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J Biol Chem, Vol. 275, Issue 14, 10256-10264, April 7, 2000
From the Institute of Cell Biology, ETH-Zürich
Hönggerberg, CH-8093 Zürich, Switzerland
Myomesin is a structural component of the M-band
that is expressed in all types of striated muscle. Its primary function
may be the maintenance of the thick filament lattice and its anchoring to the elastic filament system composed of titin. Different myomesin isoforms have been described in chicken and mice, but no particular function has been assigned to them. Here we investigate the
spatio-temporal expression pattern of myomesin isoforms by means of
reverse transcriptase-polymerase chain reaction and isoform-specific
antibodies. We find that two alternative splicing events give rise to
four myomesin isoforms in chicken contrary to only one splicing event
with two possible isoforms in mice. A splicing event at the C terminus
results in two splice variants termed H-myomesin and S-myomesin, which
represent the major myomesin species in heart and skeletal muscle of
avian species, respectively. In contrast, in mammalian heart and
skeletal muscle only S-myomesin is expressed. In embryonic heart of
birds and mammals, alternative splicing in the central part of the
molecule gives rise to the isoform that we termed EH-myomesin. It
represents the major myomesin isoform at early embryonic stages of
heart but is rapidly down-regulated around birth. Thus, the strict
developmental regulation of the EH-myomesin makes it an ideally suited
marker for embryonic heart.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF185573 and AF185572.
A Novel Marker for Vertebrate Embryonic Heart, the
EH-myomesin Isoform*
,
*
This work was supported in part by Swiss National Science
Foundation Grant 31.52417/97.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Supported by the Stipendienkommission of the ETH.
§
To whom correspondence should be addressed. Tel.: 0041-1-6333359;
Fax 0041-1-6331069; E-mail jcp@cell.biol.ethz.ch.
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