|
|
|
|||||||
|
|||||||||
J Biol Chem, Vol. 275, Issue 16, 11880-11890, April 21, 2000
From the Department of Physiology and Pharmacology, the University
of Queensland, Brisbane, Queensland 4072, Australia
NaSi-1 is a Na+-sulfate
cotransporter expressed on the apical membrane of the renal proximal
tubule and plays an important role in sulfate reabsorption. To
understand the molecular mechanisms that mediate the regulation of
NaSi-1, we have isolated and characterized the mouse NaSi-1 cDNA
(mNaSi-1), gene (Nas1), and promoter region and determined
Nas1 chromosomal localization. The mNaSi-1 cDNA encodes
a protein of 594 amino acids with 13 putative transmembrane segments,
inducing high affinity Na+-dependent transport
of sulfate in Xenopus oocytes. Three different mNaSi-1
transcripts derived from alternative polyadenylation and splicing were
identified in kidney and intestine. The Nas1 gene is a
single copy gene comprising 15 exons spread over 75 kilobase pairs that
maps to mouse chromosome 6. Transcription initiation occurs from a
single site, 29 base pairs downstream to a TATA box-like sequence. The
promoter is AT-rich (61%), contains a number of well characterized
cis-acting elements, and can drive basal transcriptional
activity in opossum kidney cells but not in COS-1 or NIH3T3 cells. We
demonstrated that 1,25-dihydroxyvitamin D3 stimulated the
transcriptional activity of the Nas1 promoter in transiently transfected opossum kidney cells. This study represents the
first characterization of the genomic organization of a
Na+-sulfate cotransporter gene. It also provides the basis
for a detailed analysis of Nas1 gene regulation and the
tools required for assessing Nas1 role in sulfate
homeostasis using targeted gene manipulation in mice.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF199365, AF199366, AF199380, and AF200305-AF200319.
The Mouse Na+-Sulfate Cotransporter Gene
Nas1
CLONING, TISSUE DISTRIBUTION, GENE STRUCTURE, CHROMOSOMAL
ASSIGNMENT, AND TRANSCRIPTIONAL REGULATION BY VITAMIN D*
and
*
This work was supported in part by the National Health and
Medical Research Council of Australia (to D. M.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a University of Queensland postdoctoral research fellowship.
§
To whom correspondence should be addressed: Dept. of Physiology and
Pharmacology, the University of Queensland, Brisbane, Queensland 4072, Australia. Tel.: 61 7 3365 1400; Fax: 61 7 3365 1766; E-mail:
danielm@plpk.uq.edu.au.
Copyright © 2000 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P A Dawson, S Huxley, B Gardiner, T Tran, J L McAuley, S Grimmond, M A McGuckin, and D Markovich Reduced mucin sulfonation and impaired intestinal barrier function in the hyposulfataemic NaS1 null mouse Gut, July 1, 2009; 58(7): 910 - 919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Castrop and J. Schnermann Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and functional significance Am J Physiol Renal Physiol, October 1, 2008; 295(4): F859 - F866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Markovich, A. Romano, C. Storelli, and T. Verri Functional and structural characterization of the zebrafish Na+-sulfate cotransporter 1 (NaS1) cDNA and gene (slc13a1) Physiol Genomics, August 1, 2008; 34(3): 256 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Gisler, S. Kittanakom, D. Fuster, V. Wong, M. Bertic, T. Radanovic, R. A. Hall, H. Murer, J. Biber, D. Markovich, et al. Monitoring Protein-Protein Interactions between the Mammalian Integral Membrane Transporters and PDZ-interacting Partners Using a Modified Split-ubiquitin Membrane Yeast Two-hybrid System Mol. Cell. Proteomics, July 1, 2008; 7(7): 1362 - 1377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Dawson, B. Gardiner, S. Grimmond, and D. Markovich Transcriptional profile reveals altered hepatic lipid and cholesterol metabolism in hyposulfatemic NaS1 null mice Physiol Genomics, September 14, 2006; 26(2): 116 - 124. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakada, K. Zandi-Nejad, Y. Kurita, H. Kudo, V. Broumand, C. Y. Kwon, A. Mercado, D. B. Mount, and S. Hirose Roles of Slc13a1 and Slc26a1 sulfate transporters of eel kidney in sulfate homeostasis and osmoregulation in freshwater Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R575 - R585. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. G. Bolt, W. Liu, G. Qiao, J. Kong, W. Zheng, T. Krausz, G. Cs-Szabo, M. D. Sitrin, and Y. C. Li Critical role of vitamin D in sulfate homeostasis: regulation of the sodium-sulfate cotransporter by 1,25-dihydroxyvitamin D3 Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E744 - E749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Dawson, L. Beck, and D. Markovich Hyposulfatemia, growth retardation, reduced fertility, and seizures in mice lacking a functional NaSi-1 gene PNAS, November 11, 2003; 100(23): 13704 - 13709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li and A. M. Pajor Mutagenesis of the N-glycosylation site of hNaSi-1 reduces transport activity Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1188 - C1196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Dudas and J. L. Renfro Transepithelial sulfate transport by avian renal proximal tubule epithelium in primary culture Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2002; 283(6): R1354 - R1361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gamba Alternative splicing and diversity of renal transporters Am J Physiol Renal Physiol, November 1, 2001; 281(5): F781 - F794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Markovich Physiological Roles and Regulation of Mammalian Sulfate Transporters Physiol Rev, October 1, 2001; 81(4): 1499 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Cauvi, M.-C. Nlend, N. Venot, and O. Chabaud Sulfate transport in porcine thyroid cells. Effects of thyrotropin and iodide Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E440 - E448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Pajor, R. Gangula, and X. Yao Cloning and functional characterization of a high-affinity Na+/dicarboxylate cotransporter from mouse brain Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1215 - C1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Sagawa, I. M. Darling, H. Murer, and M. E. Morris Glucocorticoid-Induced Alterations of Renal Sulfate Transport J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 658 - 663. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |