|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 15, 11229-11234, April 14, 2000
From the Department of Bioscience and Biotechnology, Division of
Bioresource and Bioenvironmental Sciences, Graduate School Kyushu
University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan and
the We report here the molecular cloning, sequencing,
and expression of the gene encoding the mouse neutral ceramidase, which has been proposed to function in sphingolipid signaling. A full-length cDNA encoding the neutral ceramidase was cloned from a cDNA
library of mouse liver using the partial amino acid sequences of the
purified mouse liver ceramidase. The open reading frame of 2,268 nucleotides encoded a polypeptide of 756 amino acids having nine
putative N-glycosylation sites. Northern blot analysis
revealed that the mRNA of the ceramidase was expressed widely in
mouse tissues, with especially strong signals found in the liver and
kidney. The ceramidase activity of lysates of CHOP cells increased more than 900-fold when the cells were transformed with a plasmid containing the cDNA encoding ceramidase. We also cloned the ceramidase
homologue from the cDNA library of mouse brain and found that the
sequence of the open reading frame, but not the 5'-noncoding region,
was identical to that of the liver. Interestingly, phylogenetic
analysis of various ceramidases clearly indicated that neutral/alkaline ceramidases form a novel but highly conserved gene family that is
evolutionarily different from lysosomal acid ceramidases.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AB037181 (mouse brain) and AB037111 (mouse liver).
Molecular Cloning of the Full-length cDNA Encoding Mouse
Neutral Ceramidase
A NOVEL BUT HIGHLY CONSERVED GENE FAMILY OF NEUTRAL/ALKALINE
CERAMIDASES*
,, and Biotechnology Research Laboratories of Takara Shuzo
Co., Ltd., Seta 3-4-1, Otsu, Shiga 520-2134, Japan
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Inoue, N. Okino, Y. Kakuta, A. Hijikata, H. Okano, H. M. Goda, M. Tani, N. Sueyoshi, K. Kambayashi, H. Matsumura, et al. Mechanistic Insights into the Hydrolysis and Synthesis of Ceramide by Neutral Ceramidase J. Biol. Chem., April 3, 2009; 284(14): 9566 - 9577. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhang, R. A. Barthelson, G. M. Lambert, and D. W. Galbraith Global Characterization of Cell-Specific Gene Expression through Fluorescence-Activated Sorting of Nuclei Plant Physiology, May 1, 2008; 147(1): 30 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Okino and M. Ito Ceramidase Enhances Phospholipase C-induced Hemolysis by Pseudomonas aeruginosa J. Biol. Chem., March 2, 2007; 282(9): 6021 - 6030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. X. Wu, C. F. Snook, M. Tani, E. E. Bullesbach, and Y. A. Hannun Large-scale purification and characterization of recombinant Pseudomonas ceramidase: regulation by calcium J. Lipid Res., March 1, 2007; 48(3): 600 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Xu, J. Jin, W. Hu, W. Sun, J. Bielawski, Z. Szulc, T. Taha, L. M. Obeid, and C. Mao Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P FASEB J, September 1, 2006; 20(11): 1813 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kono, J. L. Dreier, J. M. Ellis, M. L. Allende, D. N. Kalkofen, K. M. Sanders, J. Bielawski, A. Bielawska, Y. A. Hannun, and R. L. Proia Neutral Ceramidase Encoded by the Asah2 Gene Is Essential for the Intestinal Degradation of Sphingolipids J. Biol. Chem., March 17, 2006; 281(11): 7324 - 7331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mitsutake and Y. Igarashi Calmodulin Is Involved in the Ca2+-dependent Activation of Ceramide Kinase as a Calcium Sensor J. Biol. Chem., December 9, 2005; 280(49): 40436 - 40441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tani, Y. Igarashi, and M. Ito Involvement of Neutral Ceramidase in Ceramide Metabolism at the Plasma Membrane and in Extracellular Milieu J. Biol. Chem., November 4, 2005; 280(44): 36592 - 36600. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Osawa, H. Uchinami, J. Bielawski, R. F. Schwabe, Y. A. Hannun, and D. A. Brenner Roles for C16-ceramide and Sphingosine 1-Phosphate in Regulating Hepatocyte Apoptosis in Response to Tumor Necrosis Factor-{alpha} J. Biol. Chem., July 29, 2005; 280(30): 27879 - 27887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yoshimura, M. Tani, N. Okino, H. Iida, and M. Ito Molecular Cloning and Functional Analysis of Zebrafish Neutral Ceramidase J. Biol. Chem., October 15, 2004; 279(42): 44012 - 44022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Horibata, K. Sakaguchi, N. Okino, H. Iida, M. Inagaki, T. Fujisawa, Y. Hama, and M. Ito Unique Catabolic Pathway of Glycosphingolipids in a Hydrozoan, Hydra magnipapillata, Involving Endoglycoceramidase J. Biol. Chem., August 6, 2004; 279(32): 33379 - 33389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tani, N. Okino, N. Sueyoshi, and M. Ito Conserved Amino Acid Residues in the COOH-terminal Tail Are Indispensable for the Correct Folding and Localization and Enzyme Activity of Neutral Ceramidase J. Biol. Chem., July 9, 2004; 279(28): 29351 - 29358. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Monick, R. K. Mallampalli, M. Bradford, D. McCoy, T. J. Gross, D. M. Flaherty, L. S. Powers, K. Cameron, S. Kelly, A. H. Merrill Jr., et al. Cooperative Prosurvival Activity by ERK and Akt in Human Alveolar Macrophages is Dependent on High Levels of Acid Ceramidase Activity J. Immunol., July 1, 2004; 173(1): 123 - 135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Monick, K. Cameron, L. S. Powers, N. S. Butler, D. McCoy, R. K. Mallampalli, and G. W. Hunninghake Sphingosine Kinase Mediates Activation of Extracellular Signal-Related Kinase and Akt by Respiratory Syncytial Virus Am. J. Respir. Cell Mol. Biol., June 1, 2004; 30(6): 844 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. He, N. Okino, R. Dhami, A. Dagan, S. Gatt, H. Schulze, K. Sandhoff, and E. H. Schuchman Purification and Characterization of Recombinant, Human Acid Ceramidase: CATALYTIC REACTIONS AND INTERACTIONS WITH ACID SPHINGOMYELINASE J. Biol. Chem., August 29, 2003; 278(35): 32978 - 32986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mao, R. Xu, Z. M. Szulc, J. Bielawski, K. P. Becker, A. Bielawska, S. H. Galadari, W. Hu, and L. M. Obeid Cloning and Characterization of a Mouse Endoplasmic Reticulum Alkaline Ceramidase: AN ENZYME THAT PREFERENTIALLY REGULATES METABOLISM OF VERY LONG CHAIN CERAMIDES J. Biol. Chem., August 15, 2003; 278(33): 31184 - 31191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Okino, X. He, S. Gatt, K. Sandhoff, M. Ito, and E. H. Schuchman The Reverse Activity of Human Acid Ceramidase J. Biol. Chem., August 8, 2003; 278(32): 29948 - 29953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tani, H. Iida, and M. Ito O-Glycosylation of Mucin-like Domain Retains the Neutral Ceramidase on the Plasma Membranes as a Type II Integral Membrane Protein J. Biol. Chem., March 14, 2003; 278(12): 10523 - 10530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Franzen, D. Fabbro, A. Aschrafi, J. Pfeilschifter, and A. Huwiler Nitric Oxide Induces Degradation of the Neutral Ceramidase in Rat Renal Mesangial Cells and Is Counterregulated by Protein Kinase C J. Biol. Chem., November 22, 2002; 277(48): 46184 - 46190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. El Bawab, J. Usta, P. Roddy, Z. M. Szulc, A. Bielawska, and Y. A. Hannun Substrate specificity of rat brain ceramidase J. Lipid Res., January 1, 2002; 43(1): 141 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Levade, N. Auge, R. J. Veldman, O. Cuvillier, A. Negre-Salvayre, and R. Salvayre Sphingolipid Mediators in Cardiovascular Cell Biology and Pathology Circ. Res., November 23, 2001; 89(11): 957 - 968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Kraj, R. Pacholczyk, H. Ignatowicz, P. Kisielow, P. Jensen, and L. Ignatowicz Positive Selection of CD4+ T Cells Is Induced In Vivo by Agonist and Inhibited by Antagonist Peptides J. Exp. Med., August 13, 2001; 194(4): 407 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mao, R. Xu, A. Bielawska, Z. M. Szulc, and L. M. Obeid Cloning and Characterization of a Saccharomyces cerevisiae Alkaline Ceramidase with Specificity for Dihydroceramide J. Biol. Chem., September 29, 2000; 275(40): 31369 - 31378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. El Bawab, H. Birbes, P. Roddy, Z. M. Szulc, A. Bielawska, and Y. A. Hannun Biochemical Characterization of the Reverse Activity of Rat Brain Ceramidase. A CoA-INDEPENDENT AND FUMONISIN B1-INSENSITIVE CERAMIDE SYNTHASE J. Biol. Chem., May 11, 2001; 276(20): 16758 - 16766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Franzen, A. Pautz, L. Brautigam, G. Geisslinger, J. Pfeilschifter, and A. Huwiler Interleukin-1beta Induces Chronic Activation and de Novo Synthesis of Neutral Ceramidase in Renal Mesangial Cells J. Biol. Chem., September 14, 2001; 276(38): 35382 - 35389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mitsutake, M. Tani, N. Okino, K. Mori, S. Ichinose, A. Omori, H. Iida, T. Nakamura, and M. Ito Purification, Characterization, Molecular Cloning, and Subcellular Distribution of Neutral Ceramidase of Rat Kidney J. Biol. Chem., July 6, 2001; 276(28): 26249 - 26259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mao, R. Xu, Z. M. Szulc, A. Bielawska, S. H. Galadari, and L. M. Obeid Cloning and Characterization of a Novel Human Alkaline Ceramidase. A MAMMALIAN ENZYME THAT HYDROLYZES PHYTOCERAMIDE J. Biol. Chem., July 6, 2001; 276(28): 26577 - 26588. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |