio 24, 1650-San
Martín, Pcia. de Buenos Aires, Argentina. Tel.: 54-11-4752-9639; Fax: 54-11-4752-0021; E-mail:
cfrasch@iib.unsam.edu.ar.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. C. Bartholomeu, G. C. Cerqueira, A. C. A. Leao, W. D. daRocha, F. S. Pais, C. Macedo, A. Djikeng, S. M. R. Teixeira, and N. M. El-Sayed Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi Nucleic Acids Res., June 1, 2009; 37(10): 3407 - 3417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Haile, A. Dupe, and B. Papadopoulou Deadenylation-independent stage-specific mRNA degradation in Leishmania Nucleic Acids Res., March 1, 2008; 36(5): 1634 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hartmann, C. Benz, S. Brems, L. Ellis, V.-D. Luu, M. Stewart, I. D'Orso, C. Busold, K. Fellenberg, A. C. C. Frasch, et al. Small Trypanosome RNA-Binding Proteins TbUBP1 and TbUBP2 Influence Expression of F-Box Protein mRNAs in Bloodstream Trypanosomes Eukaryot. Cell, November 1, 2007; 6(11): 1964 - 1978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Jager, J. G. De Gaudenzi, A. Cassola, I. D'Orso, and A. C. Frasch Inaugural Article: mRNA maturation by two-step trans-splicing/polyadenylation processing in trypanosomes PNAS, February 13, 2007; 104(7): 2035 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mayho, K. Fenn, P. Craddy, S. Crosthwaite, and K. Matthews Post-transcriptional control of nuclear-encoded cytochrome oxidase subunits in Trypanosoma brucei: evidence for genome-wide conservation of life-cycle stage-specific regulatory elements Nucleic Acids Res., October 6, 2006; 34(18): 5312 - 5324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Subramaniam, K. Chen, K. Joseph, J. R. Raymond, and B. G. Tholanikunnel The 3'-Untranslated Region of the {beta}2-Adrenergic Receptor mRNA Regulates Receptor Synthesis J. Biol. Chem., June 25, 2004; 279(26): 27108 - 27115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Buscaglia, V. A. Campo, J. M. Di Noia, A. C. T. Torrecilhas, C. R. De Marchi, M. A. J. Ferguson, A. C. C. Frasch, and I. C. Almeida The Surface Coat of the Mammal-dwelling Infective Trypomastigote Stage of Trypanosoma cruzi Is Formed by Highly Diverse Immunogenic Mucins J. Biol. Chem., April 16, 2004; 279(16): 15860 - 15869. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. MILONE, J. WILUSZ, and V. BELLOFATTO Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p RNA, March 1, 2004; 10(3): 448 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. K. Avliyakulov, J. C. Hines, and D. S. Ray Sequence Elements in both the Intergenic Space and the 3' Untranslated Region of the Crithidia fasciculata KAP3 Gene Are Required for Cell Cycle Regulation of KAP3 mRNA Eukaryot. Cell, August 1, 2003; 2(4): 671 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mittra, K. M. Sinha, J. C. Hines, and D. S. Ray Presence of Multiple mRNA Cycling Sequence Element-binding Proteins in Crithidia fasciculata J. Biol. Chem., July 11, 2003; 278(29): 26564 - 26571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. De Gaudenzi, I. D'Orso, and A. C. C. Frasch RNA Recognition Motif-type RNA-binding Proteins in Trypanosoma cruzi Form a Family Involved in the Interaction with Specific Transcripts in Vivo J. Biol. Chem., May 23, 2003; 278(21): 18884 - 18894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Manque, I. Neira, V. D. Atayde, E. Cordero, A. T. Ferreira, J. F. da Silveira, M. Ramirez, and N. Yoshida Cell Adhesion and Ca2+ Signaling Activity in Stably Transfected Trypanosoma cruzi Epimastigotes Expressing the Metacyclic Stage-Specific Surface Molecule gp82 Infect. Immun., March 1, 2003; 71(3): 1561 - 1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. D'Orso and A. C. C. Frasch TcUBP-1, an mRNA Destabilizing Factor from Trypanosomes, Homodimerizes and Interacts with Novel AU-rich Element- and Poly(A)-binding Proteins Forming a Ribonucleoprotein Complex J. Biol. Chem., December 20, 2002; 277(52): 50520 - 50528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Quijada, C. Guerra-Giraldez, M. Drozdz, C. Hartmann, H. Irmer, C. Ben-Dov, M. Cristodero, M. Ding, and C. Clayton Expression of the human RNA-binding protein HuR in Trypanosoma brucei increases the abundance of mRNAs containing AU-rich regulatory elements Nucleic Acids Res., October 15, 2002; 30(20): 4414 - 4424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Milone, J. Wilusz, and V. Bellofatto Identification of mRNA decapping activities and an ARE-regulated 3' to 5' exonuclease activity in trypanosome extracts Nucleic Acids Res., September 15, 2002; 30(18): 4040 - 4050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Boucher, Y. Wu, C. Dumas, M. Dube, D. Sereno, M. Breton, and B. Papadopoulou A Common Mechanism of Stage-regulated Gene Expression in Leishmania Mediated by a Conserved 3'-Untranslated Region Element J. Biol. Chem., May 24, 2002; 277(22): 19511 - 19520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Di Noia, C. A. Buscaglia, C. R. De Marchi, I. C. Almeida, and A. C.C. Frasch A Trypanosoma cruzi Small Surface Molecule Provides the First Immunological Evidence that Chagas' Disease Is Due to a Single Parasite Lineage J. Exp. Med., February 11, 2002; 195(4): 401 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Argibay, J. M. Di Noia, A. Hidalgo, E. Mocetti, M. Barbich, A. S. Lorenti, D. Bustos, M. Tambutti, S. H. Hyon, A. C.C. Frasch, et al. Trypanosoma cruzi surface mucin TcMuc-e2 expressed on higher eukaryotic cells induces human T cell anergy, which is reversible Glycobiology, January 1, 2002; 12(1): 25 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Brooks, H. Denise, G. D. Westrop, G. H. Coombs, and J. C. Mottram The Stage-regulated Expression of Leishmania mexicana CPB Cysteine Proteases Is Mediated by an Intercistronic Sequence Element J. Biol. Chem., December 7, 2001; 276(50): 47061 - 47069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Irmer and C. Clayton Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region Nucleic Acids Res., November 15, 2001; 29(22): 4707 - 4715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. D. Pollevick, J. M. Di Noia, M. L. Salto, C. Lima, M. S. Leguizamon, R. M. de Lederkremer, and A. C. C. Frasch Trypanosoma cruzi Surface Mucins with Exposed Variant Epitopes J. Biol. Chem., September 1, 2000; 275(36): 27671 - 27680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. D'Orso and A. C. C. Frasch Functionally Different AU- and G-rich cis-Elements Confer Developmentally Regulated mRNA Stability in Trypanosoma cruzi by Interaction with Specific RNA-binding Proteins J. Biol. Chem., May 4, 2001; 276(19): 15783 - 15793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. D'Orso and A. C. C. Frasch TcUBP-1, a Developmentally Regulated U-rich RNA-binding Protein Involved in Selective mRNA Destabilization in Trypanosomes J. Biol. Chem., September 7, 2001; 276(37): 34801 - 34809. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |