HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Boneca, I. G. Articles by Tomasz, A. Search for Related Content PubMed PubMed Citation Articles by Boneca, I. G. Articles by Tomasz, A. Social Bookmarking                      What's this?

J Biol Chem, Vol. 275, Issue 14, 9910-9918, April 7, 2000

Characterization of Staphylococcus aureus Cell Wall Glycan Strands, Evidence for a New -N-Acetylglucosaminidase Activity^*

Ivo G. Boneca^§, Zhi-Heng Huang^¶, Douglas A. Gage^¶, and Alexander Tomasz

From the  Laboratory of Microbiology, The Rockefeller University, New York, New York 10021 and the ^¶ Department of Biochemistry, Michigan State University, National Institutes of Health Mass Spectrometry Facility, East Lansing, Michigan 48824-1319

Using sequential digestion with the glycyl-glycine endopeptidase lysostaphin followed by the pneumococcal N-acetylmuramyl-L-alanine amidase (amidase), the glycan strands of the peptidoglycan of Staphylococcus aureus were purified and analyzed by a combination of reverse-phase-high pressure liquid chromatography (HPLC) and mass spectrometry. Reverse-phase-HPLC resolved the glycan strands to a family of major peaks, which represented oligosaccharides composed of repeating disaccharide units (N-acetylglucosamine-[-1,4]-N-acetylmuramic acid) with different degrees of polymerization and terminating with N-acetylmuramic acid residues at the reducing ends. The method allowed separation of strands up to 23-26 disaccharide units with a predominant length between 3 and 10 and an average degree of polymerization of ~6. Glycan strands with a higher degree of polymerization (>26 disaccharide units) represented 10-15% of the total UV absorbing glycan material. A unique feature of the staphylococcal glycan strands was the presence of minor satellite peaks that were present throughout the HPLC elution profile eluting either just prior or shortly after the major oligosaccharide peaks. A number of observations including mass spectrometric analysis suggest that the satellites are the products of an N-acetylglucosaminidase activity that differs from the atl gene product and that appears to be involved with modification of the glycan strand structure.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				N. K. Bui, J. Gray, H. Schwarz, P. Schumann, D. Blanot, and W. Vollmer The Peptidoglycan Sacculus of Myxococcus xanthus Has Unusual Structural Features and Is Degraded during Glycerol-Induced Myxospore Development J. Bacteriol., January 15, 2009; 191(2): 494 - 505. [Abstract] [Full Text] [PDF]

				E. J. Hayhurst, L. Kailas, J. K. Hobbs, and S. J. Foster Cell wall peptidoglycan architecture in Bacillus subtilis PNAS, September 23, 2008; 105(38): 14603 - 14608. [Abstract] [Full Text] [PDF]

				S. Dubrac, I. G. Boneca, O. Poupel, and T. Msadek New Insights into the WalK/WalR (YycG/YycF) Essential Signal Transduction Pathway Reveal a Major Role in Controlling Cell Wall Metabolism and Biofilm Formation in Staphylococcus aureus J. Bacteriol., November 15, 2007; 189(22): 8257 - 8269. [Abstract] [Full Text] [PDF]

				M. R. Stapleton, M. J. Horsburgh, E. J. Hayhurst, L. Wright, I.-M. Jonsson, A. Tarkowski, J. F. Kokai-Kun, J. J. Mond, and S. J. Foster Characterization of IsaA and SceD, Two Putative Lytic Transglycosylases of Staphylococcus aureus J. Bacteriol., October 15, 2007; 189(20): 7316 - 7325. [Abstract] [Full Text] [PDF]

				A. C. DeDent, M. McAdow, and O. Schneewind Distribution of Protein A on the Surface of Staphylococcus aureus J. Bacteriol., June 15, 2007; 189(12): 4473 - 4484. [Abstract] [Full Text] [PDF]

				I. Valera, A. G. Vigo, S. Alonso, L. Barbolla, M. S. Crespo, and N. Fernandez Peptidoglycan and mannose-based molecular patterns trigger the arachidonic acid cascade in human polymorphonuclear leukocytes J. Leukoc. Biol., April 1, 2007; 81(4): 925 - 933. [Abstract] [Full Text] [PDF]

				C. Chaput, A. Labigne, and I. G. Boneca Characterization of Helicobacter pylori Lytic Transglycosylases Slt and MltD J. Bacteriol., January 15, 2007; 189(2): 422 - 429. [Abstract] [Full Text] [PDF]

				V. R. F. Matias and T. J. Beveridge Native Cell Wall Organization Shown by Cryo-Electron Microscopy Confirms the Existence of a Periplasmic Space in Staphylococcus aureus J. Bacteriol., February 1, 2006; 188(3): 1011 - 1021. [Abstract] [Full Text] [PDF]

				D.-J. Scheffers and M. G. Pinho Bacterial Cell Wall Synthesis: New Insights from Localization Studies Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 585 - 607. [Abstract] [Full Text] [PDF]

				A. Severin, S. W. Wu, K. Tabei, and A. Tomasz High-Level {beta}-Lactam Resistance and Cell Wall Synthesis Catalyzed by the mecA Homologue of Staphylococcus sciuri Introduced into Staphylococcus aureus J. Bacteriol., October 1, 2005; 187(19): 6651 - 6658. [Abstract] [Full Text] [PDF]

				L. A. Marraffini and O. Schneewind Anchor Structure of Staphylococcal Surface Proteins: V. ANCHOR STRUCTURE OF THE SORTASE B SUBSTRATE IsdC J. Biol. Chem., April 22, 2005; 280(16): 16263 - 16271. [Abstract] [Full Text] [PDF]

				A. Severin, S. W. Wu, K. Tabei, and A. Tomasz Penicillin-Binding Protein 2 Is Essential for Expression of High-Level Vancomycin Resistance and Cell Wall Synthesis in Vancomycin- Resistant Staphylococcus aureus Carrying the Enterococcal vanA Gene Complex Antimicrob. Agents Chemother., December 1, 2004; 48(12): 4566 - 4573. [Abstract] [Full Text] [PDF]

				B. A. Dmitriev, F. V. Toukach, O. Holst, E. T. Rietschel, and S. Ehlers Tertiary Structure of Staphylococcus aureus Cell Wall Murein J. Bacteriol., November 1, 2004; 186(21): 7141 - 7148. [Abstract] [Full Text] [PDF]

				A. Touhami, M. H. Jericho, and T. J. Beveridge Atomic Force Microscopy of Cell Growth and Division in Staphylococcus aureus J. Bacteriol., June 1, 2004; 186(11): 3286 - 3295. [Abstract] [Full Text] [PDF]

				A. Severin, K. Tabei, F. Tenover, M. Chung, N. Clarke, and A. Tomasz High Level Oxacillin and Vancomycin Resistance and Altered Cell Wall Composition in Staphylococcus aureus Carrying the Staphylococcal mecA and the Enterococcal vanA Gene Complex J. Biol. Chem., January 30, 2004; 279(5): 3398 - 3407. [Abstract] [Full Text] [PDF]

				B. A. Dmitriev, F. V. Toukach, K.-J. Schaper, O. Holst, E. T. Rietschel, and S. Ehlers Tertiary Structure of Bacterial Murein: the Scaffold Model J. Bacteriol., June 1, 2003; 185(11): 3458 - 3468. [Abstract] [Full Text] [PDF]

				H. Komatsuzawa, K. Ohta, S. Yamada, K. Ehlert, H. Labischinski, J. Kajimura, T. Fujiwara, and M. Sugai Increased Glycan Chain Length Distribution and Decreased Susceptibility to Moenomycin in a Vancomycin-Resistant Staphylococcus aureus Mutant Antimicrob. Agents Chemother., January 1, 2002; 46(1): 75 - 81. [Abstract] [Full Text] [PDF]

				M. G. Pinho, H. de Lencastre, and A. Tomasz An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci PNAS, August 17, 2001; (2001) 191260798. [Abstract] [Full Text] [PDF]

				M. G. Pinho, H. de Lencastre, and A. Tomasz An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci PNAS, September 11, 2001; 98(19): 10886 - 10891. [Abstract] [Full Text] [PDF]