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J Biol Chem, Vol. 275, Issue 16, 11545-11552, April 21, 2000
From the SWI-SNF is an ATP-dependent chromatin
remodeling complex required for expression of a number of yeast genes.
Previous studies have suggested that SWI-SNF action may remove or
rearrange the histone H2A-H2B dimers or induce a novel alteration in
the histone octamer. Here, we have directly tested these and other
models by quantifying the remodeling activity of SWI-SNF on arrays of (H3-H4)2 tetramers, on nucleosomal arrays
reconstituted with disulfide-linked histone H3, and on arrays
reconstituted with histone H3 derivatives site-specifically modified at
residue 110 with the fluorescent probe
acetylethylenediamine-(1,5)-naphthol sulfonate. We find that SWI-SNF
can remodel (H3-H4)2 tetramers, although tetramers are poor
substrates for SWI-SNF remodeling compared with nucleosomal arrays.
SWI-SNF can also remodel nucleosomal arrays that harbor disulfide-linked (H3-H4)2 tetramers, indicating that
SWI-SNF action does not involve an obligatory disruption of the
tetramer. Finally, we find that although the fluorescence emission
intensity of acetylethylenediamine-(1,5)-naphthol sulfonate-modified
histone H3 is sensitive to octamer structure, SWI-SNF action does not
alter fluorescence emission intensity. These data suggest that
perturbation of the histone octamer is not a requirement or a
consequence of ATP-dependent nucleosome remodeling by
SWI-SNF.
Roles of the Histone H2A-H2B Dimers and the (H3-H4)2
Tetramer in Nucleosome Remodeling by the SWI-SNF Complex*
,¶
Program in Molecular Medicine and Department
of Biochemistry and Molecular Biology, University of Massachusetts
Medical School, Worcester, Massachusetts 01605 and the
§ Howard Hughes Medical Institute, Waksman Institute, and
Department of Chemistry, Rutgers University,
Piscataway, New Jersey 08854
*
This work was supported by National Institutes of Health
Grant GM49056-07 (to C. L. P.) and by a Howard Hughes Medical
Institute investigatorship (to R. H. E.).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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